BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,411	0	A	C	49.7%	143.8 / inf	499	N197H (AAC→CAC)	yecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (145/106); new base C (138/110); total (283/216)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 518 total aligned reads displayed.

CCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACT  >  NC_000913/1949319‑1949503

ccTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCa                                                                                              >  1:1623553/1‑93 (MQ=255)

  tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAc                                                                                            <  2:630101/93‑1 (MQ=255)

  tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAc                                                                                            >  1:4556729/1‑93 (MQ=255)

      gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGt                                                                                        <  2:3524008/93‑1 (MQ=255)

       tGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGtt                                                                                       <  2:6434828/93‑1 (MQ=255)

       tGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                                                                                       <  1:5858766/93‑1 (MQ=255)

        gccgccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTa                                                                                      <  2:5182011/93‑1 (MQ=255)

        gccgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAGCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTa                                                                                      <  2:3237054/93‑1 (MQ=255)

          cgccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATg                                                                                    >  1:4338174/1‑93 (MQ=255)

             cAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                                                                 <  1:2881793/93‑1 (MQ=255)

             cAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                                                                 >  1:5043383/1‑93 (MQ=255)

              aGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAttt                                                                                <  1:6569936/93‑1 (MQ=255)

              aGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAttt                                                                                <  1:890607/93‑1 (MQ=255)

               gcgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTa                                                                               >  1:2662919/1‑93 (MQ=255)

                cgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTAc                                                                              >  1:1486424/1‑93 (MQ=255)

                 gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACa                                                                             <  1:3210481/93‑1 (MQ=255)

                  ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                                                                            <  2:2973762/93‑1 (MQ=255)

                   cGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATc                                                                           >  1:1413567/1‑93 (MQ=255)

                    gAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCg                                                                          >  1:1101113/1‑93 (MQ=255)

                    gAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAACAGATCCTCAACGCGTTATGATTTACATCg                                                                          >  2:113293/1‑93 (MQ=255)

                       cagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTc                                                                       <  1:261182/93‑1 (MQ=255)

                       caacacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                                                                       <  1:5141731/90‑1 (MQ=255)

                        agcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCt                                                                      >  2:4344844/1‑93 (MQ=255)

                        agcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCt                                                                      >  1:5452110/1‑93 (MQ=255)

                        agcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCt                                                                      >  1:4691842/1‑93 (MQ=255)

                         acacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCtg                                                                     >  2:2800341/2‑92 (MQ=255)

                          cacaATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAc                                                                    <  2:67748/93‑1 (MQ=255)

                           acaATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAcc                                                                   <  1:3390729/93‑1 (MQ=255)

                             aaTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGc                                                                 <  1:5732122/93‑1 (MQ=255)

                             aaTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGc                                                                 <  1:2193280/93‑1 (MQ=255)

                               tAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCaa                                                               >  1:3709854/1‑93 (MQ=255)

                               tAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCaa                                                               >  1:3521660/1‑93 (MQ=255)

                                aaCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAAt                                                              >  1:2252331/1‑93 (MQ=255)

                                aaCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAAt                                                              >  1:6324543/1‑93 (MQ=255)

                                aaCAGCATTAAGCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAAt                                                              <  1:3839086/93‑1 (MQ=255)

                                 aCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATg                                                             <  1:6280460/93‑1 (MQ=255)

                                  cAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATgg                                                            <  2:225186/93‑1 (MQ=255)

                                   aGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGt                                                           >  1:2048593/1‑93 (MQ=255)

                                      aTCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGc                                                        <  2:6257968/93‑1 (MQ=255)

                                      aTCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGc                                                        >  2:2058603/1‑93 (MQ=255)

                                        cAAGCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCAt                                                      <  2:889593/93‑1 (MQ=255)

                                           tcATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCt                                                   >  2:5114957/2‑93 (MQ=255)

                                              tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTaaa                                                <  1:3040565/93‑1 (MQ=255)

                                              tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTaaa                                                >  1:5060317/1‑93 (MQ=255)

                                              tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTaaa                                                >  1:1025271/1‑93 (MQ=255)

                                              tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTaaa                                                >  1:1999935/1‑93 (MQ=255)

                                                tctcCCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATg                                              <  2:2378142/93‑1 (MQ=255)

                                                 cTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATgg                                             <  1:725921/93‑1 (MQ=255)

                                                 cTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATgg                                             <  1:2393221/93‑1 (MQ=255)

