BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,990	G→A	T194T (ACG→ACA)	yeaI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,990	0	G	A	86.8%	574.4 / 54.0	190	T194T (ACG→ACA)	yeaI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (12/13); new base A (93/72); total (105/85)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 194 total aligned reads displayed.

TTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTA  >  NC_000913/1868900‑1869079

 ttCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGa                                                                                        >  1:5257555/1‑93 (MQ=255)

 ttCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGa                                                                                        <  1:5081851/93‑1 (MQ=255)

   ccGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAAt                                                                                      <  2:6197350/93‑1 (MQ=255)

    cGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATc                                                                                     <  1:6002000/93‑1 (MQ=255)

        aaCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGt                                                                                 >  2:5969980/1‑93 (MQ=255)

        aaCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGt                                                                                 >  2:2625200/1‑93 (MQ=255)

        aaCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGt                                                                                 <  1:6042199/93‑1 (MQ=255)

         aCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGtt                                                                                >  1:3506119/1‑93 (MQ=255)

         aCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGtt                                                                                <  1:5649130/93‑1 (MQ=255)

         aCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGtt                                                                                >  2:4561774/1‑93 (MQ=255)

           tCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGc                                                                              <  2:5099376/93‑1 (MQ=255)

            cAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCt                                                                             >  2:6380686/1‑93 (MQ=255)

                tttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAACAAAACAGGAATCCTGTTGCTGgcg                                                                         >  1:3374500/1‑93 (MQ=255)

                tttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgcg                                                                         >  2:2468130/1‑93 (MQ=255)

                 ttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgcgc                                                                        >  1:4592947/1‑93 (MQ=255)

                  tATTTGTTTACCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCt                                                                       <  1:2777766/93‑1 (MQ=255)

                    tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGa                                                                     >  1:1320802/1‑93 (MQ=255)

                    tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGa                                                                     <  2:842347/93‑1 (MQ=255)

                    tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGa                                                                     <  1:901819/93‑1 (MQ=255)

                    tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGa                                                                     <  1:2680466/93‑1 (MQ=255)

                       gTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATcc                                                                  >  2:2805572/1‑93 (MQ=255)

                       gTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATcc                                                                  >  1:4356788/1‑93 (MQ=255)

                        tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATccc                                                                 >  1:3548618/1‑93 (MQ=255)

                        tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATccc                                                                 <  2:1469887/93‑1 (MQ=255)

                        tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATccc                                                                 <  2:1741455/93‑1 (MQ=255)

                          tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCtt                                                               >  1:4758411/1‑93 (MQ=255)

                           aaCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCttt                                                              <  2:6596387/93‑1 (MQ=255)

                            aCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttt                                                             >  1:755644/1‑93 (MQ=255)

                            aCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttt                                                             >  1:3887639/1‑93 (MQ=255)

                              cTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCtttttt                                                           >  2:1076285/1‑93 (MQ=255)

                              cTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCtttttt                                                           <  2:3473723/93‑1 (MQ=255)

                              cTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCtttttt                                                           >  2:4433917/1‑93 (MQ=255)

                               tCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTa                                                          <  1:1052937/93‑1 (MQ=255)

                               tCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTa                                                          >  1:5598857/1‑93 (MQ=255)

                                cGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAg                                                         >  2:5153335/1‑93 (MQ=255)

                                cGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAg                                                         >  2:4900480/1‑93 (MQ=255)

                                 gCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGt                                                        <  1:1826721/93‑1 (MQ=255)

                                  cTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGtt                                                       <  2:1702772/93‑1 (MQ=255)

                                   tGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGttt                                                      <  1:6213890/93‑1 (MQ=255)

                                    ggCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGtttt                                                     >  1:1614498/1‑93 (MQ=255)

                                      cgcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTc                                                   <  1:4119505/93‑1 (MQ=255)

                                      cgcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTc                                                   <  2:810450/93‑1 (MQ=255)

                                       gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcc                                                  <  2:1724907/93‑1 (MQ=255)

                                       gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcc                                                  >  1:3711585/1‑93 (MQ=255)

                                       gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcc                                                  <  2:6285510/93‑1 (MQ=255)

                                       gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcc                                                  >  2:6070678/1‑93 (MQ=255)

                                        ctctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTccc                                                 >  2:6100464/1‑93 (MQ=255)

                                        ctctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTccc                                                 >  2:5095088/1‑93 (MQ=255)

