BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,851	C→T	V9I (GTT→ATT)	arcA ←	ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,851	0	C	T	95.7%	999.8 / 17.5	279	V9I (GTT→ATT)	arcA	ArcA
Reads supporting (aligned to +/- strand): ref base C (8/4); new base T (159/108); total (167/112)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

Only 100 of 284 total aligned reads displayed.

CATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATG  >  NC_000913/4637759‑4637940

  tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGa                                                                                         >  1:3336060/1‑93 (MQ=255)

  tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGa                                                                                         >  2:6007594/1‑93 (MQ=255)

  tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGa                                                                                         <  2:1660565/93‑1 (MQ=255)

  tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa                                                                                         >  2:6098905/1‑93 (MQ=255)

     ccGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATaa                                                                                      >  1:947602/1‑93 (MQ=255)

      cgcgCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAg                                                                                     <  1:450418/93‑1 (MQ=255)

          ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATTCTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAAtg                                                                                 <  2:3563594/93‑1 (MQ=255)

          ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAAtg                                                                                 <  2:1170480/93‑1 (MQ=255)

          ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAAtg                                                                                 >  1:3682116/1‑93 (MQ=255)

            aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAAtgtg                                                                               >  1:5857239/1‑93 (MQ=255)

             tCTGTCTCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGc                                                                              <  1:1837688/93‑1 (MQ=255)

             tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGc                                                                              >  1:4516837/1‑93 (MQ=255)

             tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGc                                                                              >  2:2572663/1‑93 (MQ=255)

              cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCATTGATAAGAATGTGCg                                                                             >  2:5821354/1‑93 (MQ=255)

              cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCg                                                                             <  2:1566843/93‑1 (MQ=255)

              cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCg                                                                             <  1:6121229/93‑1 (MQ=255)

                gTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCggg                                                                           >  1:5997199/1‑93 (MQ=255)

                gTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCggg                                                                           <  2:818602/93‑1 (MQ=255)

                 tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCgggg                                                                          >  2:2999675/1‑93 (MQ=255)

                 tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCgggg                                                                          >  1:4352580/1‑93 (MQ=255)

                   gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTc                                                                        <  1:2231831/93‑1 (MQ=255)

                   gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTc                                                                        >  2:1279790/1‑93 (MQ=255)

                   gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTc                                                                        <  2:5515534/93‑1 (MQ=255)

                   gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTc                                                                        <  1:4189387/93‑1 (MQ=255)

                    cTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGGTGCGTGTTACCAACTCGTCTTTAACGATATGAATGTGCGGGGTcg                                                                       >  2:5458595/1‑92 (MQ=255)

                     ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTg                                                                      >  1:4157680/1‑93 (MQ=255)

                       cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCa                                                                    <  1:3307164/93‑1 (MQ=255)

                         aaaaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATg                                                                  <  1:3148766/93‑1 (MQ=255)

                          aaaCATCATAGCATTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTGCCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGt                                                                 <  1:2623586/93‑1 (MQ=255)

                           aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGtt                                                                >  2:338699/1‑93 (MQ=255)

                            aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGttt                                                               <  2:1544163/93‑1 (MQ=255)

                                 atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTAc                                                          <  2:4574005/93‑1 (MQ=255)

                                 atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTAc                                                          >  2:4991083/1‑93 (MQ=255)

                                  tAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTAcc                                                         >  2:3810802/1‑93 (MQ=255)

                                  tAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTAcc                                                         >  1:1061459/1‑93 (MQ=255)

                                  tAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTAcc                                                         <  1:2363063/93‑1 (MQ=255)

                                    gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa                                                       >  1:6095011/1‑93 (MQ=255)

                                      cTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTaaa                                                     >  1:5719394/1‑93 (MQ=255)

                                       ttCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                                    >  1:4435005/1‑93 (MQ=255)

                                        tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAtt                                                   <  2:5800466/93‑1 (MQ=255)

                                        tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAtt                                                   <  1:4983922/93‑1 (MQ=255)

                                        tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAtt                                                   >  1:1509330/1‑93 (MQ=255)

                                         ccGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTg                                                  <  2:4102422/93‑1 (MQ=255)

                                          cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGc                                                 <  2:1386143/93‑1 (MQ=255)

                                          cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGc                                                 >  2:4590440/1‑93 (MQ=255)

                                            cTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCa                                               >  1:2656620/1‑93 (MQ=255)

                                            cTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCa                                               <  2:3951874/93‑1 (MQ=255)

                                             ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCaa                                              >  1:4619897/1‑93 (MQ=255)

                                             ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCaa                                              >  2:2672164/1‑93 (MQ=255)

