BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,774,277	A→G	M223V (ATG→GTG)	ydiF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,774,277	0	A	G	82.4%	523.9 / 103.2	222	M223V (ATG→GTG)	ydiF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (15/24); new base G (86/97); total (101/121)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01

Only 100 of 244 total aligned reads displayed.

ACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTC  >  NC_000913/1774185‑1774366

aCAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGtga                                                                                           >  2:3785282/1‑93 (MQ=255)

  aGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTgtggtg                                                                                         >  2:3197455/1‑93 (MQ=255)

    tGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTggtgat                                                                                       <  2:4122996/93‑1 (MQ=255)

     gAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGgtgatg                                                                                      >  2:1837632/1‑93 (MQ=255)

       aGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCa                                                                                    >  2:5762959/1‑93 (MQ=255)

        ggCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAg                                                                                   >  1:3763628/1‑93 (MQ=255)

         gCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAgg                                                                                  <  2:1251690/93‑1 (MQ=255)

         gCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAgg                                                                                  >  1:5621769/1‑93 (MQ=255)

          cTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGt                                                                                 <  1:1395042/93‑1 (MQ=255)

          cTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTAATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGt                                                                                 <  2:44630/93‑1 (MQ=255)

           tACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTg                                                                                >  1:4608310/1‑93 (MQ=255)

           tACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTg                                                                                <  1:3686693/93‑1 (MQ=255)

              gCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAg                                                                             >  1:63308/1‑93 (MQ=255)

              gCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAg                                                                             >  1:4272001/1‑93 (MQ=255)

                cACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGaa                                                                           <  1:3501601/93‑1 (MQ=255)

                cACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGaa                                                                           >  1:6013819/1‑93 (MQ=255)

                 aCTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGaaa                                                                          >  1:4106858/1‑93 (MQ=255)

                  cTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCa                                                                              >  2:4169096/1‑88 (MQ=255)

                  cTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGaaaa                                                                         <  2:5118766/93‑1 (MQ=255)

                  cTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGaaaa                                                                         >  2:5482762/1‑93 (MQ=255)

                   ttttGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGCATTGTGATGATGCAGGGGCAGAAAAt                                                                        >  1:883062/1‑93 (MQ=255)

                      tGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGt                                                                     >  1:1126622/1‑93 (MQ=255)

                       gAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGtt                                                                    <  1:2310522/93‑1 (MQ=255)

                        aaGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTa                                                                   <  2:6190233/93‑1 (MQ=255)

                         aGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTaa                                                                  >  1:2391702/1‑93 (MQ=255)

                          gATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGGGATGCAGGCGCAGAAAATGGTTaaa                                                                 >  2:1353796/1‑92 (MQ=255)

                              aGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAg                                                             >  2:4809413/1‑93 (MQ=255)

                                gTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGcc                                                           <  2:2816778/93‑1 (MQ=255)

                                gTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGcc                                                           <  1:4663125/93‑1 (MQ=255)

                                  gATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCAc                                                         <  2:5890087/93‑1 (MQ=255)

                                  gATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCAc                                                         <  2:4939210/93‑1 (MQ=255)

                                    tGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGc                                                       <  2:2878980/93‑1 (MQ=255)

                                    tGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGc                                                       <  2:2260417/93‑1 (MQ=255)

                                       aTCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGc                                                    <  1:6638334/93‑1 (MQ=255)

                                        tctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCa                                                   >  1:5024874/1‑93 (MQ=255)

                                         ctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCAt                                                  <  2:3743729/93‑1 (MQ=255)

                                            gACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCt                                               <  2:3511151/93‑1 (MQ=255)

                                             aCGCATTGATTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTa                                              >  1:6473592/1‑93 (MQ=255)

                                               gCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTaaa                                            >  2:6618456/1‑93 (MQ=255)

                                               gCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTaaa                                            >  1:3283175/1‑93 (MQ=255)

                                                cATTTGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAAt                                           <  2:4885848/93‑1 (MQ=255)

                                                cATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAAt                                           >  1:282956/1‑93 (MQ=255)

                                                 aTTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                          >  2:2722193/1‑93 (MQ=255)

                                                 aTTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                          <  1:3009423/93‑1 (MQ=255)

                                                 aTTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                          <  1:6073165/93‑1 (MQ=255)

                                                  ttGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCt                                         >  2:4845009/1‑93 (MQ=255)

                                                  ttGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCt                                         <  1:6368979/93‑1 (MQ=255)

                                                  ttGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCt                                         <  1:281274/93‑1 (MQ=255)

                                                    ggTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGt                                       <  2:4004374/93‑1 (MQ=255)

                                                    ggTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGt                                       <  1:3044098/93‑1 (MQ=255)

