BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,205	A→G	V195V (GTA→GTG)	accA →	acetyl‑CoA carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,205	0	A	G	85.2%	627.2 / 92.1	250	V195V (GTA→GTG)	accA	acetyl‑CoA carboxylase
Reads supporting (aligned to +/- strand): ref base A (17/20); new base G (88/125); total (105/145)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 258 total aligned reads displayed.

GGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTC  >  NC_000913/209113‑209297

   gCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGtt                                                                                           <  2:3981491/93‑1 (MQ=255)

   gCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGtt                                                                                           <  1:3336010/93‑1 (MQ=255)

     ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTg                                                                                         >  2:1211018/1‑93 (MQ=255)

     ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTg                                                                                         >  1:350724/1‑93 (MQ=255)

      tATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGt                                                                                        <  1:1818525/93‑1 (MQ=255)

         ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACg                                                                                     <  2:1314576/93‑1 (MQ=255)

         ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACg                                                                                     >  1:3292132/1‑93 (MQ=255)

         ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACg                                                                                     >  2:4296432/1‑93 (MQ=255)

           tGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGt                                                                                   <  1:5066537/93‑1 (MQ=255)

            ggCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGtt                                                                                  <  2:5327866/93‑1 (MQ=255)

             gCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTa                                                                                 <  1:6364248/93‑1 (MQ=255)

               gTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATc                                                                               <  1:3681508/93‑1 (MQ=255)

               gTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATc                                                                               <  2:3938192/93‑1 (MQ=255)

                     gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGaa                                                                         >  1:2225914/1‑93 (MQ=255)

                     gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGaa                                                                         <  1:3099669/93‑1 (MQ=255)

                       agaagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAgg                                                                       >  1:2006934/1‑93 (MQ=255)

                       agaagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAgg                                                                       <  2:4492641/93‑1 (MQ=255)

                        gaagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAggt                                                                      >  2:5134163/1‑93 (MQ=255)

                         aagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAggtg                                                                     <  1:3917980/93‑1 (MQ=255)

                           gagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAggtggt                                                                   >  1:3418111/1‑93 (MQ=255)

                           gagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAggtggt                                                                   <  1:2100820/93‑1 (MQ=255)

                           gagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAggtggt                                                                   <  1:3788124/93‑1 (MQ=255)

                            agCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGtt                                                                  <  1:2277476/93‑1 (MQ=255)

                             gCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTc                                                                 <  2:2474469/93‑1 (MQ=255)

                              ggTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAAGGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCt                                                                <  1:3457555/92‑1 (MQ=255)

                              cgtcgtCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCt                                                                <  2:2575984/93‑1 (MQ=255)

                                   tCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCgg                                                           <  1:5998841/93‑1 (MQ=255)

                                   tCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCgg                                                           <  2:250819/93‑1 (MQ=255)

                                      gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGGGAAGGTGGTTCTGGCGGTgc                                                        >  2:6324214/1‑93 (MQ=255)

                                        gtGAAGCCATTGCACGCAACCTGCGTGAAACGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcgc                                                      <  2:4364469/92‑1 (MQ=255)

                                        cTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTGCCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcgc                                                      <  2:5866314/93‑1 (MQ=255)

                                         tGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCt                                                     <  1:4433554/93‑1 (MQ=255)

                                         tGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCt                                                     <  1:5510182/93‑1 (MQ=255)

                                         tGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCt                                                     <  2:1335547/93‑1 (MQ=255)

                                          gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTg                                                    <  1:4978997/93‑1 (MQ=255)

                                          gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTg                                                    >  1:1466302/1‑93 (MQ=255)

                                          gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTg                                                    >  2:3576749/1‑93 (MQ=255)

                                           aaGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTgg                                                   <  2:3451450/93‑1 (MQ=255)

                                            aGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGc                                                  >  2:6593213/1‑93 (MQ=255)

                                             gCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCg                                                 >  2:816940/1‑93 (MQ=255)

                                               cATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAt                                               >  2:2079193/1‑93 (MQ=255)

                                                aTTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                              <  1:4134411/93‑1 (MQ=255)

                                                aTTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                              >  1:4764192/1‑93 (MQ=255)

                                                 ttGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTg                                             <  1:4081122/93‑1 (MQ=255)

                                                 ttGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTg                                             <  1:2918369/93‑1 (MQ=255)

                                                 ttGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTg                                             <  2:442365/93‑1 (MQ=255)

                                                  tGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                            >  1:1088527/1‑93 (MQ=255)

