BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,526	A→G	V75A (GTG→GCG)	ynhG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,526	0	A	G	88.5%	599.7 / 58.5	217	V75A (GTG→GCG)	ynhG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (17/8); new base G (95/97); total (112/105)
Fisher's exact test for biased strand distribution p-value = 9.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 220 total aligned reads displayed.

AGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTC  >  NC_000913/1756436‑1756614

aGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGcc                                                                                        >  1:6245543/1‑93 (MQ=255)

 gCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCg                                                                                       >  1:6412614/1‑93 (MQ=255)

 gCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCg                                                                                       >  2:1931011/1‑93 (MQ=255)

 gCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGGTTTGGCGCCg                                                                                       >  1:5097678/1‑93 (MQ=255)

  ctctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCgg                                                                                      <  1:1234230/93‑1 (MQ=255)

  ctctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCgg                                                                                      >  1:6019060/1‑93 (MQ=255)

   tctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCggg                                                                                     <  1:5321641/93‑1 (MQ=255)

    ctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCgggg                                                                                    <  2:6094568/93‑1 (MQ=255)

    ctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCgggg                                                                                    <  1:3687644/93‑1 (MQ=255)

    ctGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCgggg                                                                                    <  1:3351576/93‑1 (MQ=255)

     tGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGc                                                                                   >  2:2470082/1‑93 (MQ=255)

      gCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCTGGGGCg                                                                                  >  2:2824998/1‑93 (MQ=255)

        aaGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGCAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGAt                                                                                >  2:5215084/1‑93 (MQ=255)

        aaGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGAt                                                                                >  2:5401666/1‑93 (MQ=255)

         aGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATa                                                                               >  1:3252232/1‑93 (MQ=255)

         aGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATa                                                                               >  1:4032726/1‑93 (MQ=255)

         aGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATa                                                                               <  2:5109006/93‑1 (MQ=255)

          ggTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAg                                                                              <  2:1220580/93‑1 (MQ=255)

           gTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAgt                                                                             <  2:2335229/93‑1 (MQ=255)

            ttAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGTGCCGGGGCGATAgtg                                                                            >  2:3376032/1‑93 (MQ=255)

            ttAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAgtg                                                                            >  2:1446995/1‑93 (MQ=255)

             tAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAgtgt                                                                           <  2:4857099/93‑1 (MQ=255)

              aaCGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGtt                                                                          <  2:541747/93‑1 (MQ=255)

               aCGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTa                                                                         <  2:320494/93‑1 (MQ=255)

                 gATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTAtt                                                                       >  1:3551556/1‑93 (MQ=255)

                 gATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTAtt                                                                       <  2:5479748/93‑1 (MQ=255)

                    aaTCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGc                                                                    >  1:4662311/1‑93 (MQ=255)

                      tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCtt                                                                  >  2:1400158/1‑93 (MQ=255)

                      tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCtt                                                                  >  2:3122183/1‑93 (MQ=255)

                         ccTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCaa                                                               <  1:1332214/93‑1 (MQ=255)

                         ccTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCaa                                                               >  1:4921123/1‑93 (MQ=255)

                          cTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAg                                                              <  1:2114788/93‑1 (MQ=255)

                          cTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAg                                                              >  1:3309850/1‑93 (MQ=255)

                             aCGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGAt                                                           >  1:3429359/1‑93 (MQ=255)

                             aCGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGAt                                                           <  1:898932/93‑1 (MQ=255)

                             aCGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGAt                                                           >  2:270420/1‑93 (MQ=255)

                                cgGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCaa                                                        >  2:4084894/1‑93 (MQ=255)

                                cgGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCaa                                                        >  1:3912928/1‑93 (MQ=255)

                                  gTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACa                                                      >  1:5599542/1‑93 (MQ=255)

                                   tGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACAt                                                     >  1:650346/1‑93 (MQ=255)

                                   tGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACAt                                                     <  1:1280128/93‑1 (MQ=255)

                                      aTCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGc                                                  <  1:5481076/93‑1 (MQ=255)

                                      aTCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGc                                                  <  1:1528772/93‑1 (MQ=255)

                                       tCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTgtc                                                 >  1:5002901/1‑91 (MQ=255)

                                       tCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGcc                                                 <  1:687952/93‑1 (MQ=255)

                                       tCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGcc                                                 <  2:1533796/93‑1 (MQ=255)

                                        cAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCg                                                >  2:3703549/1‑93 (MQ=255)

                                         aGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGc                                               <  1:6179400/93‑1 (MQ=255)

                                           gTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAg                                             >  1:5820783/1‑93 (MQ=255)

