BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,306	A→G	I417V (ATT→GTT)	yjcD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,306	0	A	G	93.0%	715.6 / 25.2	230	I417V (ATT→GTT)	yjcD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (12/4); new base G (102/112); total (114/116)
Fisher's exact test for biased strand distribution p-value = 4.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 248 total aligned reads displayed.

TGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTG  >  NC_000913/4277215‑4277397

 gATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGtt                                                                                           <  2:3680281/93‑1 (MQ=255)

 gATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGtt                                                                                           <  1:3998369/93‑1 (MQ=255)

  aTGCGATGGCGGGTCTGGTTACGTCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTg                                                                                          <  1:2001029/93‑1 (MQ=255)

  aTGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTg                                                                                          <  1:1667627/93‑1 (MQ=255)

    gCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGtggttggt                                                                                        >  2:227131/1‑93 (MQ=255)

    gCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGtggttggt                                                                                        >  2:4876462/1‑93 (MQ=255)

     cGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGgtc                                                                                       >  2:678038/1‑93 (MQ=255)

      gATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGgtcg                                                                                      <  1:4555784/93‑1 (MQ=255)

       aTGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTTgtcgt                                                                                     >  1:5149438/1‑93 (MQ=255)

        tGGCGGGTCTTGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGgtcgtc                                                                                    <  2:1243368/93‑1 (MQ=255)

         ggCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCt                                                                                   <  2:3812019/93‑1 (MQ=255)

           cGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTgg                                                                                 <  2:5530237/93‑1 (MQ=255)

            gggTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGt                                                                                <  1:5156330/93‑1 (MQ=255)

             ggTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGtt                                                                               >  2:1623782/1‑93 (MQ=255)

             ggTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGtt                                                                               <  1:108549/93‑1 (MQ=255)

               tCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTc                                                                             >  2:778261/1‑93 (MQ=255)

               tCTGGTTACAGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTc                                                                             >  1:3988771/1‑93 (MQ=255)

                  ggTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTcccg                                                                          >  1:1135862/1‑91 (MQ=255)

                  ggTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCgg                                                                          >  2:2281667/1‑93 (MQ=255)

                  ggTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCgg                                                                          >  1:1539666/1‑93 (MQ=255)

                   gTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGc                                                                         <  2:3377900/93‑1 (MQ=255)

                    ttACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCg                                                                        >  1:2866075/1‑93 (MQ=255)

                    ttACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCg                                                                        >  2:1097823/1‑93 (MQ=255)

                    ttACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCg                                                                        >  2:4206275/1‑93 (MQ=255)

                        ggCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATg                                                                    <  1:4874703/93‑1 (MQ=255)

                         gCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATgg                                                                   >  1:5315836/1‑93 (MQ=255)

                          cTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGgc                                                                  <  2:1002740/93‑1 (MQ=255)

                          cTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGgc                                                                  >  2:5431189/1‑93 (MQ=255)

                            gTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGgcgc                                                                >  2:6077818/1‑93 (MQ=255)

                            gTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGgagc                                                                >  2:3425379/1‑93 (MQ=255)

                             tATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCa                                                               >  2:1435316/1‑93 (MQ=255)

                             tATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCa                                                               <  2:5223048/93‑1 (MQ=255)

                              aTTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCaa                                                              >  2:5950988/1‑93 (MQ=255)

                              aTTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTAGTCTGGTTTCCGGCGAATGGCGCaa                                                              >  1:1859486/1‑93 (MQ=255)

                               ttCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAg                                                             >  2:2928691/1‑93 (MQ=255)

                               ttCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAg                                                             <  2:205435/93‑1 (MQ=255)

                               ttCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAg                                                             <  1:2331251/93‑1 (MQ=255)

                                tCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGt                                                            <  1:2076568/93‑1 (MQ=255)

                                 cATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGtt                                                           <  1:1586218/93‑1 (MQ=255)

                                 cATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGtt                                                           >  1:3319872/1‑93 (MQ=255)

                                  aTCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGtta                                                          >  1:364378/1‑92 (MQ=255)

                                  aTCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTg                                                          >  1:2997666/1‑93 (MQ=255)

                                   tCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGa                                                         >  2:6081237/1‑93 (MQ=255)

                                     gTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAAc                                                       <  2:1810429/93‑1 (MQ=255)

                                     gTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAAc                                                       >  1:1010015/1‑93 (MQ=255)

                                       gCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACAt                                                     <  1:5021573/93‑1 (MQ=255)

                                        cTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATc                                                    >  1:3753754/1‑93 (MQ=255)

                                         tGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCg                                                   >  2:3271804/1‑93 (MQ=255)

                                           aCCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGt                                                 <  2:4545398/93‑1 (MQ=255)

                                            ccTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTa                                                <  2:3908927/93‑1 (MQ=255)