                                         tctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcccc                                                >  2:2328962/1‑93 (MQ=255)

                                          ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCt                                               >  2:5620345/1‑93 (MQ=255)

                                          ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCt                                               <  1:638443/93‑1 (MQ=255)

                                          ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCt                                               >  2:3970169/1‑93 (MQ=255)

                                           ttttGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCtt                                              >  1:143979/1‑93 (MQ=255)

                                            tttGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTc                                             <  2:1002110/93‑1 (MQ=255)

                                                  aTGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcac                                       >  1:774379/1‑93 (MQ=255)

                                                    ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacac                                     >  1:610001/1‑93 (MQ=255)

                                                    ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacac                                     >  2:3733110/1‑93 (MQ=255)

                                                    ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacac                                     <  1:4913223/93‑1 (MQ=255)

                                                     gAAAAGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacaca                                    >  1:1563312/1‑93 (MQ=255)

                                                      aaaaGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACaa                                   <  1:991740/93‑1 (MQ=255)

                                                        aaGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATc                                 >  2:1608474/1‑93 (MQ=255)

                                                        aaGACAACATCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATc                                 >  2:4291980/1‑93 (MQ=255)

                                                           acaacaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGa                              <  1:2331371/93‑1 (MQ=255)

                                                           acaacaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGa                              >  1:3178897/1‑93 (MQ=255)

                                                             aacaTCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                            >  1:2446601/1‑93 (MQ=255)

                                                             aacaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                            >  2:1392660/1‑93 (MQ=255)

                                                             aacaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                            >  2:3910407/1‑93 (MQ=255)

                                                              acaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCa                           <  2:2715930/93‑1 (MQ=255)

                                                              acaTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCa                           <  1:2536116/93‑1 (MQ=255)

                                                               caTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAg                          <  2:421313/93‑1 (MQ=255)

                                                                aTCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGt                         >  2:5897781/1‑93 (MQ=255)

                                                                 tCCTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGtt                        >  1:5807005/1‑93 (MQ=255)

                                                                   cTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtat                      <  1:3517825/93‑1 (MQ=255)

                                                                   cTTGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtat                      >  2:3628260/1‑93 (MQ=255)

                                                                    ttGACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtata                     >  2:5070908/1‑93 (MQ=255)

                                                                      gACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAAc                   >  1:2170731/1‑93 (MQ=255)

                                                                      gACAACAATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAAc                   <  2:800770/93‑1 (MQ=255)

                                                                       acaacaATAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACg                  >  1:1108470/1‑93 (MQ=255)

                                                                        caacaaTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGc                 <  2:3335481/93‑1 (MQ=255)

                                                                         aacaaTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCt                >  1:4169047/1‑93 (MQ=255)

                                                                           caaTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa              >  1:3190121/1‑93 (MQ=255)

                                                                           caaTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa              <  2:3577590/93‑1 (MQ=255)

                                                                           caaTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa              <  1:2389749/93‑1 (MQ=255)

                                                                             aTAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACt            >  2:3123990/1‑93 (MQ=255)

                                                                             aTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACt            >  2:1217489/1‑93 (MQ=255)

                                                                             aTAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACt            <  1:5038162/93‑1 (MQ=255)

                                                                              tAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTa           >  2:190616/1‑93 (MQ=255)

                                                                              tAAGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTa           <  1:4491196/93‑1 (MQ=255)

                                                                               aaGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAt          >  2:751828/1‑93 (MQ=255)

                                                                                aGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt         >  2:4941131/1‑93 (MQ=255)

                                                                                aGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt         >  1:1981507/1‑93 (MQ=255)

                                                                                aGAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt         >  1:4735348/1‑93 (MQ=255)

                                                                                 gAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAttt        >  2:2819671/1‑92 (MQ=255)

                                                                                 gAAAAAAACAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTc        >  2:2119614/1‑93 (MQ=255)

                                                                                   aaaaaaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCtt      <  1:2030356/93‑1 (MQ=255)

                                                                                    aaaaaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCttt     <  1:6377688/93‑1 (MQ=255)

                                                                                     aaaaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg    >  1:6636310/1‑93 (MQ=255)

                                                                                     aaaaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg    >  2:6626529/1‑93 (MQ=255)

                                                                                       aaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa  <  1:3204146/93‑1 (MQ=255)

                                                                                       aaCAGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa  <  1:3311536/93‑1 (MQ=255)

TTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTA  >  NC_000913/1868900‑1869079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`