                                           gTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCACAg                                             >  2:1723338/1‑93 (MQ=255)

                                           gTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAg                                             <  2:828208/93‑1 (MQ=255)

                                              ataaCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTAt                                          <  2:4685824/93‑1 (MQ=255)

                                               taaCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATc                                         >  1:3860235/1‑93 (MQ=255)

                                                aaCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCa                                        <  2:2125705/93‑1 (MQ=255)

                                                  cAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCaaa                                      >  1:4415486/1‑93 (MQ=255)

                                                  cAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCaaa                                      >  2:6266319/1‑93 (MQ=255)

                                                   aGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCaaaa                                     <  1:1860191/93‑1 (MQ=255)

                                                    gTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCaaaag                                    >  2:1010247/1‑92 (MQ=255)

                                                     ttGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACg                                   <  1:1015654/93‑1 (MQ=255)

                                                      tgtgAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGt                                  <  1:5687797/93‑1 (MQ=255)

                                                       gtgAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTc                                 >  1:2333999/1‑93 (MQ=255)

                                                        tgAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCg                                <  2:5713697/93‑1 (MQ=255)

                                                         gAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCgg                               <  2:2918569/93‑1 (MQ=255)

                                                         gAAGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCgg                               >  1:2965375/1‑93 (MQ=255)

                                                           aGGAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGc                             >  1:2623099/1‑93 (MQ=255)

                                                            ggAATAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCa                            <  1:3640617/93‑1 (MQ=255)

                                                            ggAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCa                            >  2:1060588/1‑93 (MQ=255)

                                                              aaTAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAAt                          <  1:2937767/93‑1 (MQ=255)

                                                              aaTAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAAt                          >  2:1655875/1‑93 (MQ=255)

                                                              aaTAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAAt                          <  2:1997371/93‑1 (MQ=255)

                                                               aTAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATa                         <  2:5792920/93‑1 (MQ=255)

                                                               aTAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATa                         <  2:1842737/93‑1 (MQ=255)

                                                               aTAGTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATa                         >  2:1238597/1‑93 (MQ=255)

                                                               aTAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATa                         <  2:6631239/93‑1 (MQ=255)

                                                                  gTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGcc                      >  2:2518490/1‑93 (MQ=255)

                                                                  gTTATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGcc                      <  2:6447037/93‑1 (MQ=255)

                                                                   ttATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCt                     >  1:3145522/1‑93 (MQ=255)

                                                                   ttATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCt                     >  1:4855986/1‑93 (MQ=255)

                                                                    tATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTg                    <  1:3058893/93‑1 (MQ=255)

                                                                    tATCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTg                    >  1:2213781/1‑93 (MQ=255)

                                                                     aTCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGa                   >  1:4596802/1‑93 (MQ=255)

                                                                     aTCGTCGTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGa                   <  1:4933856/93‑1 (MQ=255)

                                                                      tcgtcgTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGaa                  <  2:6568619/93‑1 (MQ=255)

                                                                      tcgtcgTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGaa                  >  2:1463710/1‑93 (MQ=255)

                                                                      tcgtcgTACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGaa                  <  1:1549101/93‑1 (MQ=255)

                                                                       cgtcgtACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAg                 <  2:961493/93‑1 (MQ=255)

                                                                       cgtcgtACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAg                 >  1:1379562/1‑93 (MQ=255)

                                                                       cgtcgtACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAg                 >  1:3456379/1‑93 (MQ=255)

                                                                       cgtcgtACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAg                 <  1:5394198/93‑1 (MQ=255)

                                                                        gtcgtACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAGGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAgg                >  2:4310878/1‑93 (MQ=255)

                                                                            tACCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGtttt            >  2:4597355/1‑93 (MQ=255)

                                                                             aCCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGttttt           <  1:1648729/93‑1 (MQ=255)

                                                                             aCCAGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGttttt           >  1:1423303/1‑93 (MQ=255)

                                                                               cAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTAt         >  1:2456459/1‑93 (MQ=255)

                                                                               cAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTAt         >  2:2786918/1‑93 (MQ=255)

                                                                                aGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATc        <  1:2027556/93‑1 (MQ=255)

                                                                                aGGTTTTGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATc        >  1:4622983/1‑93 (MQ=255)

                                                                                   ttttGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATcacc     >  2:2079162/1‑93 (MQ=255)

                                                                                    tttGGCGCCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCct    <  2:1973753/93‑1 (MQ=255)

                                                                                      tGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCcttc  <  2:4471707/93‑1 (MQ=255)

AGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTC  >  NC_000913/1756436‑1756614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`