                                                  tGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                            <  2:3674973/93‑1 (MQ=255)

                                                  tGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                            <  2:4060355/93‑1 (MQ=255)

                                                   gCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGc                                           <  1:3185406/93‑1 (MQ=255)

                                                   gCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGc                                           >  1:2950910/1‑93 (MQ=255)

                                                    cACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                          >  1:1276764/1‑93 (MQ=255)

                                                     aCGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGt                                         <  1:1187263/93‑1 (MQ=255)

                                                     aCGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGt                                         >  1:1529665/1‑93 (MQ=255)

                                                      cGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTg                                        >  2:3092644/1‑93 (MQ=255)

                                                      cGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTg                                        <  1:5226217/93‑1 (MQ=255)

                                                       gCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTgg                                       >  2:4274697/1‑93 (MQ=255)

                                                       gCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTgg                                       >  1:703167/1‑93 (MQ=255)

                                                        cAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTggg                                      <  1:785671/93‑1 (MQ=255)

                                                        cAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTggg                                      <  2:6137770/93‑1 (MQ=255)

                                                         aaCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTgggg                                     >  2:2406253/1‑92 (MQ=255)

                                                          aCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                    >  2:4967549/1‑93 (MQ=255)

                                                           ccTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGa                                   <  2:1611311/93‑1 (MQ=255)

                                                           ccTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGa                                   >  1:5301847/1‑93 (MQ=255)

                                                            cTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAt                                  >  1:160085/1‑93 (MQ=255)

                                                            cTGCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAt                                  <  2:6223971/93‑1 (MQ=255)

                                                             tGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATa                                 <  1:5696941/93‑1 (MQ=255)

                                                              gCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaa                                >  1:454294/1‑93 (MQ=255)

                                                              gCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaa                                >  2:2244699/1‑93 (MQ=255)

                                                              gCGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaa                                >  2:5454563/1‑93 (MQ=255)

                                                               cGTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaaa                               <  2:6004883/93‑1 (MQ=255)

                                                                gTGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAg                              >  2:6090835/1‑93 (MQ=255)

                                                                 tGAAATGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                             >  1:1331254/1‑93 (MQ=255)

                                                                 tGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGCAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAgg                             >  2:1706003/1‑92 (MQ=255)

                                                                    aaTGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGAGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGaa                          <  2:311253/93‑1 (MQ=255)

                                                                     aTGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAat                         >  1:2788165/1‑93 (MQ=255)

                                                                      tGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAata                        <  2:6405184/93‑1 (MQ=255)

                                                                      tGTCTCGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAata                        <  2:1421617/93‑1 (MQ=255)

                                                                          tcGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgct                    >  2:4873673/1‑93 (MQ=255)

                                                                          tcGCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgct                    <  1:3028776/93‑1 (MQ=255)

                                                                            gCCTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctgc                  >  2:3203782/1‑93 (MQ=255)

                                                                             ccTCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCa                 >  2:2896126/1‑93 (MQ=255)

                                                                               tCGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAAt               <  1:6099551/93‑1 (MQ=255)

                                                                               tCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAAt               <  1:2621269/93‑1 (MQ=255)

                                                                               ccGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAAt               <  2:1101700/92‑1 (MQ=255)

                                                                                cGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATa              <  1:3223936/93‑1 (MQ=255)

                                                                                cGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATa              >  1:2135643/1‑93 (MQ=255)

                                                                                cGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATa              <  2:5885265/93‑1 (MQ=255)

                                                                                cGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATa              >  1:6222973/1‑93 (MQ=255)

                                                                                cGGCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATa              >  2:1984214/1‑93 (MQ=255)

                                                                                  gCGTGCCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACa            <  1:5991733/93‑1 (MQ=255)

                                                                                  gCGTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACa            <  1:4682820/93‑1 (MQ=255)

                                                                                    gTACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGc          >  1:3979348/1‑93 (MQ=255)

                                                                                     tACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCa         <  1:5136163/93‑1 (MQ=255)

                                                                                     tACCGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCa         <  2:3392002/93‑1 (MQ=255)

                                                                                     tACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGcc         >  2:174641/1‑92 (MQ=255)

                                                                                     tACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCa         >  2:1161782/1‑93 (MQ=255)

                                                                                        cGGTGGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCt      <  1:4654507/93‑1 (MQ=255)

                                                                                         ggtggtTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTa     <  1:4879290/93‑1 (MQ=255)

                                                                                         ggTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTa     >  2:2671555/1‑93 (MQ=255)

GGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTC  >  NC_000913/209113‑209297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`