                                                    ggTTATTGCCAGGGCGGTGCA‑AATAAAGGCGGTATTGTTGGGATGGAGGGGAAAAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTc                                      <  1:3611102/93‑1 (MQ=255)

                                                     gTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTc                                      <  2:5441897/93‑1 (MQ=255)

                                                     gTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTc                                      <  2:2000600/93‑1 (MQ=255)

                                                      ttATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTcc                                     <  2:701872/93‑1 (MQ=255)

                                                      gtATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTcc                                     <  2:5544158/92‑1 (MQ=255)

                                                        aTTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGt                                   <  2:3692019/93‑1 (MQ=255)

                                                         ttGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTa                                  >  1:2551526/1‑93 (MQ=255)

                                                           gCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTAtt                                >  1:2717841/1‑93 (MQ=255)

                                                            cccAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTc                               >  1:4552740/1‑93 (MQ=255)

                                                            cccAGGCGGTGCACAATAACGGCGGGATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTc                               >  2:2116558/1‑93 (MQ=255)

                                                              cAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCg                             >  1:5962791/1‑93 (MQ=255)

                                                              cAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCg                             <  1:1227900/93‑1 (MQ=255)

                                                              cAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCg                             <  2:4263608/93‑1 (MQ=255)

                                                              cAGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCg                             >  2:6334221/1‑93 (MQ=255)

                                                               aGGCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCgg                            >  1:1078840/1‑93 (MQ=255)

                                                                ggCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCggg                           >  1:1181255/1‑93 (MQ=255)

                                                                ggCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCggg                           <  1:1338090/93‑1 (MQ=255)

                                                                ggCGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGCTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCggg                           >  1:4736831/1‑93 (MQ=255)

                                                                 gCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGt                          <  2:564796/93‑1 (MQ=255)

                                                                  cGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGtt                         >  2:4683644/1‑93 (MQ=255)

                                                                  cGGTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGtt                         <  1:4374680/93‑1 (MQ=255)

                                                                   ggTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTa                        <  1:5108898/93‑1 (MQ=255)

                                                                    gTGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTAt                       <  2:2042704/93‑1 (MQ=255)

                                                                     tGCACAATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATc                      >  2:352588/1‑93 (MQ=255)

                                                                       cacaATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTTCGTATTCCGGGTTATCtg                    >  2:911488/1‑93 (MQ=255)

                                                                        acaATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtgg                   <  2:3894624/93‑1 (MQ=255)

                                                                         caATAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggt                  <  1:2184706/93‑1 (MQ=255)

                                                                         caATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggt                  >  2:3266076/1‑93 (MQ=255)

                                                                           aTAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggtgg                >  1:5399625/1‑93 (MQ=255)

                                                                           aTAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggtgg                >  2:2596457/1‑93 (MQ=255)

                                                                            tAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGa               >  1:1621190/1‑93 (MQ=255)

                                                                            tAACGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGa               <  1:2126820/93‑1 (MQ=255)

                                                                              aCGGCGGTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGata             >  2:849818/1‑93 (MQ=255)

                                                                              aCGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGata             >  2:1666010/1‑93 (MQ=255)

                                                                               cggcggTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGatat            <  1:2247906/93‑1 (MQ=255)

                                                                                   ggTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATtgtg        <  1:1925382/93‑1 (MQ=255)

                                                                                   ggTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATtgtg        <  2:2511297/93‑1 (MQ=255)

                                                                                    gTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg       <  2:5171657/93‑1 (MQ=255)

                                                                                    gTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg       >  1:5444320/1‑93 (MQ=255)

                                                                                    gTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg       >  1:6165997/1‑93 (MQ=255)

                                                                                    gTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg       <  2:2142116/93‑1 (MQ=255)

                                                                                    gTATTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg       >  1:1460389/1‑93 (MQ=255)

                                                                                      aTTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtggtg     >  2:1002533/1‑93 (MQ=255)

                                                                                      aTTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtggtg     <  2:5055915/93‑1 (MQ=255)

                                                                                      aTTGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGATATCTGGTGGATATTgtggtg     >  1:4233303/1‑93 (MQ=255)

                                                                                       ttGTGGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGtggtgg    >  1:5724133/1‑93 (MQ=255)

                                                                                       ttGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGtggtgg    >  1:1829869/1‑93 (MQ=255)

                                                                                        tgtgGTGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTggtggt   <  1:5921278/93‑1 (MQ=255)

                                                                                         gtggtgATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTc  <  2:3243473/93‑1 (MQ=255)

                                                                                         gtggtgATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTc  <  1:3821219/93‑1 (MQ=255)

ACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTC  >  NC_000913/1774185‑1774366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`