Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,393,106 Δ1 bp intergenic (‑3443/+857) tldD ← / ← cafA TldD/CafA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,393,1030T.99.6% 1152.0 / ‑3.3 245intergenic (‑3440/+860)tldD/cafATldD/CafA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (131/113);  total (131/114)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA  >  NC_000913/3393011‑3393191
                                                                                            |                                                                                        
cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGt                                                                                          >  1:1619131/1‑93 (MQ=255)
 aGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtt                                                                                         >  2:6041214/1‑93 (MQ=255)
  gCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGttt                                                                                        <  1:6229962/93‑1 (MQ=255)
   cccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttc                                                                                       >  1:4535503/1‑92 (MQ=255)
    ccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttca                                                                                      >  2:1832406/1‑91 (MQ=255)
    ccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttca                                                                                      <  1:3553259/93‑3 (MQ=255)
     cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcag                                                                                     >  1:3289215/1‑90 (MQ=255)
      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                    >  1:6074530/1‑89 (MQ=255)
      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                    >  1:4139651/1‑89 (MQ=255)
      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                    >  1:5184813/1‑89 (MQ=255)
        aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCa                                                                                 >  2:489122/1‑93 (MQ=255)
         ttttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAg                                                                                >  2:5587203/1‑93 (MQ=255)
           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                              <  1:1261877/93‑1 (MQ=255)
           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                              <  2:3344371/93‑1 (MQ=255)
           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTCGCACCGCCCTG‑TTTCAGCCAGAc                                                                              >  1:2567361/1‑93 (MQ=255)
             cACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGt                                                                            >  2:1344695/1‑93 (MQ=255)
             cACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGt                                                                            >  1:2878298/1‑93 (MQ=255)
              aCGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTc                                                                           >  1:4587783/1‑93 (MQ=255)
               cgcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCGCTG‑TTTCAGCCAGACGTCa                                                                          >  1:3615543/1‑93 (MQ=255)
               cgcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTcc                                                                          >  1:6200892/1‑92 (MQ=255)
                ccgTAATAGTCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAc                                                                         <  1:5132217/92‑1 (MQ=255)
                  gTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccg                                                                       <  2:2189474/93‑1 (MQ=255)
                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  1:2395497/93‑1 (MQ=255)
                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      >  1:5003752/1‑93 (MQ=255)
                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  1:1882957/93‑1 (MQ=255)
                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  2:4928386/93‑1 (MQ=255)
                     atatGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgccg                                                                    >  1:547574/1‑93 (MQ=255)
                     atatGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgccg                                                                    >  1:2160553/1‑93 (MQ=255)
                         gcgcGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTa                                                                >  2:1401622/1‑93 (MQ=255)
                            cgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAt                                                             <  1:4063177/93‑1 (MQ=255)
                            cgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAt                                                             <  2:2902951/93‑1 (MQ=255)
                            cgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAt                                                             <  2:4372308/93‑1 (MQ=255)
                             ggTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATc                                                            <  1:3414356/93‑1 (MQ=255)
                              gTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCg                                                           >  2:5834680/1‑93 (MQ=255)
                               tCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGc                                                          >  1:5972037/1‑93 (MQ=255)
                                cAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGcc                                                         <  2:3231924/93‑1 (MQ=255)
                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                       >  2:4819263/1‑93 (MQ=255)
                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                       >  2:6206353/1‑93 (MQ=255)
                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                       <  2:774217/93‑1 (MQ=255)
                                    ttATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTg                                                     <  1:4372330/93‑1 (MQ=255)
                                     tATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGt                                                    <  1:5266530/93‑1 (MQ=255)
                                     tATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGt                                                    >  2:6167955/1‑93 (MQ=255)
                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   >  2:6525350/1‑93 (MQ=255)
                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   >  1:1120523/1‑93 (MQ=255)
                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   >  1:2613092/1‑93 (MQ=255)
                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   <  1:4190447/93‑1 (MQ=255)
                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCTTTTGTCg                                                  <  1:6314380/93‑1 (MQ=255)
                                        ccACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGa                                                 <  2:3416497/93‑1 (MQ=255)
                                        ccACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGa                                                 <  2:2977606/93‑1 (MQ=255)
                                         cACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAt                                                <  1:3362276/93‑1 (MQ=255)
                                           ccGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCg                                              <  1:6492238/93‑1 (MQ=255)
