Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,9440CA100.0% 755.8 / NA 204Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (97/107);  total (97/107)
*NC_0009133,222,9450AG100.0% 724.2 / NA 204Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (97/107);  total (97/107)
*NC_0009133,222,9460AC100.0% 718.9 / NA 204Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (97/107);  total (97/107)
*NC_0009133,222,9470GA99.5% 727.9 / NA 204A892T (GCC→ACC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (97/106);  minor base C (0/1);  total (97/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 207 total aligned reads displayed.

GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC  >  NC_000913/3222863‑3223026
                                                                                 ||||                                                                               
gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                         >  1:4646550/1‑93 (MQ=255)
 aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                        >  2:2850655/1‑93 (MQ=255)
 aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                        >  1:2605970/1‑93 (MQ=255)
  tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                       <  2:334495/93‑1 (MQ=255)
  tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                       >  1:5334173/1‑93 (MQ=255)
    aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                     >  1:4974640/1‑93 (MQ=255)
     ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt                                                                    >  1:308324/1‑93 (MQ=255)
      gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                   <  2:2076901/93‑1 (MQ=255)
      gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                   <  1:6047166/93‑1 (MQ=255)
      gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                   <  1:5449049/93‑1 (MQ=255)
      gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                   <  2:4014012/93‑1 (MQ=255)
       tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                  >  2:1184744/1‑93 (MQ=255)
       tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                  <  1:2884394/93‑1 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                 <  1:3248001/93‑1 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                 <  1:2081854/93‑1 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                 >  2:2161698/1‑93 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                 >  2:6043272/1‑93 (MQ=255)
         gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                >  1:5798964/1‑93 (MQ=255)
         gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                >  1:3984378/1‑93 (MQ=255)
           gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCga                                                              <  2:1714320/93‑1 (MQ=255)
            tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                             >  2:3046769/1‑93 (MQ=255)
            tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                             >  2:2470344/1‑93 (MQ=255)
              ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                           <  2:272656/93‑1 (MQ=255)
               tACGGTCGTGGACCGGGCGAAACCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                          <  1:4586406/93‑1 (MQ=255)
                   gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATc                                                      >  1:5164859/1‑93 (MQ=255)
                    tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                     >  1:3012627/1‑93 (MQ=255)
                    tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                     <  2:5138608/93‑1 (MQ=255)
                      gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                   <  1:4692295/93‑1 (MQ=255)
                      gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                   >  2:5835709/1‑93 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                  <  2:3692754/93‑1 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                  <  1:5923377/93‑1 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                  >  1:6017354/1‑93 (MQ=255)
                        ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                 <  1:857974/93‑1 (MQ=255)
                        ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                 <  1:110157/93‑1 (MQ=255)
                         gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                <  2:5228577/93‑1 (MQ=255)
                         gACCGGGCGAAAACAACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                <  1:2708866/93‑1 (MQ=255)
                          aCCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTccc                                               >  1:4143366/1‑93 (MQ=255)
                            cGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGc                                             >  1:1998186/1‑93 (MQ=255)
                            cGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGc                                             <  2:4792218/93‑1 (MQ=255)
                            cGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGCCGATGCCATGTTCGTGAACTATACCTTCCCGc                                             >  1:3376659/1‑93 (MQ=255)
                             gggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCa                                            <  1:1026372/93‑1 (MQ=255)
                              ggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAg                                           >  1:44040/1‑93 (MQ=255)
                                 gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                        >  2:2266070/1‑93 (MQ=255)
                                  aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca                                       <  1:1393049/93‑1 (MQ=255)
                                   aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                      <  1:4324861/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                     <  2:3644707/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                     <  2:4167183/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                     >  1:5404744/1‑93 (MQ=255)
                                     aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                    >  2:613898/1‑93 (MQ=255)
                                     aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                    <  1:5880157/93‑1 (MQ=255)
                                      cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg                                   >  1:946770/1‑93 (MQ=255)
                                       tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                  >  1:6115483/1‑93 (MQ=255)
                                       tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                  <  2:4232421/93‑1 (MQ=255)
