BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,088	+C	coding (2/624 nt)	yhgKJ ←	RNA phosphage cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,088	1	.	C	99.6%	935.3 / NA	261	S133W (TCG→TGG)	yhgKJ	RNA phosphage cyclase
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (140/120); minor base A (0/1); total (140/121)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174

cGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCg                                                                                          <  1:4966334/93‑1 (MQ=255)

 ggCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgc                                                                                         <  2:3184058/93‑2 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgca                                                                                        <  2:811867/93‑3 (MQ=255)

  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgca                                                                                        >  1:6212445/1‑91 (MQ=255)

   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgcag                                                                                       >  2:1194416/1‑90 (MQ=255)

    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgcagg                                                                                      >  2:1717613/1‑89 (MQ=255)

      ggcaaaaaCCGTGGCGCAACAACGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    >  1:227425/4‑93 (MQ=255)

      ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    <  2:1399074/93‑1 (MQ=255)

      ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    <  1:3973591/93‑1 (MQ=255)

      ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    >  1:6258974/1‑93 (MQ=255)

       gTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcgg                                                                                   >  2:3796581/1‑93 (MQ=255)

       gTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATACGCAggcgg                                                                                   >  1:1281717/1‑93 (MQ=255)

        tAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcggc                                                                                  <  2:6101569/93‑1 (MQ=255)

          aaaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGgcgc                                                                                >  2:3841632/1‑93 (MQ=255)

           aaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGcc                                                                               >  2:442533/1‑93 (MQ=255)

           aaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGcc                                                                               <  2:5499797/93‑1 (MQ=255)

             aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGa                                                                             >  1:111990/1‑93 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACgg                                                                          <  2:4814656/93‑1 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACgg                                                                          >  2:3642372/1‑93 (MQ=255)

                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACgg                                                                          <  1:4151671/93‑1 (MQ=255)

                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCCCCCGGCGGATAAAATCCGCAGGCGGCGCCGACggg                                                                         <  1:4611285/93‑1 (MQ=255)

                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACggg                                                                         <  1:3098361/93‑1 (MQ=255)

                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACggg                                                                         >  1:537205/1‑93 (MQ=255)

                 gggCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACggg                                                                         <  2:3955578/92‑1 (MQ=255)

                  ggCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGt                                                                        >  1:6538277/1‑93 (MQ=255)

                     gTAACAGCGTGGGTTGCTGATGAATTCCTATTTTCGCCAGAAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGGCGGGTGAt                                                                     >  2:3502513/1‑93 (MQ=255)

                       aaCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCg                                                                   <  1:5762169/93‑1 (MQ=255)

                       aaCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCg                                                                   >  1:4288848/1‑93 (MQ=255)

                       aaCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTAGCg                                                                   >  1:2025128/1‑93 (MQ=255)

                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCgg                                                                  <  2:342119/93‑1 (MQ=255)

                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  2:5296119/93‑1 (MQ=255)

                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  2:3250052/93‑1 (MQ=255)

                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 >  2:359358/1‑93 (MQ=255)

                           gCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGtgc                                                               >  2:4754036/1‑91 (MQ=255)

                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  1:2915392/93‑4 (MQ=255)

                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  1:5595886/93‑4 (MQ=255)

                              tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑c                                                          <  1:4903116/93‑1 (MQ=255)

                                gTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccg                                                        >  2:618028/1‑91 (MQ=255)

                                gTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCAACCGGCGGATAAAATCCGCAGGAGGCGCCGACGGGTTATCGGt‑gccg‑ccg                                                        >  1:4095223/1‑91 (MQ=255)

                                 tttGCTGATGAATTCCTATTTTCGCGAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgc                                                       <  2:5147453/93‑4 (MQ=255)

                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:2131501/1‑93 (MQ=255)

                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  1:2602583/1‑93 (MQ=255)

                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   <  2:4170311/93‑1 (MQ=255)

                                          gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                               >  2:5652709/1‑93 (MQ=255)

                                           aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              >  1:5914257/1‑93 (MQ=255)

                                           aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              >  1:1474073/1‑93 (MQ=255)

                                           aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              >  1:2760584/1‑93 (MQ=255)

