BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,751	T→G	F144V (TTC→GTC)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,751	0	T	G	99.2%	859.1 / ‑4.9	235	F144V (TTC→GTC)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (100/133); total (101/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840

 gCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCa                                                                                        <  2:4549408/93‑1 (MQ=255)

 gCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCa                                                                                        >  1:2122740/1‑93 (MQ=255)

  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       >  1:1539361/1‑93 (MQ=255)

  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       <  1:5398959/93‑1 (MQ=255)

  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       <  1:5164504/93‑1 (MQ=255)

   cTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAc                                                                                      >  2:2288823/1‑93 (MQ=255)

    tGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACg                                                                                     <  2:3075226/93‑1 (MQ=255)

    tGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGCGAAATGGTCCGTGTTAATGATGGTCCGTTAGCTGACGTCAACg                                                                                     >  2:1969613/1‑93 (MQ=255)

     gcagcaGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACgg                                                                                    <  1:4255749/93‑1 (MQ=255)

     gcagcaGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACgg                                                                                    <  2:4617838/93‑1 (MQ=255)

      cagcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgt                                                                                   <  2:1824746/93‑1 (MQ=255)

        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 <  1:4399119/93‑1 (MQ=255)

        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  1:4788359/1‑93 (MQ=255)

        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 <  1:4695024/93‑1 (MQ=255)

        gcagGTTGGTGATAAGCCGCGCCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  1:5878603/1‑93 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                >  1:5024592/1‑93 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                <  1:5603734/93‑1 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                >  2:2638838/1‑93 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                <  1:800688/93‑1 (MQ=255)

          agGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttg                                                                               <  1:3586700/93‑1 (MQ=255)

           ggTTGGTGATAATCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  1:1011113/93‑1 (MQ=255)

           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              >  2:168435/1‑93 (MQ=255)

           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  1:2939800/93‑1 (MQ=255)

            gTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTgttgtt                                                                             >  1:1136397/1‑93 (MQ=255)

              tGGTGATAAGCCTCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           <  1:2426816/93‑1 (MQ=255)

               ggTGATAAGCCGCGTCCGAAAGCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaa                                                                          <  1:3257607/93‑1 (MQ=255)

               ggTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaa                                                                          >  2:3826943/1‑93 (MQ=255)

               ggTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGATGTTGATgaa                                                                          >  1:1355549/1‑93 (MQ=255)

                   aTAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaag                                                                      >  2:1043107/1‑93 (MQ=255)

                   aTAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaag                                                                      >  2:5195264/1‑93 (MQ=255)

                    tAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaagg                                                                     >  1:2266700/1‑92 (MQ=255)

                       gcagcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGa                                                                  <  1:6074302/90‑1 (MQ=255)

                       gCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGa                                                                  <  2:3637208/93‑1 (MQ=255)

                        ccGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAt                                                                 >  2:5792392/1‑93 (MQ=255)

                        ccGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAt                                                                 <  1:1662958/93‑1 (MQ=255)

                          gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTa                                                               <  1:780678/93‑1 (MQ=255)

                          gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTa                                                               >  2:533161/1‑93 (MQ=255)

                           cgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAc                                                              <  1:3018390/93‑1 (MQ=255)

                            gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACg                                                             <  1:1220700/93‑1 (MQ=255)

                            gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACg                                                             >  2:5213297/1‑93 (MQ=255)

                             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACga                                                            >  1:99431/1‑93 (MQ=255)

                                gAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaa                                                         >  2:4365185/1‑93 (MQ=255)

                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        >  2:2777463/1‑93 (MQ=255)

                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        <  1:2724220/93‑1 (MQ=255)

                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       <  1:662849/93‑1 (MQ=255)

                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       >  2:5107843/1‑93 (MQ=255)

                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       >  2:2344880/1‑93 (MQ=255)

                                   aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCTCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtc                                                      <  1:5298573/93‑1 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     <  2:2796067/93‑1 (MQ=255)

