BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,206	T→C	S108P (TCC→CCC)	yeaJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,206	0	T	C	86.2%	520.7 / 73.2	203	S108P (TCC→CCC)	yeaJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (17/11); new base C (92/83); total (109/94)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

Only 100 of 214 total aligned reads displayed.

GTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGA  >  NC_000913/1870116‑1870297

gtgCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGActcc                                                                                           <  2:1606435/93‑1 (MQ=255)

gtgCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGAcccc                                                                                           <  1:3598691/93‑1 (MQ=255)

 tgCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGAcccct                                                                                          <  1:1210698/93‑1 (MQ=255)

 tgCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGAcccct                                                                                          >  1:6620066/1‑93 (MQ=255)

  gCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGAcccctc                                                                                         >  1:1281066/1‑93 (MQ=255)

    tGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCcctcct                                                                                       <  1:6335868/93‑1 (MQ=255)

      aCATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCcttt                                                                                     <  1:821395/93‑1 (MQ=255)

        aTCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCctttct                                                                                   >  2:435317/1‑93 (MQ=255)

         tCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCctttctt                                                                                  <  2:3123403/93‑1 (MQ=255)

         tCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCctttctt                                                                                  <  2:1773761/93‑1 (MQ=255)

          cttccttcCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCctttcttt                                                                                 >  2:167124/1‑93 (MQ=255)

           ttccttccTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTa                                                                                >  2:2591981/1‑93 (MQ=255)

            tccttcctTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTAt                                                                               <  2:3451838/93‑1 (MQ=255)

            tccttcctTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTAt                                                                               <  1:369418/93‑1 (MQ=255)

             ccttccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATg                                                                              <  2:5607910/93‑1 (MQ=255)

              cttccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGa                                                                             >  1:1471592/1‑93 (MQ=255)

               ttccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGAt                                                                            <  2:2544364/93‑1 (MQ=255)

                tccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATa                                                                           >  1:222431/1‑93 (MQ=255)

                tccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATa                                                                           >  1:2482669/1‑93 (MQ=255)

                   ttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGt                                                                        >  2:2327961/1‑93 (MQ=255)

                   ttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGt                                                                        >  1:2158333/1‑93 (MQ=255)

                    tATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTa                                                                       <  1:2336958/93‑1 (MQ=255)

                     aTTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTat                                                                      <  1:1768366/93‑1 (MQ=255)

                     aTTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTat                                                                      >  1:4078906/1‑93 (MQ=255)

                       ttttGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatca                                                                    <  2:224154/93‑1 (MQ=255)

                          tGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaa                                                                 <  1:2950661/93‑1 (MQ=255)

                           gAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaat                                                                >  2:2749682/1‑93 (MQ=255)

                            aaCTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatc                                                               >  1:4257290/1‑93 (MQ=255)

                            aaCTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatc                                                               >  1:334173/1‑93 (MQ=255)

                            aaCTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatc                                                               <  2:4012135/93‑1 (MQ=255)

                              cTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatcag                                                             >  2:2108505/1‑93 (MQ=255)

                              cTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatcag                                                             >  1:916632/1‑93 (MQ=255)

                               ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                            >  2:6499045/1‑93 (MQ=255)

                               ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                            <  2:4780787/93‑1 (MQ=255)

                               ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                            <  1:5130210/93‑1 (MQ=255)

                                tGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCagag                                                           <  2:617262/93‑1 (MQ=255)

                                    gCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTAtt                                                       <  2:1830195/93‑1 (MQ=255)

                                    gCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTAtt                                                       >  2:408010/1‑93 (MQ=255)

                                     ccAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTg                                                      >  1:3417457/1‑93 (MQ=255)

                                     ccAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTg                                                      >  1:6100989/1‑93 (MQ=255)

                                      cAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGc                                                     <  2:2844503/93‑1 (MQ=255)

                                       aGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGcc                                                    >  2:4742110/1‑93 (MQ=255)

                                        gCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCcg                                                   >  1:1680455/1‑93 (MQ=255)

                                         cGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCcgc                                                  <  2:4384619/93‑1 (MQ=255)

                                            cAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCa                                               >  2:2975903/1‑93 (MQ=255)

                                              gagGAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAtg                                             >  2:3836622/1‑93 (MQ=255)

                                              gagCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAtg                                             >  2:3678544/1‑93 (MQ=255)

                                                gCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAtgtg                                           <  1:1894772/93‑1 (MQ=255)

                                                 cAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGa                                          >  2:4045093/1‑93 (MQ=255)

                                                 cAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGa                                          <  2:5395167/93‑1 (MQ=255)