                                       tACCCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                  <  1:2371839/93‑1 (MQ=255)
                                         cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTaa                                <  2:5899822/93‑1 (MQ=255)
                                         cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTaa                                <  2:1589720/93‑1 (MQ=255)
                                          cccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                               <  1:1936076/92‑1 (MQ=255)
                                           ccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                              >  1:2193138/1‑93 (MQ=255)
                                           ccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                              <  1:4218606/93‑1 (MQ=255)
                                            cGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCg                             <  1:2840564/93‑1 (MQ=255)
                                             gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt                            >  2:3485818/1‑93 (MQ=255)
                                              aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                           <  2:5697665/93‑1 (MQ=255)
                                              aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                           <  1:2487478/93‑1 (MQ=255)
                                                 gccagcGGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCCGAACAACGGTAACCGTCAGc                        >  1:917623/1‑93 (MQ=255)
                                                 gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGc                        >  1:3539201/1‑93 (MQ=255)
                                                   cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                      >  2:4252966/1‑93 (MQ=255)
                                                    agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                     >  2:2410901/1‑93 (MQ=255)
                                                    agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCCTCCCGCAGAACAACGGTAACCGCCAGCATg                     >  1:3629844/1‑93 (MQ=255)
                                                      cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                   <  2:4611739/93‑1 (MQ=255)
                                                       agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                  >  2:2606649/1‑93 (MQ=255)
                                                        ggttAACAACATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                 <  2:2451475/90‑1 (MQ=255)
                                                        ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCCGCGTgcgcg                 >  2:563222/1‑89 (MQ=255)
                                                        ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                 >  2:5392878/1‑93 (MQ=255)
                                                        ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                 >  2:129570/1‑93 (MQ=255)
                                                        ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                 >  2:1738732/1‑93 (MQ=255)
                                                        ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                 >  1:1328246/1‑93 (MQ=255)
                                                         gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                >  1:1545391/1‑93 (MQ=255)
                                                         gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                >  2:3299174/1‑93 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg              >  1:2304366/1‑93 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg              >  1:4543457/1‑93 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg              <  2:5439590/93‑1 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCCTCAGCATGTCCGCTg              >  1:4447527/1‑93 (MQ=255)
                                                            aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg             >  1:373191/1‑93 (MQ=255)
                                                            aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg             >  2:3897139/1‑93 (MQ=255)
                                                            aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg             >  1:4731914/1‑93 (MQ=255)
                                                             aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa            <  2:3545493/93‑1 (MQ=255)
                                                             aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa            <  2:4863870/93‑1 (MQ=255)
                                                             aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa            <  2:1493644/93‑1 (MQ=255)
                                                              catcatCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc           <  1:383291/93‑1 (MQ=255)
                                                              catcatCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc           <  1:1760985/93‑1 (MQ=255)
                                                               atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg          <  1:1105698/93‑1 (MQ=255)
                                                               atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg          <  2:3409387/93‑1 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg         >  2:484992/1‑93 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg         <  2:1014689/93‑1 (MQ=255)
                                                                ccaccGACATCTGGCGCAGCACCGTCGATCGCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg         <  2:399722/89‑1 (MQ=255)
                                                                 catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc        >  2:1968348/1‑93 (MQ=255)
                                                                  ctcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa       <  1:1667941/92‑1 (MQ=255)
                                                                   tcGATATCTGGCGCAGCACCGTCGATGCCATGTACGAGAACTATCCCTTCCCGCAGAACAACGGTAACAGTCAGCATGTCCGCTGGACGGCAc      <  2:5941917/93‑1 (MQ=255)
                                                                    cGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACt     <  1:2951586/93‑1 (MQ=255)
                                                                       tatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAc  <  1:2978303/93‑1 (MQ=255)
                                                                                 ||||                                                                               
GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC  >  NC_000913/3222863‑3223026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