BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,835	+G	coding (255/261 nt)	b4507 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,831	1	.	G	99.6%	834.4 / ‑3.5	227	coding (251/261 nt)	b4507	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (123/103); total (123/104)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

Only 100 of 254 total aligned reads displayed.

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTG  >  NC_000913/547739‑547918

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                           <  1:578057/93‑1 (MQ=255)

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                           >  1:649528/1‑93 (MQ=255)

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                           <  2:2101378/93‑1 (MQ=255)

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                           <  1:3550461/93‑1 (MQ=255)

 aTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g                                                                                         <  2:4385664/93‑1 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gg                                                                                      <  2:224043/93‑1 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gg                                                                                      <  2:4017652/93‑1 (MQ=255)

    aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggg                                                                                     >  2:3434721/1‑93 (MQ=255)

    aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggg                                                                                     <  2:6242121/93‑1 (MQ=255)

    aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggg                                                                                     <  2:4733350/93‑1 (MQ=255)

     ccGGACCCATGAAAGGGGCGTGCGTGGTCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggg                                                                                    <  1:2274097/93‑2 (MQ=255)

     ccGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggg                                                                                    <  1:2022192/93‑2 (MQ=255)

      cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggga                                                                                   >  2:6084237/1‑91 (MQ=255)

      cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggga                                                                                   >  2:4278891/1‑91 (MQ=255)

            cATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGaa                                                                              >  1:3943918/1‑93 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                             >  2:3328281/1‑93 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                             <  1:5792834/93‑1 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                             <  1:1051359/93‑1 (MQ=255)

               gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACtt                                                                           <  1:2850515/93‑1 (MQ=255)

                 aaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCa                                                                         <  1:4933699/93‑1 (MQ=255)

                  aGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAt                                                                        <  1:2441648/93‑1 (MQ=255)

                  aGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAt                                                                        <  2:4612787/93‑1 (MQ=255)

                   ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAtt                                                                       >  2:4074271/1‑93 (MQ=255)

                     ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTcc                                                                     >  1:6024303/1‑93 (MQ=255)

                      gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCt                                                                    <  2:2338279/93‑1 (MQ=255)

                        gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTg                                                                  >  2:6613264/1‑93 (MQ=255)

                         tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGt                                                                 >  1:4045271/1‑93 (MQ=255)

                            gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCAc                                                              >  1:2070824/1‑93 (MQ=255)

                             tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCAcc                                                             <  2:5280392/93‑1 (MQ=255)

                               ggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAt                                                           >  2:2529297/1‑93 (MQ=255)

                                gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtg                                                          >  1:3731851/1‑93 (MQ=255)

                                 cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCGCCAtgt                                                         <  2:801243/93‑1 (MQ=255)

                                 cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgt                                                         <  2:6016828/93‑1 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                        >  1:6087250/1‑93 (MQ=255)

                                   cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                                       >  2:3643499/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                     >  1:6606486/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                     >  2:2873275/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                     >  1:162555/1‑93 (MQ=255)

                                      ttCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                    <  1:1529082/92‑1 (MQ=255)

                                      gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                    <  1:3865127/93‑1 (MQ=255)

                                        cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                                                  <  1:3697457/93‑1 (MQ=255)

                                        cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                                                  <  2:2814291/93‑1 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                 <  2:5512232/93‑1 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                 >  2:4847925/1‑93 (MQ=255)

                                          gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                                >  1:1225021/1‑93 (MQ=255)

                                           ttCGAAGGTTGGTCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                               >  1:5223449/1‑93 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                             <  2:3716405/93‑1 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                             >  1:4575101/1‑93 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                             <  2:4837/93‑1 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                            >  2:3868859/1‑93 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                            >  1:4435589/1‑93 (MQ=255)

                                                aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                                          >  1:273000/1‑93 (MQ=255)

                                                aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                                          >  2:1737685/1‑93 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         >  2:5319220/1‑93 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         >  2:6347790/1‑93 (MQ=255)

                                                 ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                         >  2:579537/1‑93 (MQ=255)

                                                   ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                                       <  2:6504754/93‑1 (MQ=255)

                                                    tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTAAACTTCATTCCTTGTCACCATGAGAATGCCGTCGGGCCAATg                                      >  2:3535024/1‑93 (MQ=255)

                                                      ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                    <  1:1753496/93‑1 (MQ=255)

                                                       gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                   <  1:4278605/93‑1 (MQ=255)

                                                       gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                   >  1:1176182/1‑93 (MQ=255)

                                                        cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                                  >  2:5406977/1‑93 (MQ=255)

                                                         gAAAGATGAAGCGCAGGCGCTGGCAGTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg                                 <  2:2018822/93‑1 (MQ=255)

                                                         gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg                                 <  2:5863626/93‑1 (MQ=255)

                                                          aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                >  2:563019/1‑93 (MQ=255)

                                                          aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                >  1:501042/1‑93 (MQ=255)

                                                           aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                               >  1:2529292/1‑93 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                              >  1:5739280/1‑93 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                              <  1:197024/93‑1 (MQ=255)

                                                              aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa                            >  1:3030815/1‑93 (MQ=255)

                                                                 aaGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                         >  2:1467101/1‑93 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                        <  1:4673044/93‑1 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                        <  2:1040464/93‑1 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                        <  1:4875445/93‑1 (MQ=255)

                                                                   gcgcAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                       <  1:3405558/93‑1 (MQ=255)

                                                                   gcgcAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                       >  1:865672/1‑93 (MQ=255)

                                                                       aGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCa                   >  1:3265312/1‑93 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                  >  2:3115466/1‑93 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                  >  1:3134328/1‑93 (MQ=255)

                                                                          cgcTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTa                <  1:5164466/93‑1 (MQ=255)

                                                                          cgcTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTa                <  2:3920768/93‑1 (MQ=255)

                                                                           gcTGGCAATACTGGAGCAGG‑GGGCAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt               >  2:1773818/1‑93 (MQ=255)

                                                                           gcTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt               <  2:4878565/93‑1 (MQ=255)

                                                                           gcTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt               >  1:4924362/1‑93 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc             >  2:4472149/1‑93 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc             <  1:2192614/93‑1 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc             >  1:5388959/1‑93 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGGCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc             >  1:6646407/1‑93 (MQ=255)

                                                                              ggCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGcc            >  1:5529674/1‑93 (MQ=255)

                                                                                cAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGa          <  2:1143122/93‑1 (MQ=255)

                                                                                 aaTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         >  2:3531148/1‑93 (MQ=255)

                                                                                 aaTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         >  1:3453841/1‑93 (MQ=255)

                                                                                 aaTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt         <  1:6516119/93‑1 (MQ=255)

                                                                                 aaTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGGATGCCGag         >  1:1653863/1‑92 (MQ=255)

                                                                                   tACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc       <  2:2572344/93‑1 (MQ=255)

                                                                                   gaCTGCAGCA‑GAGGGAAGTGATGTTCATTCCTTGTCACAATGTAAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc       <  2:417854/92‑1 (MQ=255)

                                                                                    aCTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCt      <  1:4343/93‑1 (MQ=255)

                                                                                     cTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtg     <  2:1246430/93‑1 (MQ=255)

                                                                                      tGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtgg    >  1:1008257/1‑93 (MQ=255)

                                                                                       ggAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggt   >  1:563220/1‑93 (MQ=255)

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTG  >  NC_000913/547739‑547918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`