BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,236	T→C	L357S (TTG→TCG)	nagE →	PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,236	0	T	C	100.0%	640.8 / NA	177	L357S (TTG→TCG)	nagE	PTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (96/81); total (96/81)

Only 100 of 184 total aligned reads displayed.

TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704148‑704325

tataACCTGCCGGCCGCCAGCCAGACCGTCTGGATGCTGATGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  1:1162474/93‑1 (MQ=255)

tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  1:565546/93‑1 (MQ=255)

tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       >  1:4673064/1‑93 (MQ=255)

  taACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAt                                                                                     >  1:185169/1‑93 (MQ=255)

    aCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTCCTTCGTGGTGTTCAGTTCGG‑TATCCg                                                                                  <  2:4591183/93‑1 (MQ=255)

    aCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATcc                                                                                   >  2:4125103/1‑93 (MQ=255)

      cTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGc                                                                                 >  1:4115704/1‑93 (MQ=255)

        gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                               <  1:1238821/93‑1 (MQ=255)

          cggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGt                                                                             <  2:861437/93‑1 (MQ=255)

           ggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGtt                                                                            >  1:597858/1‑93 (MQ=255)

               cccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAc                                                                        <  2:310261/92‑1 (MQ=255)

                ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       <  1:5547994/93‑1 (MQ=255)

                ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       <  2:3952354/93‑1 (MQ=255)

                  agccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTg                                                                     <  1:3754621/93‑1 (MQ=255)

                   gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                    >  2:2323652/1‑93 (MQ=255)

                    ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   >  1:574490/1‑93 (MQ=255)

                    ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   <  1:5966170/93‑1 (MQ=255)

                     cagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGCGTTCAGTTCGGTTATCCGCATGTTCAACCTGacc                                                                  >  1:3222995/1‑91 (MQ=255)

                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:4481251/1‑93 (MQ=255)

                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  2:3875307/93‑1 (MQ=255)

                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  1:1010688/93‑1 (MQ=255)

                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  1:2695773/1‑93 (MQ=255)

                      agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  1:1698913/93‑1 (MQ=255)

                       gAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                >  2:1891776/1‑93 (MQ=255)

                       gAACGTCTGGATGCTGCTGGTGATGGGCGGTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                >  1:1411121/1‑93 (MQ=255)

                        aaCGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACg                                                               >  2:5545224/1‑93 (MQ=255)

                         aCGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGc                                                              <  1:951033/93‑1 (MQ=255)

                          cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                             <  1:667205/93‑1 (MQ=255)

                              tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGt                                                         <  2:4967275/93‑1 (MQ=255)

                               ggATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                        <  1:4823364/93‑1 (MQ=255)

                               ggATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                        <  1:4260253/93‑1 (MQ=255)

                               ggATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                        <  1:42135/93‑1 (MQ=255)

                                   gctgctGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    <  2:4021465/93‑1 (MQ=255)

                                   gctgctGGTGATGGGCCTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    <  2:1638591/93‑1 (MQ=255)

                                    ctgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGaa                                                   <  2:5658592/93‑1 (MQ=255)

                                     tgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                  >  2:5747130/1‑93 (MQ=255)

                                        tgGTGATGGTCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                               <  1:192492/93‑1 (MQ=255)

                                        tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                               <  2:3306707/93‑1 (MQ=255)

                                          gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaaa                                             <  2:1201780/93‑1 (MQ=255)

                                          gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaaa                                             >  2:5920488/1‑93 (MQ=255)

                                            gATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaaga                                           <  1:3609230/93‑1 (MQ=255)

                                             aTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaa                                          >  1:3929054/1‑93 (MQ=255)

                                             aTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaa                                          >  2:3584208/1‑93 (MQ=255)

                                              ttggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         <  2:2243501/91‑1 (MQ=255)

                                              tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         >  2:3697555/1‑93 (MQ=255)

                                                ggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAc                                       >  2:2991778/1‑93 (MQ=255)

                                                   gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgag                                    >  2:5025471/1‑93 (MQ=255)

                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   >  1:3460147/1‑93 (MQ=255)

                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   <  2:5276377/93‑1 (MQ=255)

