BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	764,635	T→C	Q155R (CAG→CGG)	farR ←	FarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	764,635	0	T	C	94.5%	700.7 / 19.9	218	Q155R (CAG→CGG)	farR	FarR
Reads supporting (aligned to +/- strand): ref base T (8/4); new base C (129/77); total (137/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 223 total aligned reads displayed.

GGCATCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCTTCACA  >  NC_000913/764545‑764724

   aTCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAg                                                                                      <  2:1698686/93‑1 (MQ=255)

     cggagGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGt                                                                                    <  1:5147810/91‑1 (MQ=255)

     cAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGt                                                                                    >  2:5081379/1‑93 (MQ=255)

     cAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGt                                                                                    >  1:5084074/1‑93 (MQ=255)

       gagGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTga                                                                                  <  2:215282/93‑1 (MQ=255)

        agGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTgag                                                                                 >  2:1539716/1‑93 (MQ=255)

         ggAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTgaga                                                                                <  2:2753299/93‑1 (MQ=255)

          gAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGAt                                                                               >  1:5060555/1‑93 (MQ=255)

          gAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGAt                                                                               >  2:2087295/1‑93 (MQ=255)

          gAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGAGAGAt                                                                               >  2:703642/1‑93 (MQ=255)

           aaTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATc                                                                              >  2:309504/1‑93 (MQ=255)

           aaTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATc                                                                              <  1:2059080/93‑1 (MQ=255)

           aaTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATc                                                                              >  1:5521802/1‑93 (MQ=255)

           aaTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATc                                                                              >  1:3834967/1‑93 (MQ=255)

            aTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATcc                                                                             >  2:1041606/1‑93 (MQ=255)

            aTGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATcc                                                                             <  1:5879129/93‑1 (MQ=255)

             tGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCg                                                                            >  2:5162083/1‑93 (MQ=255)

             tGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCg                                                                            <  1:784686/93‑1 (MQ=255)

              gATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCgg                                                                           >  1:4870238/1‑93 (MQ=255)

              gATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCgg                                                                           <  2:2229668/93‑1 (MQ=255)

                tttCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGa                                                                         >  1:1112539/1‑93 (MQ=255)

                 ttCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaa                                                                        >  2:2696436/1‑93 (MQ=255)

                  tCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaac                                                                       >  1:2379206/1‑93 (MQ=255)

                  tCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaac                                                                       >  2:5252276/1‑93 (MQ=255)

                   tcTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaaca                                                                      <  2:1498584/92‑1 (MQ=255)

                    cTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaacaa                                                                     <  2:3140053/93‑1 (MQ=255)

                    cTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaacaa                                                                     >  1:130155/1‑93 (MQ=255)

                    cTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaacaa                                                                     >  1:3297025/1‑93 (MQ=255)

                    cTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaacaa                                                                     >  2:5546059/1‑93 (MQ=255)

                     tGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGaacaac                                                                    <  2:5952776/93‑1 (MQ=255)

                          gCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAg                                                               >  2:1286516/1‑93 (MQ=255)

                          gCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAg                                                               >  1:973947/1‑93 (MQ=255)

                           cTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGc                                                              <  1:4353332/93‑1 (MQ=255)

                           cTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGc                                                              <  2:1958278/93‑1 (MQ=255)

                           cTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGc                                                              <  2:485361/93‑1 (MQ=255)

                           cTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGc                                                              <  1:1041215/93‑1 (MQ=255)

                            tACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCg                                                             >  1:4612895/1‑93 (MQ=255)

                            tACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCg                                                             >  1:1886810/1‑93 (MQ=255)

                             tcgatcgatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCgg                                                            <  2:3266139/92‑1 (MQ=255)

                                atcgatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCAt                                                         >  2:2669330/1‑93 (MQ=255)

                                atcgatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCAt                                                         >  1:4830827/1‑93 (MQ=255)

                                  cgatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATcc                                                       >  1:3242181/1‑93 (MQ=255)

                                   gatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCa                                                      <  2:3558059/93‑1 (MQ=255)

                                   gatAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCa                                                      >  1:5114871/1‑93 (MQ=255)

                                     tAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAgg                                                    >  2:2275470/1‑93 (MQ=255)

                                     tAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAgg                                                    >  2:5908203/1‑93 (MQ=255)

                                     tAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAgg                                                    >  2:5427600/1‑93 (MQ=255)

                                     tAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAgg                                                    >  2:1914800/1‑93 (MQ=255)

                                     tAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAgg                                                    >  1:3946573/1‑93 (MQ=255)

                                      aaCCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGt                                                   >  2:5325753/1‑93 (MQ=255)

                                       aCCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGtt                                                  >  2:3237236/1‑93 (MQ=255)

                                         cATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTttc                                                >  2:1413679/1‑93 (MQ=255)

                                          aTTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTttct                                               >  1:3803702/1‑93 (MQ=255)

