BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,414	T→C	Y495H (TAT→CAT)	yeaG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,414	0	T	C	86.7%	619.0 / 77.7	225	Y495H (TAT→CAT)	yeaG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (17/13); new base C (92/103); total (109/116)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 232 total aligned reads displayed.

CAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACC  >  NC_000913/1866322‑1866503

cagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCt                                                                                           >  2:1252508/1‑93 (MQ=255)

  gAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCAt                                                                                         >  1:3424804/1‑93 (MQ=255)

  aaaCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCAt                                                                                         <  2:2135328/92‑1 (MQ=255)

    acacTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGCCGGCCTACCTTGAATCCCATTc                                                                                       <  1:3743083/90‑1 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       >  2:2732092/1‑93 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       <  2:5922157/93‑1 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       <  2:5199837/93‑1 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   >  2:5377564/1‑93 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   <  2:6560817/93‑1 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGATTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   >  1:5067194/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  >  1:6219236/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  <  1:818280/93‑1 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  >  1:1916679/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTGATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  <  1:1356345/93‑1 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAata                                                                                 >  2:2881637/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 >  1:5991482/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 >  1:2579744/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 <  2:3817151/93‑1 (MQ=255)

           cTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAatat                                                                                >  2:647438/1‑93 (MQ=255)

            tGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCCAATATg                                                                               >  1:3862718/1‑93 (MQ=255)

              gAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATggg                                                                             >  1:4900597/1‑93 (MQ=255)

                  tCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaa                                                                         >  2:1130866/1‑93 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        >  2:4060470/1‑93 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        <  1:4222086/93‑1 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        <  2:5987098/93‑1 (MQ=255)

                     tGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACa                                                                      <  2:3216138/93‑1 (MQ=255)

                     tGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACa                                                                      >  1:2060516/1‑93 (MQ=255)

                      gAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCACAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAt                                                                     <  2:3048921/93‑1 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  1:1059901/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  2:4195862/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  2:5069685/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    <  1:1004466/93‑1 (MQ=255)

                        aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAttt                                                                   <  2:5464861/93‑1 (MQ=255)

                        aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAttt                                                                   >  1:4796711/1‑93 (MQ=255)

                           gTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCg                                                                <  1:4100209/93‑1 (MQ=255)

                             tATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAc                                                              <  2:1918780/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  2:1809883/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:6375996/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:2666205/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:3633176/93‑1 (MQ=255)

                               tCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCg                                                            <  2:814840/93‑1 (MQ=255)

                               tCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCg                                                            >  1:6482636/1‑93 (MQ=255)

                                cTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGt                                                           <  2:5758372/93‑1 (MQ=255)

                                 tGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGtt                                                          <  1:1285665/93‑1 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         >  2:805978/1‑93 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         <  1:1832482/93‑1 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         >  1:3555329/1‑93 (MQ=255)

                                   aTCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTAt                                                        >  2:1980392/1‑93 (MQ=255)

                                    tCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATg                                                       <  2:6417142/93‑1 (MQ=255)

                                       cGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTa                                                    <  1:4431259/93‑1 (MQ=255)

                                        gAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                                                   >  1:5090559/1‑93 (MQ=255)

                                        gAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTAACTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                                                   >  2:4670505/1‑93 (MQ=255)

                                         aaaTATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGGAAAACATTTTCGACCGTTATGTTAcc                                                  >  1:5356964/1‑93 (MQ=255)

                                          aaTATGCCGAGTTTATCGTCAAAGAGATCCAGACGGCCTACCTTGAATCTTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCt                                                 <  1:579622/93‑1 (MQ=255)

                                           atatGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTa                                                <  2:744404/93‑1 (MQ=255)

                                             atGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcg                                              <  1:570014/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:3816656/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:566120/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:288913/93‑1 (MQ=255)

                                                 cGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGa                                          >  1:3380866/1‑93 (MQ=255)

                                                 cGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGa                                          >  2:3562337/1‑93 (MQ=255)

                                                   aGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGAtt                                        >  1:523564/1‑93 (MQ=255)

                                                      ttATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt                                     >  1:1654795/1‑93 (MQ=255)

                                                      ttAACGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt                                     <  2:6359773/93‑1 (MQ=255)

                                                         tCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGa                                  <  2:5615279/93‑1 (MQ=255)

                                                           ggCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGAtt                                <  2:2438383/93‑1 (MQ=255)

                                                           ggCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGAtt                                <  2:3494322/93‑1 (MQ=255)

                                                             cAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtca                              <  1:2083587/93‑1 (MQ=255)

                                                              aaaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcag                             <  1:4000061/93‑1 (MQ=255)

                                                              aaaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcag                             >  2:3078524/1‑93 (MQ=255)

                                                               aaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagg                            <  1:6091231/93‑1 (MQ=255)

                                                               aaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagg                            >  2:449597/1‑93 (MQ=255)

                                                                agagATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagga                           <  2:3360023/93‑1 (MQ=255)

                                                                 gagaTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggat                          >  1:3648147/1‑93 (MQ=255)

                                                                 gagaTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggat                          >  1:3076559/1‑93 (MQ=255)

                                                                    aTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcag                       >  2:4036579/1‑93 (MQ=255)

                                                                     tCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagg                      >  2:1575540/1‑93 (MQ=255)

                                                                      ccAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagga                     >  1:4495618/1‑93 (MQ=255)

                                                                      ccAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagga                     >  1:4707010/1‑93 (MQ=255)

                                                                         gACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTa                  <  2:6206782/93‑1 (MQ=255)

                                                                          aCGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTAt                 <  2:2756252/93‑1 (MQ=255)

                                                                           cGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATc                >  2:4260865/1‑93 (MQ=255)

                                                                             gCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATcgc              >  1:1238987/1‑93 (MQ=255)

                                                                             gCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATcgc              >  1:2295062/1‑93 (MQ=255)

                                                                               cTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGa            <  1:2128348/93‑1 (MQ=255)

                                                                               cTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGa            >  1:3945013/1‑93 (MQ=255)

                                                                                tACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAt           >  1:5851595/1‑93 (MQ=255)

                                                                                tACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAt           <  2:1687807/93‑1 (MQ=255)

                                                                                  ccTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         >  1:305537/1‑93 (MQ=255)

                                                                                  ccTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         <  1:4861011/93‑1 (MQ=255)

                                                                                  ccTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         >  2:4508927/1‑93 (MQ=255)

                                                                                   cTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        >  1:6609812/1‑93 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        <  1:5487403/93‑1 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        >  2:6634189/1‑93 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        <  1:962354/93‑1 (MQ=255)

                                                                                     tGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGa      <  2:3124191/93‑1 (MQ=255)

                                                                                      gAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGAt     >  1:4312004/1‑93 (MQ=255)

                                                                                       aaTCCCATTCCGAATATGGGCAAAACGTTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCCCGATCCGGATa    <  1:240184/93‑1 (MQ=255)

                                                                                         tCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATAcc  <  2:4838731/93‑1 (MQ=255)

                                                                                         tCCCATACCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATAcc  <  1:2534992/93‑1 (MQ=255)

CAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACC  >  NC_000913/1866322‑1866503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`