BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,356	T→C	S178S (AGT→AGC)	yecD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,356	0	T	C	76.8%	1790.1 / inf	931	S178S (AGT→AGC)	yecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (104/112); new base C (407/308); total (511/420)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-03

Only 100 of 948 total aligned reads displayed.

CCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCG  >  NC_000913/1949265‑1949448

 cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCAt                                                                                            >  2:5724627/1‑93 (MQ=255)

 cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGAGCCGAGCAACACAATACCAGCAt                                                                                            >  2:3642516/1‑93 (MQ=255)

  cccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATc                                                                                           <  2:5659407/92‑1 (MQ=255)

  aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCAtt                                                                                           >  1:4783624/1‑92 (MQ=255)

    cgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaa                                                                                         >  2:5206750/1‑93 (MQ=255)

      cccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaatc                                                                                       >  2:4319333/1‑91 (MQ=255)

        cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCat                                                                                     <  1:976685/93‑1 (MQ=255)

        cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCat                                                                                     >  2:2162387/1‑93 (MQ=255)

         gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                                    >  1:4641821/1‑93 (MQ=255)

          cAATGCCTGGTAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCatat                                                                                   <  1:2601891/93‑1 (MQ=255)

          cAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCatat                                                                                   >  1:5664432/1‑93 (MQ=255)

           aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATc                                                                                  <  1:4094839/93‑1 (MQ=255)

           aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATc                                                                                  <  1:2084886/93‑1 (MQ=255)

            aTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCt                                                                                 >  1:5279783/1‑93 (MQ=255)

             tGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTa                                                                                <  2:5459744/93‑1 (MQ=255)

             tGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTa                                                                                <  2:2562060/93‑1 (MQ=255)

              gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAc                                                                               <  2:4616202/93‑1 (MQ=255)

               ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAcc                                                                              >  2:2390795/1‑93 (MQ=255)

                cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAccc                                                                             >  2:3255674/1‑93 (MQ=255)

                 tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcg                                                                            >  1:2985032/1‑93 (MQ=255)

                  gggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgc                                                                           >  2:3322607/1‑93 (MQ=255)

                  gggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgc                                                                           >  1:5583942/1‑93 (MQ=255)

                    gAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCgcgc                                                                         >  2:5558287/1‑93 (MQ=255)

                      aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAt                                                                       <  1:5793037/93‑1 (MQ=255)

                      aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAt                                                                       <  2:919030/93‑1 (MQ=255)

                      aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAt                                                                       <  1:3447450/93‑1 (MQ=255)

                      aCTGGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAt                                                                       <  1:3066927/93‑1 (MQ=255)

                       cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                                      >  2:533211/1‑93 (MQ=255)

                       cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                                      >  2:4827982/1‑93 (MQ=255)

                        ttGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATcc                                                                     >  2:5907147/1‑92 (MQ=255)

                        ttGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                     >  2:2714766/1‑93 (MQ=255)

                         tGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGc                                                                    >  1:6089134/1‑93 (MQ=255)

                          ggCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGcc                                                                   <  1:1095881/93‑1 (MQ=255)

                            cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCg                                                                 <  1:428244/93‑1 (MQ=255)

                            cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCg                                                                 >  2:4917829/1‑93 (MQ=255)

                            cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCg                                                                 >  2:349493/1‑93 (MQ=255)

                              ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                               >  2:4029363/1‑93 (MQ=255)

                              ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                               >  1:5886091/1‑93 (MQ=255)

                                aaTCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGtgtg                                                             >  2:5132487/1‑93 (MQ=255)

                                   cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGt                                                          <  1:5319469/93‑1 (MQ=255)

                                   cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGt                                                          >  1:2355149/1‑93 (MQ=255)

                                   cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGt                                                          >  1:3664588/1‑93 (MQ=255)

                                   cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGt                                                          >  1:4400574/1‑93 (MQ=255)

                                      gTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGc                                                       <  1:1378345/93‑1 (MQ=255)

                                          ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                   >  1:200018/1‑93 (MQ=255)

                                          ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                   >  1:1486713/1‑93 (MQ=255)

                                          ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                   <  1:4905759/93‑1 (MQ=255)

                                          ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                   >  2:1249830/1‑93 (MQ=255)

                                           tGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGa                                                  <  2:65076/93‑1 (MQ=255)