                                              tGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACg                                              >  2:938875/1‑93 (MQ=255)

                                              tGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACg                                              >  1:1423224/1‑93 (MQ=255)

                                              tGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGGACg                                              >  1:4441541/1‑93 (MQ=255)

                                               gTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACgg                                             >  1:3849600/1‑93 (MQ=255)

                                               gTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACgg                                             <  2:3602459/93‑1 (MQ=255)

                                                 aaCATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAATTTGAACATCGGTACGGTc                                           <  2:2683959/93‑1 (MQ=255)

                                                 aaCATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTc                                           >  2:5511781/1‑93 (MQ=255)

                                                     tCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCGAGTTGAACATCGGTACGGGCGTTa                                       >  1:2365056/1‑93 (MQ=255)

                                                     tCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTAGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTa                                       >  2:515313/1‑93 (MQ=255)

                                                      cGTAACAGTCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTAt                                      <  1:3593757/93‑1 (MQ=255)

                                                      cGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTAt                                      <  1:4472462/93‑1 (MQ=255)

                                                      cGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTAt                                      <  1:5778321/93‑1 (MQ=255)

                                                      cGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTAt                                      <  2:2508488/93‑1 (MQ=255)

                                                       gTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATc                                     >  1:245264/1‑93 (MQ=255)

                                                         aaCAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGc                                   <  1:736704/93‑1 (MQ=255)

                                                         aaCAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGc                                   <  1:1698609/93‑1 (MQ=255)

                                                          aCNGCCANCATAATCGGCTTCNCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGcc                                  <  2:3817080/93‑1 (MQ=255)

                                                          aCAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGcc                                  >  1:4299265/1‑93 (MQ=255)

                                                          aCAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGcc                                  >  1:4685688/1‑93 (MQ=255)

                                                           cAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCg                                 <  1:1039390/93‑1 (MQ=255)

                                                           cAGGCATCATGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCg                                 <  2:1776449/93‑1 (MQ=255)

                                                               catcatGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGgc                             <  1:5905081/93‑1 (MQ=255)

                                                               catcatGAGCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGgc                             <  1:534918/93‑1 (MQ=255)

                                                                 tcatGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGgcgc                           <  1:5695181/93‑1 (MQ=255)

                                                                 tcatGATCGGCTTCGCGACTATGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGgcgc                           >  2:411510/1‑93 (MQ=255)

                                                                    tGATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTgg                        >  2:5617869/1‑93 (MQ=255)

                                                                     gATCGGCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTggg                       >  2:5655061/1‑92 (MQ=255)

                                                                      aTCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTgggg                      >  2:5062489/1‑91 (MQ=255)

                                                                       tCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGa                     >  1:5562614/1‑93 (MQ=255)

                                                                        cGGCTTCGCGACTCTTGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGAc                    <  1:1317387/93‑1 (MQ=255)

                                                                        cGGCTTCGCGACTCTGGTGGTTGGTCGGCTGGTTTCCGACGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGAc                    <  1:4096706/93‑1 (MQ=255)

                                                                         ggCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGAcc                   >  1:3115300/1‑93 (MQ=255)

                                                                          gCTTCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCt                  >  2:5010815/1‑93 (MQ=255)

                                                                             tCGCGACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCt               >  1:3808329/1‑93 (MQ=255)

                                                                              cgcgACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTa              <  2:614965/93‑1 (MQ=255)

                                                                              cgcgACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTa              <  2:3215416/93‑1 (MQ=255)

                                                                               gcgACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTAt             >  2:2607830/1‑93 (MQ=255)

                                                                                 gACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGc           >  2:1866135/1‑93 (MQ=255)

                                                                                 gACTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGc           <  2:211762/93‑1 (MQ=255)

                                                                                  aCTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCg          >  2:5862951/1‑93 (MQ=255)

                                                                                  aCTCTGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCg          >  2:1946416/1‑93 (MQ=255)

                                                                                  aCTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCg          <  1:2617474/93‑1 (MQ=255)

                                                                                   ctctGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCgg         <  1:3125481/93‑1 (MQ=255)

                                                                                    tctGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCggg        >  1:3235917/1‑93 (MQ=255)

                                                                                    tctGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCggg        >  2:4642634/1‑93 (MQ=255)

                                                                                    tctGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCCGGTGACCTTCTATGCggg        >  1:5413077/1‑93 (MQ=255)

                                                                                    tctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCggg        >  1:85109/1‑93 (MQ=255)

                                                                                     ctGGTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGt       <  1:622527/93‑1 (MQ=255)

                                                                                       ggtggtTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTgg     >  2:1725319/1‑93 (MQ=255)

                                                                                        gTGGTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGc    <  2:1347992/93‑1 (MQ=255)

TGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTG  >  NC_000913/4277215‑4277397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`