                                      gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCg                                                   <  1:5191531/93‑1 (MQ=255)

                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  2:2591167/93‑1 (MQ=255)

                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  2:294299/93‑1 (MQ=255)

                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  1:1120907/93‑1 (MQ=255)

                                         gTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCt                                                >  1:714623/1‑93 (MQ=255)

                                              aaCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAg                                           <  1:5661073/93‑1 (MQ=255)

                                               aCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgt                                          <  2:925058/93‑1 (MQ=255)

                                                ccGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtg                                         <  1:450735/93‑1 (MQ=255)

                                                 cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtgt                                        >  1:4981968/1‑93 (MQ=255)

                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       <  2:5185709/93‑1 (MQ=255)

                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       <  2:3301093/93‑1 (MQ=255)

                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       >  2:1459031/1‑93 (MQ=255)

                                                   ggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCt                                      <  2:407947/93‑1 (MQ=255)

                                                      gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGtt                                   <  2:5399648/93‑1 (MQ=255)

                                                        aaTGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTc                                 >  2:632729/1‑93 (MQ=255)

                                                        aaTGGTCCGTGCTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTc                                 <  1:4635874/93‑1 (MQ=255)

                                                         aTGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCt                                <  1:4041626/93‑1 (MQ=255)

                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTa                               <  1:2565700/93‑1 (MQ=255)

                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGACTGAAAGTGTCTGTTTCTa                               >  2:5988651/1‑93 (MQ=255)

                                                           tgTCCGTGTTAATGGTGGTCCGTTCGCTGACGTCAACGGTTTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAt                              <  1:3645670/92‑1 (MQ=255)

                                                            gTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATc                             <  1:3745443/93‑1 (MQ=255)

                                                             tCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCt                            <  1:3552279/93‑1 (MQ=255)

                                                               cGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  1:1775613/93‑1 (MQ=255)

                                                               cGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  1:6110084/93‑1 (MQ=255)

                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAGGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:4362142/1‑93 (MQ=255)

                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:1541102/1‑93 (MQ=255)

                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:3888845/1‑93 (MQ=255)

                                                                    tAATGATGGTCAGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCg                     <  1:4533998/93‑1 (MQ=255)

                                                                     aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGt                    >  2:2087645/1‑93 (MQ=255)

                                                                       tgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGc                  <  1:1963343/93‑1 (MQ=255)

                                                                       tgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGc                  >  1:2507927/1‑93 (MQ=255)

                                                                         atgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGa                >  2:5143033/1‑93 (MQ=255)

                                                                          tgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAc               <  1:4000214/93‑1 (MQ=255)

                                                                           ggcccGTTCGCTGACGTCCACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcc              >  1:3772795/4‑93 (MQ=255)

                                                                           ggTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcc              >  2:5888947/1‑93 (MQ=255)

                                                                            gTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAccc             <  2:3418710/93‑1 (MQ=255)

                                                                             tCCGTTCGCTGATGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcccc            <  1:3959115/93‑1 (MQ=255)

                                                                             tCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcccc            >  2:1149258/1‑93 (MQ=255)

                                                                               cGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCgg          >  1:3210826/1‑93 (MQ=255)

                                                                                gTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGt         <  2:3543241/93‑1 (MQ=255)

                                                                                 ttCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa        >  2:4913998/1‑93 (MQ=255)

                                                                                 ttCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa        >  1:4564257/1‑93 (MQ=255)

                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       <  2:3947406/93‑1 (MQ=255)

                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       >  2:5999172/1‑93 (MQ=255)

                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  1:5754962/93‑1 (MQ=255)

                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  1:5997138/93‑1 (MQ=255)

                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   <  1:2025334/93‑1 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  <  2:4865851/93‑1 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  2:4725615/1‑93 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  1:2632587/1‑93 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  1:915993/1‑93 (MQ=255)

CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`