                                           tGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGa                                                  <  2:1076077/93‑1 (MQ=255)

                                           tGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGa                                                  <  1:1202017/93‑1 (MQ=255)

                                            gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                                 <  1:5070112/93‑1 (MQ=255)

                                             ccGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAag                                                <  2:3778476/93‑1 (MQ=255)

                                              cGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAaga                                               <  2:2791543/93‑1 (MQ=255)

                                               gAAGACGCCTGTAGGGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAagag                                              <  1:2845356/93‑1 (MQ=255)

                                                aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                             >  1:3990288/1‑93 (MQ=255)

                                                 aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAt                                            <  1:3547650/93‑1 (MQ=255)

                                                 aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAt                                            <  1:4970484/93‑1 (MQ=255)

                                                  gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                           <  1:4509041/93‑1 (MQ=255)

                                                  gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                           <  2:2948630/93‑1 (MQ=255)

                                                  gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                           <  2:2880829/93‑1 (MQ=255)

                                                   aCGCGTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                                          <  2:1317268/93‑1 (MQ=255)

                                                   aCGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                                          <  1:5148563/93‑1 (MQ=255)

                                                    cGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCt                                         >  2:4418394/1‑93 (MQ=255)

                                                    cGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCt                                         <  1:5420928/93‑1 (MQ=255)

                                                        tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAc                                     >  2:780946/1‑93 (MQ=255)

                                                        tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGAGGAAGAGATCCTCAAc                                     >  1:2466212/1‑93 (MQ=255)

                                                        tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                                     >  2:4514268/1‑89 (MQ=255)

                                                          tAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAcgc                                   >  2:424713/1‑93 (MQ=255)

                                                             tGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                                >  1:3218781/1‑93 (MQ=255)

                                                              gccgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTa                               >  1:4139230/1‑93 (MQ=255)

                                                                 gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGa                            <  1:3731880/93‑1 (MQ=255)

                                                                 gccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGa                            >  1:5234214/1‑93 (MQ=255)

                                                                  ccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAt                           >  2:6023301/1‑93 (MQ=255)

                                                                   cAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtt                          <  2:202988/93‑1 (MQ=255)

                                                                   cAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtt                          <  2:1769079/93‑1 (MQ=255)

                                                                     gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTa                        >  2:4697698/1‑93 (MQ=255)

                                                                     gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTa                        >  2:1460871/1‑93 (MQ=255)

                                                                     gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTa                        <  2:1595253/93‑1 (MQ=255)

                                                                      cgcCGAGCAGCACAATAACAGTAACAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTAc                       <  1:1784439/93‑1 (MQ=255)

                                                                      cgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTAc                       >  1:2741122/1‑93 (MQ=255)

                                                                       gctgAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACa                      >  1:5468067/4‑93 (MQ=255)

                                                                       gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACa                      <  2:1402003/93‑1 (MQ=255)

                                                                        ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                     >  1:1917401/1‑93 (MQ=255)

                                                                        ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                     >  1:2540825/1‑93 (MQ=255)

                                                                        ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                     <  2:5856509/93‑1 (MQ=255)

                                                                         cGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATc                    >  2:1148658/1‑93 (MQ=255)

                                                                          gAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCg                   >  1:3358101/1‑93 (MQ=255)

                                                                           agcagcACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCgg                  <  1:775726/93‑1 (MQ=255)

                                                                            gcagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt                 >  1:1607083/1‑93 (MQ=255)

                                                                            gcaacaCAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGt                 >  2:836195/1‑93 (MQ=255)

                                                                             caacacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                <  1:1001413/90‑1 (MQ=255)

                                                                             caacacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                <  1:2204931/90‑1 (MQ=255)

                                                                              aacacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCt               >  1:669887/3‑93 (MQ=255)

                                                                               gcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTa              >  2:3146011/1‑93 (MQ=255)

                                                                                cacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCtgc             <  2:3902463/93‑3 (MQ=255)

                                                                                cacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCtgc             <  2:4198326/93‑3 (MQ=255)

                                                                                  caATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCg           >  1:5986525/1‑93 (MQ=255)

                                                                                    aTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCa         >  2:1893721/1‑93 (MQ=255)

                                                                                        cAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATgg     <  2:1728725/93‑1 (MQ=255)

CCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCG  >  NC_000913/1949265‑1949448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`