                                                  gaTGAGTGCCTATCTGCGCTATATCGTGCAGAACGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCCCATGTGAt                                         <  2:3282829/92‑1 (MQ=255)

                                                  aaTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGAt                                         >  1:1409946/1‑93 (MQ=255)

                                                  aaTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGAt                                         >  2:3539080/1‑93 (MQ=255)

                                                  aaTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGAt                                         >  1:3005465/1‑93 (MQ=255)

                                                  aaTGAGTGACTATCTGCGCAATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGAt                                         >  1:6442457/1‑93 (MQ=255)

                                                   aTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATg                                        <  1:2204368/93‑1 (MQ=255)

                                                   aTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATg                                        >  1:5847093/1‑93 (MQ=255)

                                                   aTGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATg                                        >  2:5796480/1‑93 (MQ=255)

                                                    tGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgc                                       <  2:1565294/93‑1 (MQ=255)

                                                    tGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgc                                       <  1:3965235/93‑1 (MQ=255)

                                                    tGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgc                                       <  1:3636394/93‑1 (MQ=255)

                                                      aGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgcgc                                     <  2:52901/93‑1 (MQ=255)

                                                         gCCTATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGct                                  >  2:6078470/1‑93 (MQ=255)

                                                            tATCTGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCtctc                               >  2:1454683/1‑93 (MQ=255)

                                                                tGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctg                           >  2:956244/1‑93 (MQ=255)

                                                                tGCGCTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctg                           <  2:1731303/93‑1 (MQ=255)

                                                                 gcgcTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctgc                          <  1:3119876/93‑1 (MQ=255)

                                                                 gcgcTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctgc                          <  1:6033649/93‑1 (MQ=255)

                                                                  cgcTATATCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctgct                         <  1:4419884/93‑1 (MQ=255)

                                                                   gcTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGctgctg                        <  1:3764794/93‑1 (MQ=255)

                                                                     tataTCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAg                      <  2:1557607/93‑1 (MQ=255)

                                                                     tataTCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAg                      >  2:6376770/1‑93 (MQ=255)

                                                                     tataTCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAg                      >  1:1638904/1‑93 (MQ=255)

                                                                     tataTCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAg                      >  2:4568944/1‑93 (MQ=255)

                                                                      atatCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGc                     <  2:2372350/93‑1 (MQ=255)

                                                                       tatCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGAATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCa                    >  1:718985/1‑93 (MQ=255)

                                                                        atCGTGCGGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAg                   >  1:2045425/1‑93 (MQ=255)

                                                                        atCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAg                   >  2:1220494/1‑93 (MQ=255)

                                                                         tCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGt                  >  1:6527724/1‑93 (MQ=255)

                                                                         tCGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGt                  <  1:2737656/93‑1 (MQ=255)

                                                                          cGTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTc                 <  1:3985985/93‑1 (MQ=255)

                                                                           gTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCg                >  2:1935749/1‑93 (MQ=255)

                                                                           gTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGCGCAGTCg                <  1:5372024/93‑1 (MQ=255)

                                                                           gTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCg                <  2:4925667/93‑1 (MQ=255)

                                                                           gTGCAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCg                <  1:1919399/93‑1 (MQ=255)

                                                                            tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCgg               <  2:1142069/93‑1 (MQ=255)

                                                                             gCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGa              <  2:697019/93‑1 (MQ=255)

                                                                              cAGAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGaa             <  2:5509771/93‑1 (MQ=255)

                                                                                gAAAGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAgt           >  1:4890251/1‑93 (MQ=255)

                                                                                 aaaGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAgtg          >  2:572052/1‑93 (MQ=255)

                                                                                  aaGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAgtgt         >  1:4343523/1‑93 (MQ=255)

                                                                                   aGCAGTCTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTc        >  2:2357281/1‑93 (MQ=255)

                                                                                   aGCAGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTc        <  1:1049899/93‑1 (MQ=255)

                                                                                      aGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGcc     <  1:6226913/93‑1 (MQ=255)

                                                                                      aGACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGcc     >  1:758622/1‑93 (MQ=255)

                                                                                       gACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCa    >  2:5182291/1‑93 (MQ=255)

                                                                                       gACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCa    >  1:4491896/1‑93 (MQ=255)

                                                                                       gACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCa    >  2:944776/1‑93 (MQ=255)

                                                                                       gACCCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGACa    >  2:2048720/1‑93 (MQ=255)

                                                                                         ctcctcCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGa  >  2:5436595/1‑93 (MQ=255)

GTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGA  >  NC_000913/1870116‑1870297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`