                                                gAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTa                                           >  2:4196177/1‑93 (MQ=255)

                                                gAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTa                                           >  1:2531955/1‑93 (MQ=255)

                                                 aaaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                          >  2:3727988/1‑93 (MQ=255)

                                                 aaaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                          >  1:5165514/1‑93 (MQ=255)

                                                 aaaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                          >  1:2487790/1‑93 (MQ=255)

                                                  aaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTTTGCATGTTTGCTATCTAAATTGCCTACTaaa                                         >  1:5491747/1‑93 (MQ=255)

                                                   aaTACTTTTCAACGTGTTGCGTGTTTCCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAAt                                        <  2:5279977/93‑1 (MQ=255)

                                                   aaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAAt                                        <  2:1483591/93‑1 (MQ=255)

                                                   aaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAAt                                        <  2:5572678/93‑1 (MQ=255)

                                                    aTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATc                                       >  1:3895056/1‑93 (MQ=255)

                                                      aCTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGa                                     >  1:1978059/1‑93 (MQ=255)

                                                      aCTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGa                                     >  1:5350285/1‑93 (MQ=255)

                                                       cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaa                                    <  1:1258729/93‑1 (MQ=255)

                                                        ttttCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                                   >  1:5153442/1‑93 (MQ=255)

                                                        ttttCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                                   <  1:1453418/93‑1 (MQ=255)

                                                        ttttCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                                   >  2:4429055/1‑93 (MQ=255)

                                                           tCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAg                                <  2:4292134/93‑1 (MQ=255)

                                                           tCAACGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAg                                <  1:4077952/93‑1 (MQ=255)

                                                             aaCGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGa                              <  1:2323305/93‑1 (MQ=255)

                                                              aCGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGaa                             >  2:1437643/1‑93 (MQ=255)

                                                               cGTGTTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAg                            >  1:4773898/1‑93 (MQ=255)

                                                                gtgtTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGt                           <  1:1734866/93‑1 (MQ=255)

                                                                 tgtTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTa                          >  1:1363285/1‑93 (MQ=255)

                                                                  gtTGCGTGTTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTAc                         >  1:5421598/1‑93 (MQ=255)

                                                                   tagCGTGTTACCAGCTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACa                        <  2:1089425/91‑1 (MQ=255)

                                                                        tgtTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAgc                   >  2:1989775/1‑92 (MQ=255)

                                                                        tgtTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGt                   >  2:2359586/1‑93 (MQ=255)

                                                                         gtTACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTc                  >  1:2165517/1‑93 (MQ=255)

                                                                          ttACCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTcc                 >  2:4896202/1‑93 (MQ=255)

                                                                            tccAACTTGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCt               <  2:4497825/92‑1 (MQ=255)

                                                                            aCCAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCt               <  1:1174060/93‑1 (MQ=255)

                                                                             ccAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTg              >  1:1005528/1‑93 (MQ=255)

                                                                             ccAACTCGTCTTCAATGATAAGAATGTGCGGGCTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAACGTCCCTg              >  1:5024708/1‑93 (MQ=255)

                                                                              cAACTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa             <  2:1586118/93‑1 (MQ=255)

                                                                              cAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa             >  1:290711/1‑93 (MQ=255)

                                                                                aCTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcc           <  1:2800267/93‑1 (MQ=255)

                                                                                 cTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcct          >  2:3203720/1‑93 (MQ=255)

                                                                                 cTCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcct          >  1:423980/1‑93 (MQ=255)

                                                                                  tCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctg         >  2:473376/1‑93 (MQ=255)

                                                                                  tCGTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctg         >  2:3640299/1‑93 (MQ=255)

                                                                                   cGTCTTCAATGATAAGAATGTGCGGGTTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgc        <  1:2705085/93‑1 (MQ=255)

                                                                                    gTCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcc       >  1:1107642/1‑93 (MQ=255)

                                                                                     tCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCACCTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcct      >  1:307471/1‑93 (MQ=255)

                                                                                     tCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcct      >  2:483332/1‑93 (MQ=255)

                                                                                     tCTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcct      >  2:2748859/1‑93 (MQ=255)

                                                                                      cTTCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcctg     <  2:3312472/93‑1 (MQ=255)

                                                                                       ttCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGa    <  1:3623495/93‑1 (MQ=255)

                                                                                       ttCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGa    >  2:2195682/1‑93 (MQ=255)

                                                                                        tCAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGat   <  2:645501/93‑1 (MQ=255)

                                                                                         cAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatg  >  1:482329/1‑93 (MQ=255)

                                                                                         cAATGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatg  <  1:1537311/93‑1 (MQ=255)

CATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATG  >  NC_000913/4637759‑4637940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`