BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,170	T→C	I502I (ATT→ATC)	fimD →	FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,170	0	T	C	100.0%	1029.2 / NA	281	I502I (ATT→ATC)	fimD	FimD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (149/132); total (149/132)

Only 100 of 286 total aligned reads displayed.

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  2:2718947/1‑93 (MQ=255)

  atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaaa                                                                                       <  2:4579171/93‑1 (MQ=255)

  atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaaa                                                                                       <  2:1815486/93‑1 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      <  1:3126008/93‑1 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      >  1:1013802/1‑93 (MQ=255)

    atTCAGTTAGTGTGTTTCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAaca                                                                                     <  1:4979293/93‑1 (MQ=255)

    atTCAGTTAGTGGGTTACCTTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAaca                                                                                     <  2:785620/93‑1 (MQ=255)

        aGTTAGTGGGGTACCGTTATGCGACCAGCGTATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAgg                                                                                 <  1:5808919/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  2:3815211/1‑93 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                <  2:3748108/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                <  1:5376656/93‑1 (MQ=255)

           tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACg                                                                              <  1:3336193/93‑1 (MQ=255)

           tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACg                                                                              <  1:1439368/93‑1 (MQ=255)

            aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACgg                                                                             >  2:3360775/1‑93 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  1:5056544/93‑1 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:4088781/93‑1 (MQ=255)

                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  2:1103923/93‑1 (MQ=255)

                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  2:4276360/93‑1 (MQ=255)

                    aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTc                                                                     <  2:3254665/93‑1 (MQ=255)

                     ccGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCa                                                                    <  2:1543099/93‑1 (MQ=255)

                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAgg                                                                  >  2:4668220/1‑93 (MQ=255)

                        ttATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGt                                                                 >  1:5531867/1‑93 (MQ=255)

                        ttATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGt                                                                 >  2:5265063/1‑93 (MQ=255)

                         tATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGCtt                                                                >  1:4292957/1‑93 (MQ=255)

                          aTTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTa                                                               >  1:2836682/1‑93 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              <  2:5106587/93‑1 (MQ=255)

                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             <  2:2308375/93‑1 (MQ=255)

                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             <  2:207755/93‑1 (MQ=255)

                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             >  1:422876/1‑93 (MQ=255)

                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             <  1:4379484/93‑1 (MQ=255)

                              gACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGcc                                                           >  2:1901697/1‑93 (MQ=255)

                              gACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGcc                                                           >  2:60302/1‑93 (MQ=255)

                              gACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGcc                                                           >  1:3271062/1‑93 (MQ=255)

                                ccAGCTGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGa                                                         <  1:4640935/93‑1 (MQ=255)

                                ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGa                                                         <  1:5515320/93‑1 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       >  2:4213507/1‑93 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGACACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       <  2:2362872/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      >  1:4767203/1‑93 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      <  1:2040783/93‑1 (MQ=255)

                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    >  2:5707066/1‑93 (MQ=255)

                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    <  1:4901556/93‑1 (MQ=255)

                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  1:3468167/1‑93 (MQ=255)

                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   <  2:5820360/93‑1 (MQ=255)

                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCCACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  1:757673/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  2:1740400/93‑1 (MQ=255)

                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGGTATTCAGGTTAAGACGAAATTCAcc                                                 >  1:2537468/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:434386/93‑1 (MQ=255)

                                            ttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACt                                             >  1:448825/1‑93 (MQ=255)

                                             tAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTa                                            >  2:1207153/1‑93 (MQ=255)

                                               aTTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAtt                                          <  1:958214/93‑1 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGCGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  1:3043409/1‑93 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  2:2816022/1‑93 (MQ=255)

                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        >  2:9273/1‑93 (MQ=255)

                                                  tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGCGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACa                                       >  1:3815096/1‑93 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  2:2562346/93‑1 (MQ=255)

                                                     cTGATACAACATGCAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                    >  1:3646165/1‑93 (MQ=255)

                                                     cTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                    <  2:5907622/93‑1 (MQ=255)

                                                     cTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                    <  1:780248/93‑1 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   >  1:2144273/1‑93 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   >  2:5021077/1‑93 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  >  1:3849421/1‑93 (MQ=255)

                                                        aTACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCg                                 >  1:4107256/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  2:3888601/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  2:5798246/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  2:1508851/1‑93 (MQ=255)

                                                           caacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCtt                              <  1:2355418/93‑1 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:168650/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  2:2598729/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  2:3074505/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  2:1456749/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  1:6061161/1‑93 (MQ=255)

                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           >  1:1858045/1‑93 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          >  1:5305410/1‑93 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          <  2:3673057/93‑1 (MQ=255)

                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       <  2:4590141/93‑1 (MQ=255)

                                                                      cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   >  1:2614918/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:1393244/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:1252433/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  1:3973858/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  1:189424/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               <  2:5549356/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  2:1642334/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             >  2:1035024/1‑93 (MQ=255)

                                                                             atgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAt            <  2:5449029/93‑1 (MQ=255)

                                                                               ggCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTa          <  2:480378/93‑1 (MQ=255)

                                                                                gccacaacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  1:5186579/90‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTaa         >  2:4414627/1‑92 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:3553420/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  1:352996/93‑1 (MQ=255)

                                                                                 cTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACa        >  2:5512775/1‑93 (MQ=255)

                                                                                  tACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa       >  1:4920237/1‑93 (MQ=255)

                                                                                  tACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa       >  1:3092237/1‑93 (MQ=255)

                                                                                  tACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa       >  2:644780/1‑93 (MQ=255)

                                                                                  tACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa       >  2:2031702/1‑93 (MQ=255)

                                                                                   acaacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAGATTACAAc      >  2:5891985/1‑93 (MQ=255)

                                                                                   acaacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAc      >  2:5200570/1‑93 (MQ=255)

                                                                                   acaacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAc      >  1:2436140/1‑93 (MQ=255)

                                                                                    caacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACt     <  2:4756744/93‑1 (MQ=255)

                                                                                    caacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACt     >  1:1095333/1‑93 (MQ=255)

                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCa   >  2:5928166/1‑93 (MQ=255)

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`