BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	99.5%	715.4 / ‑3.8	195	coding (162/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (91/103); total (91/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 211 total aligned reads displayed.

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         <  1:1671477/93‑1 (MQ=255)

  cTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑gg                                                                                      >  1:4042364/1‑92 (MQ=255)

    tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcg                                                                                    >  2:3574238/1‑90 (MQ=255)

     cATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcgg                                                                                   >  1:1043009/1‑89 (MQ=255)

                   tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGcc                                                                   <  2:5443203/93‑1 (MQ=255)

                     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCtt                                                                 <  2:6018989/93‑1 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTg                                                                >  1:4844921/1‑93 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTg                                                                >  1:5652505/1‑93 (MQ=255)

                       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGc                                                               >  2:3188507/1‑93 (MQ=255)

                       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGc                                                               >  2:1203665/1‑93 (MQ=255)

                       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGc                                                               <  2:1253862/93‑1 (MQ=255)

                       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGc                                                               >  1:2568519/1‑93 (MQ=255)

                        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGcc                                                              >  2:4103828/1‑93 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCt                                                            <  1:1982322/93‑1 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTg                                                           >  2:2431138/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGa                                                          <  2:2780573/93‑1 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGa                                                          <  2:2721572/93‑1 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:1568719/1‑93 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑CCGGGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:5543126/1‑93 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCa                                                       <  2:1364412/93‑1 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCa                                                       >  1:450480/1‑93 (MQ=255)

                                tGTTGGATTAATGCAAGGACTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:6442314/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATg                                                     <  1:3960345/93‑1 (MQ=255)

                                  ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATgg                                                    <  1:5147565/93‑1 (MQ=255)

                                   tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATggg                                                   >  2:6361687/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:4330833/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:251929/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  1:4289331/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 >  2:5061425/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 >  2:1143453/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  2:3405919/93‑1 (MQ=255)

                                      aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  1:5565124/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               <  1:714461/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGgttt                                               >  1:245279/1‑93 (MQ=255)

                                        tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              <  1:1600414/93‑1 (MQ=255)

                                        tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTg                                              >  1:2643464/1‑93 (MQ=255)

                                         aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                             <  1:4461905/93‑1 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:5086936/1‑93 (MQ=255)

                                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  1:1134039/1‑93 (MQ=255)

                                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           <  1:6057599/93‑1 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCat                                        >  2:5307779/1‑92 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:2051785/93‑1 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  2:4019118/93‑1 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  1:2925756/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:1445923/93‑1 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:6591691/93‑1 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  1:3049343/93‑1 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  1:1741340/93‑1 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  2:3259867/93‑1 (MQ=255)

                                                     gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  1:3533903/1‑93 (MQ=255)

                                                     gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 <  1:6363464/93‑1 (MQ=255)

                                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                >  1:5310863/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:1396757/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  2:313079/1‑93 (MQ=255)

                                                         cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             <  1:3309386/93‑1 (MQ=255)

                                                         cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             >  2:3471556/1‑93 (MQ=255)

                                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  1:1900557/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  2:6639824/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:1158355/1‑93 (MQ=255)

                                                            gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  1:5852474/1‑93 (MQ=255)

                                                             aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         <  2:5715382/93‑1 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCTAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  1:6457958/1‑93 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  1:6007893/1‑93 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:883569/1‑93 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:1384112/1‑93 (MQ=255)

                                                                aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  2:193902/1‑93 (MQ=255)

                                                                 aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  2:3853344/93‑1 (MQ=255)

                                                                  gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  2:1773733/1‑93 (MQ=255)

                                                                  gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  1:1535022/1‑93 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  1:6221403/1‑93 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  1:2490911/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:5388835/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:427361/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:4090412/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:3155322/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:1666485/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  2:1017110/93‑1 (MQ=255)

                                                                       tGGCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  2:1897430/1‑93 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:4214192/93‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  2:2340547/1‑93 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  2:5006057/1‑93 (MQ=255)

                                                                          cTCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            >  1:5225292/1‑93 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  1:2573785/1‑93 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  1:5508448/1‑93 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  1:925054/1‑93 (MQ=255)

                                                                            ccATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          <  1:3127504/93‑1 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  2:821829/1‑93 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  1:5935953/1‑93 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  2:6381927/93‑1 (MQ=255)

                                                                             cATCAAAGCCGGTGTC‑GGAG‑‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  2:5179049/93‑1 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  1:494495/1‑93 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        <  2:1636767/93‑1 (MQ=255)

                                                                                cAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcgc      >  1:628425/1‑93 (MQ=255)

                                                                                cAAAGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcgc      >  2:5903336/1‑93 (MQ=255)

                                                                                 aaaGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca     >  1:1332448/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:2680876/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:5270056/1‑93 (MQ=255)

                                                                                   aGCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCTGTGGCGcaca   >  2:6238831/1‑93 (MQ=255)

                                                                                    gCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:4732363/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGG‑‑CG‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:1744600/93‑1 (MQ=255)

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`