                                          aTTTTCTTCACTTCTTCGATAAACTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTttct                                               <  1:3492672/93‑1 (MQ=255)

                                              tcttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCa                                           >  1:4157019/1‑93 (MQ=255)

                                               cttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAg                                          >  2:3803371/1‑93 (MQ=255)

                                                ttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAgc                                         >  2:5235077/1‑93 (MQ=255)

                                                ttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAgc                                         <  2:5867478/93‑1 (MQ=255)

                                                ttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTATTCAAga                                         >  1:2835470/1‑92 (MQ=255)

                                                ttcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGCGAGATCCGGGAACAACGCAAGCGGCATCCAGGATTCTTCCAgc                                         >  1:791353/1‑93 (MQ=255)

                                                 tcACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAgcg                                        >  1:6019260/1‑93 (MQ=255)

                                                  cACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAgcgc                                       <  2:3087292/93‑1 (MQ=255)

                                                   aCTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGcc                                      >  2:5776336/1‑93 (MQ=255)

                                                     ttcttcGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTGAGATCCGGGAACAACACAAGCGGCATCCAGGTTTCTTCCAGCGCCAt                                    >  1:5335571/1‑93 (MQ=255)

                                                     ttcttcGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCAt                                    >  2:1868312/1‑93 (MQ=255)

                                                     ttcttcGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGCTTCTTCCAGCGCCAt                                    >  2:3368774/1‑93 (MQ=255)

                                                      tcttcGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCAtt                                   <  2:2011613/93‑1 (MQ=255)

                                                      tcttcGATAAAGTGATATTTCGAATTTTCCATGACCCGCCACGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTCATTCCAGCGCCAtg                                   >  2:2347940/1‑92 (MQ=255)

                                                       cttcGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTg                                  <  2:4783794/93‑1 (MQ=255)

                                                          cGATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCt                               <  2:4318490/93‑1 (MQ=255)

                                                           gATAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCtt                              <  1:2112585/93‑1 (MQ=255)

                                                            aTAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCttt                             >  1:5402522/1‑93 (MQ=255)

                                                            aTAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCttt                             <  1:2658959/93‑1 (MQ=255)

                                                             tAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCtttt                            >  1:1468929/1‑93 (MQ=255)

                                                             tAAAGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCtttt                            >  1:1183759/1‑93 (MQ=255)

                                                              aaaGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttg                           >  2:775755/1‑93 (MQ=255)

                                                               aaGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgc                          >  2:1815590/1‑93 (MQ=255)

                                                               aaGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgc                          >  2:4026113/1‑93 (MQ=255)

                                                               aaGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgc                          >  2:3836163/1‑93 (MQ=255)

                                                                aGTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgct                         <  2:2918374/93‑1 (MQ=255)

                                                                 gTGATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgctt                        >  1:2895796/1‑93 (MQ=255)

                                                                   gATATTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTttgcttgc                      >  2:751229/1‑93 (MQ=255)

                                                                    atatTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCg                     >  1:4218405/1‑93 (MQ=255)

                                                                     tatTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGa                    <  1:2746170/93‑1 (MQ=255)

                                                                      atTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGAt                   <  2:1228084/93‑1 (MQ=255)

                                                                      atTTCGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGAt                   <  1:3759152/93‑1 (MQ=255)

                                                                          cGAATTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAgcg               <  1:4981314/93‑1 (MQ=255)

                                                                            aaTTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCa             <  2:3631962/93‑1 (MQ=255)

                                                                            aaTTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCa             <  2:1152083/93‑1 (MQ=255)

                                                                             aTTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCAc            <  2:4569872/93‑1 (MQ=255)

                                                                             aTTTTCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCAc            >  1:944140/1‑93 (MQ=255)

                                                                              ttttCCATGACCTGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCAcg           <  1:2569565/93‑1 (MQ=255)

                                                                              ttttCCATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCAcg           >  1:3654392/1‑93 (MQ=255)

                                                                                  ccATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCt       <  2:4952829/93‑1 (MQ=255)

                                                                                   cATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCtt      >  1:614557/1‑93 (MQ=255)

                                                                                   cATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCtt      >  1:3285255/1‑93 (MQ=255)

                                                                                   cATGACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCtt      >  2:3387049/1‑93 (MQ=255)

                                                                                      gACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCTTcac   >  1:3783204/1‑93 (MQ=255)

                                                                                      gACCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCTTcac   >  2:4802613/1‑93 (MQ=255)

                                                                                       aCCCGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCTTcaca  <  2:907782/93‑1 (MQ=255)

GGCATCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAATTTTCCATGACCTGCCAGGTGAGATCCGGGAACAACGCAAGCGGCATCCAGGTTTCTTCCAGCGCCATTGGCTTTTGCTTGCGATAGCGCACGCGCTTCACA  >  NC_000913/764545‑764724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`