BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,370	T→C	E28E (GAA→GAG)	yeaA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,370	0	T	C	99.5%	710.2 / NA	198	E28E (GAA→GAG)	yeaA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (101/96); minor base G (1/0); total (102/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

 gTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGctct                                                                                       >  1:3547202/1‑93 (MQ=255)

   ttGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGt                                                                                     >  1:996758/1‑93 (MQ=255)

   ttGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGt                                                                                     >  1:263623/1‑93 (MQ=255)

    tGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTc                                                                                    >  2:6090303/1‑93 (MQ=255)

      ggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTccc                                                                                  >  2:3021784/1‑93 (MQ=255)

       gAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCa                                                                                 >  1:5366169/1‑93 (MQ=255)

        aaTGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCAt                                                                                <  2:2397637/93‑1 (MQ=255)

          tGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGa                                                                              >  1:924221/1‑93 (MQ=255)

          tGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGa                                                                              <  2:4142404/93‑1 (MQ=255)

            aaaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGAtt                                                                            >  2:473682/1‑93 (MQ=255)

             aaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTc                                                                           <  2:3166435/93‑1 (MQ=255)

              aCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCt                                                                          <  1:1466395/93‑1 (MQ=255)

              aCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCt                                                                          >  2:4787441/1‑93 (MQ=255)

              aCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCt                                                                          >  2:1784500/1‑93 (MQ=255)

               cAGCGGGGGCTCACAGGTCAAACAGTGATTTGCGCCGTCACGGTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTg                                                                         <  2:1842953/93‑1 (MQ=255)

                aGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGc                                                                        <  1:2254496/93‑1 (MQ=255)

                    gggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCa                                                                    >  2:5758413/1‑93 (MQ=255)

                    gggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCa                                                                    >  2:2674370/1‑93 (MQ=255)

                       cATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGt                                                                 <  2:5997734/93‑1 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  2:2004434/1‑93 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  2:2569627/1‑93 (MQ=255)

                         tCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                                               >  2:1049825/1‑93 (MQ=255)

                         tCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                                               >  2:3300479/1‑93 (MQ=255)

                           gCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaaa                                                             <  2:4016107/93‑1 (MQ=255)

                           gCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaaa                                                             >  1:5189407/1‑93 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            <  1:1673481/93‑1 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            >  2:5030017/1‑93 (MQ=255)

                            cAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAc                                                            <  2:1377044/93‑1 (MQ=255)

                             aGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACt                                                           >  1:5264142/1‑93 (MQ=255)

                                tCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCa                                                        >  1:3102052/1‑93 (MQ=255)

                                  aaaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtc                                                      <  1:4057053/93‑1 (MQ=255)

                                  aaaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtc                                                      <  2:177409/93‑1 (MQ=255)

                                  aaaCAGTGATATACGCCGTCACGCTTGTTATGCAGGAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAAATGCAtt                                                      >  1:1899812/1‑92 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     >  2:908237/1‑93 (MQ=255)

                                     cAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                                   <  2:4200787/93‑1 (MQ=255)

                                     cAGTGATATACGCCGTCACGCNTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                                   <  2:5146740/93‑1 (MQ=255)

                                      aGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCgg                                                  >  2:6090477/1‑93 (MQ=255)

                                      aGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCgg                                                  <  2:1585150/93‑1 (MQ=255)

                                       gTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 <  2:1354137/93‑1 (MQ=255)

                                       gTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 <  1:5043927/93‑1 (MQ=255)

                                       gTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 <  2:5385143/93‑1 (MQ=255)

                                       gTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 <  2:5553379/93‑1 (MQ=255)

                                         gATATACGCCGTCACTGTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACa                                               <  2:4701077/93‑1 (MQ=255)

                                           tataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGCCaaa                                             >  1:793010/1‑93 (MQ=255)

                                           tataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaat                                             >  2:1160219/1‑92 (MQ=255)

                                           tataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaaa                                             <  1:669529/93‑1 (MQ=255)

                                           tataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaaa                                             >  1:2616389/1‑93 (MQ=255)

                                            ataCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAt                                            >  1:4516814/1‑93 (MQ=255)

                                             taCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtt                                           >  1:5917122/1‑93 (MQ=255)

                                             taCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtt                                           >  2:272949/1‑93 (MQ=255)

                                              aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttt                                          >  1:3567765/1‑93 (MQ=255)

                                                gCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttttt                                        <  1:155341/93‑1 (MQ=255)

                                                 ccGTCACGCTTGTTATGCAGTAAACGACCCGTCAATGGCGGCTCTTTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                       >  1:4228676/1‑93 (MQ=255)

                                                 ccGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                       <  1:2531203/93‑1 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     >  1:708231/1‑93 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     >  2:389190/1‑93 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     >  1:3398599/1‑93 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     >  2:4891994/1‑93 (MQ=255)

                                                    tCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                                    >  1:5783305/1‑93 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   >  2:4992341/1‑93 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGCCCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGCCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                <  1:4564783/93‑1 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                >  1:2756876/1‑93 (MQ=255)

                                                         cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGTGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc                               <  1:3269992/93‑1 (MQ=255)

                                                         cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc                               <  2:3612731/93‑1 (MQ=255)

                                                           tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttctt                             <  1:2317893/93‑1 (MQ=255)

                                                           tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttctt                             >  1:122076/1‑93 (MQ=255)

                                                           tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTAAGttctt                             >  2:2429401/1‑93 (MQ=255)

                                                            tttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  2:65140/92‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  2:2778194/93‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  1:4968035/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  2:723629/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  1:197075/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  2:326672/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  1:2414251/93‑1 (MQ=255)

                                                              tATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg                          >  2:1265395/1‑93 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        >  2:2265870/1‑93 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        <  1:5159694/93‑1 (MQ=255)

                                                                tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                        >  2:4643296/1‑93 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTACAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCCGCCGaa                     >  2:1640886/1‑93 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     >  2:1812670/1‑93 (MQ=255)

                                                                   aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     <  1:2909493/93‑1 (MQ=255)

                                                                     tAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAgg                   >  2:4187435/1‑93 (MQ=255)

                                                                      aaaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGt                  >  2:1647756/1‑93 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  1:6131273/93‑1 (MQ=255)

                                                                        aCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGttt                >  2:220904/1‑93 (MQ=255)

                                                                          gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttat              >  1:3787306/1‑93 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           <  1:1208237/93‑1 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTAAGTTCTTCTGCCGAAGGTTTATTAg           >  2:1953995/1‑93 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          >  1:2930756/1‑93 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          >  1:5350850/1‑93 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          <  2:1075537/93‑1 (MQ=255)

                                                                               gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGGCGGAGGGTTATTAGcc         >  2:5952445/1‑93 (MQ=255)

                                                                               gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGcc         >  2:4466373/1‑93 (MQ=255)

                                                                               gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGcc         >  1:5717757/1‑93 (MQ=255)

                                                                                tAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCa        <  2:4625632/93‑1 (MQ=255)

                                                                                tAAATGGCGGCTCTGTCCCATGATTCTGCGTAACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCa        <  1:4164441/93‑1 (MQ=255)

                                                                                 aaaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAt       <  2:2878470/93‑1 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      <  2:3399285/93‑1 (MQ=255)

                                                                                   aTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAttt     <  2:1484138/93‑1 (MQ=255)

                                                                                   aTGGCGGCTCTGGCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAttt     <  2:2063364/93‑1 (MQ=255)

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`