                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   >  1:5299588/1‑93 (MQ=255)

                                                    ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   <  1:4905639/93‑1 (MQ=255)

                                                      aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 >  2:6056902/1‑93 (MQ=255)

                                                      aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 >  2:533117/1‑93 (MQ=255)

                                                      aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 <  1:5045825/93‑1 (MQ=255)

                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                <  1:3783619/93‑1 (MQ=255)

                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                >  2:5273613/1‑93 (MQ=255)

                                                       tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                >  2:2007747/1‑93 (MQ=255)

                                                        cttcttCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGt                               >  2:5193854/1‑93 (MQ=255)

                                                        cttcttCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGt                               <  2:1035611/93‑1 (MQ=255)

                                                         ttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGtt                              <  1:4213073/93‑1 (MQ=255)

                                                               gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        >  2:785503/1‑93 (MQ=255)

                                                               gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        >  2:2070722/1‑93 (MQ=255)

                                                                cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       >  2:2715728/1‑93 (MQ=255)

                                                                cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       >  1:3570939/1‑93 (MQ=255)

                                                                 tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                      >  1:2276918/1‑93 (MQ=255)

                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  2:4362958/1‑93 (MQ=255)

                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  2:461181/1‑93 (MQ=255)

                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  2:537593/1‑93 (MQ=255)

                                                                  aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaagaa                     >  1:1288540/1‑93 (MQ=255)

                                                                   tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                    >  1:1311032/1‑93 (MQ=255)

                                                                    ttACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                   <  2:20753/92‑1 (MQ=255)

                                                                    cTACTTTGTGGTGTTCAGTTCGGTTACCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                   <  1:3953348/93‑1 (MQ=255)

                                                                     tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGcc                  >  2:5899968/1‑93 (MQ=255)

                                                                     tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGcc                  >  1:2803135/1‑93 (MQ=255)

                                                                     tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGca                  >  1:3650095/1‑92 (MQ=255)

                                                                      aCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCa                 >  1:5876126/1‑93 (MQ=255)

                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGTTACTGAAGAAGCCAAc               >  1:5182733/1‑93 (MQ=255)

                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc               >  2:5243422/1‑93 (MQ=255)

                                                                        ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc               <  1:2391007/93‑1 (MQ=255)

                                                                         tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACa              <  1:4040520/93‑1 (MQ=255)

                                                                          cGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg             <  2:5976916/93‑1 (MQ=255)

                                                                           gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCTGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGc            <  2:1608442/93‑1 (MQ=255)

                                                                           gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGc            >  1:1104194/1‑93 (MQ=255)

                                                                           gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGc            >  1:2585705/1‑93 (MQ=255)

                                                                            tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCa           >  1:4886561/1‑93 (MQ=255)

                                                                              gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         <  2:3627593/93‑1 (MQ=255)

                                                                              gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         >  2:2849398/1‑93 (MQ=255)

                                                                               tgtTCATTTCGGTGATCCGCATGTTCAACCGGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        <  2:106758/93‑1 (MQ=255)

                                                                               tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        <  1:5088476/93‑1 (MQ=255)

                                                                               tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGAGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        <  1:802049/93‑1 (MQ=255)

                                                                                gtgcAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCATTACTGAAGAAGCCAACAGCAacac       <  1:175593/90‑1 (MQ=255)

                                                                                gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAacac       <  2:1278168/93‑1 (MQ=255)

                                                                                 ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt      <  1:5339487/93‑1 (MQ=255)

                                                                                  tCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTg     >  2:4008979/1‑93 (MQ=255)

                                                                                  tCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTg     >  1:2305446/1‑93 (MQ=255)

                                                                                    aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaa   >  1:3048367/1‑93 (MQ=255)

                                                                                    aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaa   >  2:3263822/1‑93 (MQ=255)

                                                                                    aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAAAGCAACACTgaa   >  1:1431864/1‑93 (MQ=255)

                                                                                    aGTTCGGGTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaa   >  1:3048368/1‑93 (MQ=255)

                                                                                     gTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaag  <  1:636871/93‑1 (MQ=255)

TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704148‑704325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`