                                                 cTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATgg                                             >  2:4568760/1‑93 (MQ=255)

                                                  tACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATggg                                            >  2:3726514/1‑93 (MQ=255)

                                                    cccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTg                                          <  2:1185065/93‑1 (MQ=255)

                                                    cccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTg                                          <  1:3912548/93‑1 (MQ=255)

                                                    cccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTg                                          <  2:4418712/93‑1 (MQ=255)

                                                     ccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGc                                         >  1:5605060/1‑93 (MQ=255)

                                                      ggcgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCg                                        >  1:6385181/2‑93 (MQ=255)

                                                      cgcgCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCg                                        >  2:3676488/1‑93 (MQ=255)

                                                      cgcgCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCg                                        >  1:4036635/1‑93 (MQ=255)

                                                      cgcgCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCg                                        <  1:4156482/93‑1 (MQ=255)

                                                       gcgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCgg                                       >  2:4522422/1‑93 (MQ=255)

                                                        cgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGt                                      >  1:2423439/1‑93 (MQ=255)

                                                        cgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGt                                      >  2:6121037/1‑93 (MQ=255)

                                                         gcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGtt                                     >  1:1202365/1‑93 (MQ=255)

                                                          cATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTg                                    <  2:5610039/93‑1 (MQ=255)

                                                           aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                   >  2:1785921/1‑93 (MQ=255)

                                                           aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                   >  1:6131413/1‑93 (MQ=255)

                                                           aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                   >  2:4200397/1‑93 (MQ=255)

                                                           aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                   >  2:5835521/1‑93 (MQ=255)

                                                           aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                   <  1:4425969/93‑1 (MQ=255)

                                                             cGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTgggt                                 >  2:2677603/1‑92 (MQ=255)

                                                             cGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTgggg                                 <  2:1683880/93‑1 (MQ=255)

                                                              gCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGa                                >  2:3894608/1‑93 (MQ=255)

                                                               cccGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGAt                               <  1:1747446/93‑1 (MQ=255)

                                                                 cGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCa                             >  1:5952533/1‑93 (MQ=255)

                                                                  gtgtGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCAc                            >  1:4884636/1‑93 (MQ=255)

                                                                   tgtgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCAcc                           >  1:330635/1‑93 (MQ=255)

                                                                    gtgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCa                          <  2:1048608/93‑1 (MQ=255)

                                                                        gTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCt                      >  1:526218/1‑93 (MQ=255)

                                                                          aGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTATCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTg                    >  2:4549609/1‑93 (MQ=255)

                                                                           gCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGa                   >  1:4224615/1‑93 (MQ=255)

                                                                           gCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGa                   <  1:2780182/93‑1 (MQ=255)

                                                                           gCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGa                   >  2:1333174/1‑93 (MQ=255)

                                                                             gTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAag                 <  2:546587/93‑1 (MQ=255)

                                                                              tGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAaga                >  1:816000/1‑93 (MQ=255)

                                                                              gggAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAaga                <  2:2575703/92‑1 (MQ=255)

                                                                               ggAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAagag               >  1:2698011/1‑93 (MQ=255)

                                                                                 aaGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTa             >  2:4768452/1‑93 (MQ=255)

                                                                                 aaGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTa             <  2:5361383/93‑1 (MQ=255)

                                                                                 aaGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTa             >  2:3096997/1‑93 (MQ=255)

                                                                                  agagATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTAt            <  2:4654679/93‑1 (MQ=255)

                                                                                  agagATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTAt            <  1:1322355/93‑1 (MQ=255)

                                                                                   gagaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATg           >  2:1881909/1‑93 (MQ=255)

                                                                                    agaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGc          <  2:499971/93‑1 (MQ=255)

                                                                                     gaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGcc         <  2:5986426/93‑1 (MQ=255)

                                                                                      aTCCTTCACGCGTTATGATTTACATCGGTCTGCCGAAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGccc        <  2:752961/93‑1 (MQ=255)

                                                                                       tCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCg       >  2:3460355/1‑93 (MQ=255)

                                                                                         cTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGcc     <  2:314893/93‑1 (MQ=255)

                                                                                          tCAACGCGTTATTATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCa    <  2:250920/93‑1 (MQ=255)

                                                                                           cAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCat   >  2:4055528/1‑92 (MQ=255)

                                                                                            aaCGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACt  <  2:445862/93‑1 (MQ=255)

CCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACT  >  NC_000913/1949319‑1949503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`