                                            aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                             <  2:5763326/93‑1 (MQ=255)

                                            aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                             <  1:4671257/93‑1 (MQ=255)

                                                cTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGTATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                         <  2:591405/93‑1 (MQ=255)

                                                cTATTCTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                         >  1:4205800/1‑93 (MQ=255)

                                                  aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGaa                                       <  1:4567162/93‑1 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:1905804/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      <  1:1706998/93‑1 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:5403403/1‑93 (MQ=255)

                                                   ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:1206642/1‑93 (MQ=255)

                                                      tCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTc                                   <  1:4036540/93‑1 (MQ=255)

                                                       cGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                  >  1:2450074/1‑93 (MQ=255)

                                                        gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 >  2:1333844/1‑93 (MQ=255)

                                                        gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 >  2:4952955/1‑93 (MQ=255)

                                                        gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 >  1:5131084/1‑93 (MQ=255)

                                                        gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 >  1:3286999/1‑93 (MQ=255)

                                                         ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                >  2:6541795/1‑93 (MQ=255)

                                                         ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                >  2:4073000/1‑93 (MQ=255)

                                                           agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGGCGACGGGTTATCGGTGACGC‑CGCTCACTTCAACACGCGAAGGACCATCg                              >  2:66481/1‑93 (MQ=255)

                                                           agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  1:381166/93‑1 (MQ=255)

                                                           agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  1:3620169/93‑1 (MQ=255)

                                                            gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                             <  1:1006201/93‑1 (MQ=255)

                                                              agcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCa                           >  1:1580292/1‑93 (MQ=255)

                                                              agcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCa                           >  1:4785555/1‑93 (MQ=255)

                                                               gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                          >  2:2797104/1‑93 (MQ=255)

                                                               gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                          >  2:269781/1‑93 (MQ=255)

                                                               gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                          <  1:2099270/93‑1 (MQ=255)

                                                                cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaaa                         >  2:2288482/1‑93 (MQ=255)

                                                                cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaaa                         >  1:5261108/1‑93 (MQ=255)

                                                                  gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACAcc                       >  1:88349/1‑93 (MQ=255)

                                                                  gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       >  2:1542565/1‑93 (MQ=255)

                                                                  gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       <  2:1060842/93‑1 (MQ=255)

                                                                  gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       <  1:3432343/93‑1 (MQ=255)

                                                                  gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       >  1:4365270/1‑93 (MQ=255)

                                                                    tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                     >  2:924141/1‑93 (MQ=255)

                                                                     ccAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                    >  1:3770548/1‑93 (MQ=255)

                                                                      cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaccg                   >  2:6481194/1‑91 (MQ=255)

                                                                      cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   >  1:1153143/1‑93 (MQ=255)

                                                                        gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                 >  2:2585295/1‑93 (MQ=255)

                                                                          aCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg               <  2:5569802/93‑1 (MQ=255)

                                                                           cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              <  1:3066613/93‑1 (MQ=255)

                                                                           cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              >  1:975460/1‑93 (MQ=255)

                                                                           cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              <  1:3164582/93‑1 (MQ=255)

                                                                           cccGGCGGATAAAACCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              <  2:1957745/93‑1 (MQ=255)

                                                                              ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATGGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           <  1:6282827/93‑1 (MQ=255)

                                                                               gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcag          >  1:834440/1‑93 (MQ=255)

                                                                               gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcag          <  2:273705/93‑1 (MQ=255)

                                                                                cggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagc         <  2:5072192/93‑1 (MQ=255)

                                                                                 ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca        <  2:627121/93‑1 (MQ=255)

                                                                                 ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGCCCATCGGCAAACCACAGCGCGGgcagga        >  1:784414/1‑91 (MQ=255)

                                                                                    tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg     >  2:3056935/1‑93 (MQ=255)

                                                                                     aaaaTCCGCAGGCGGCGCCGACGGGATATCGGT‑GCCGCCGCTCACTTCAACACGTCAAGGTGCATCGGCAAACCACAGCGCGGGCAGCACCGt    >  2:5663164/1‑93 (MQ=255)

                                                                                      aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt   <  2:3157576/93‑1 (MQ=255)

                                                                                       aaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt  <  1:5633899/93‑1 (MQ=255)

CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`