                                           ccGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACAGGCCTG‑TTTCAGCCAGACGTCACTGCCCGTTACATAGCCTTTGTCGATCg                                              >  2:2691764/1‑93 (MQ=255)
                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            >  2:1681470/1‑93 (MQ=255)
                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            <  2:5496774/93‑1 (MQ=255)
                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            >  1:3988971/1‑93 (MQ=255)
                                              aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCa                                           >  2:5235911/1‑93 (MQ=255)
                                               cAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAt                                          <  2:5656629/93‑1 (MQ=255)
                                               cAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAt                                          <  1:5642232/93‑1 (MQ=255)
                                                   gTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAg                                      >  2:3973934/1‑93 (MQ=255)
                                                    tATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGc                                     <  1:221262/93‑1 (MQ=255)
                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                   >  1:205777/1‑93 (MQ=255)
                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                   <  1:5878992/93‑1 (MQ=255)
                                                       gCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCtt                                  <  1:138348/93‑1 (MQ=255)
                                                         gTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCa                                >  1:984812/1‑93 (MQ=255)
                                                          tttGCTTCTCGCCCACCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCCCCTTTGTCGATCGTCATCCAGCCTTCaa                               <  2:4588616/93‑1 (MQ=255)
                                                          tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                               >  1:702355/1‑93 (MQ=255)
                                                          tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                               <  1:400445/93‑1 (MQ=255)
                                                          tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                               <  2:5288281/93‑1 (MQ=255)
                                                           ttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAg                              <  1:282091/93‑1 (MQ=255)
                                                           ttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAg                              <  2:1088465/93‑1 (MQ=255)
                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                            >  1:5786607/1‑93 (MQ=255)
                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                            >  2:1547903/1‑93 (MQ=255)
                                                              cTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGga                           >  1:4934494/1‑93 (MQ=255)
                                                               ttCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgag                          >  2:3367643/1‑93 (MQ=255)
                                                               ttCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgag                          <  2:197785/93‑1 (MQ=255)
                                                                 ctcGCCCAACCAGCTGGGACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGTCGATCGTCATCCAGACTTCAAGGGAGaa                        >  1:3476335/1‑93 (MQ=255)
                                                                  tcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCTAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAc                       <  1:1530931/93‑1 (MQ=255)
                                                                  tcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAc                       <  2:5404576/93‑1 (MQ=255)
                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                    <  2:2452120/93‑1 (MQ=255)
                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                    >  1:885504/1‑93 (MQ=255)
                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                    <  1:2474889/93‑1 (MQ=255)
                                                                       cAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGc                  <  2:3661658/93‑1 (MQ=255)
                                                                        aaCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                 <  2:5135098/93‑1 (MQ=255)
                                                                         aCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCg                >  2:406410/1‑93 (MQ=255)
                                                                           cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGtt              <  2:6613452/93‑1 (MQ=255)
                                                                           cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGtt              >  1:3767943/1‑93 (MQ=255)
                                                                             gCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc            >  1:2956755/1‑93 (MQ=255)
                                                                             gCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc            >  2:3129936/1‑93 (MQ=255)
                                                                             gCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCCTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc            <  2:6287210/93‑1 (MQ=255)
                                                                              cTGGCACCGCCCTG‑TTTGAGCCAGACTTCACCGCCGGTGACATCGCCTTTGTCGATCGTCATCCAGCCTGCAAGGGAGAACTGTGCCGTTTcc           <  2:5990654/93‑1 (MQ=255)
                                                                              cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc           <  1:3100527/93‑1 (MQ=255)
                                                                              cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc           <  2:3863644/93‑1 (MQ=255)
                                                                               tGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa          <  2:1874591/93‑1 (MQ=255)
                                                                               tGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa          >  1:4815094/1‑93 (MQ=255)
                                                                               tGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa          <  2:1227891/93‑1 (MQ=255)
                                                                                ggCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAg         >  2:3760838/1‑93 (MQ=255)
                                                                                ggCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAg         >  2:2071761/1‑93 (MQ=255)
                                                                                 gCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgc        <  2:2540863/93‑1 (MQ=255)
                                                                                     cGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCaa    <  1:102477/93‑1 (MQ=255)
                                                                                       cccTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata  >  1:5433862/1‑93 (MQ=255)
                                                                                            |                                                                                        
CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA  >  NC_000913/3393011‑3393191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