BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,417	T→C	H337R (CAC→CGC)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,417	0	T	C	85.0%	596.4 / 85.9	233	H337R (CAC→CGC)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base T (16/19); new base C (106/92); total (122/111)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 238 total aligned reads displayed.

GTAGTCCCAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCA  >  NC_000913/2115325‑2115509

gTAGTCCCAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGt                                                                                              >  1:2036761/1‑93 (MQ=255)

    tCCCAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAg                                                                                          <  2:5014048/93‑1 (MQ=255)

    tCCCAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAg                                                                                          <  1:4814630/93‑1 (MQ=255)

     cgcAGCTGCTGCATAATCCCGGGGGATTGATGTTCGTAGTTGATGGAAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAgg                                                                                         <  2:4624756/91‑1 (MQ=255)

     cccAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAgg                                                                                         >  2:6554136/1‑93 (MQ=255)

     cccAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAgg                                                                                         <  1:1329986/93‑1 (MQ=255)

     cccAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAgg                                                                                         >  2:4300678/1‑93 (MQ=255)

     cccAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAgg                                                                                         >  2:278412/1‑93 (MQ=255)

     cccAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAAGTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAgg                                                                                         <  1:6136414/93‑1 (MQ=255)

         gctgctGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGcgcg                                                                                     <  2:229367/93‑1 (MQ=255)

          ctgctgCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGt                                                                                    >  2:4864991/1‑93 (MQ=255)

           tgctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTa                                                                                   >  1:3750974/1‑93 (MQ=255)

           tgctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTa                                                                                   >  2:1389294/1‑93 (MQ=255)

           tgctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTa                                                                                   <  2:508903/93‑1 (MQ=255)

            gctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTAc                                                                                  <  1:1522568/93‑1 (MQ=255)

             ctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTAcc                                                                                 <  2:4976607/93‑1 (MQ=255)

             ctgcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTAcc                                                                                 <  2:2169313/93‑1 (MQ=255)

               gcATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGa                                                                               <  2:2269196/93‑1 (MQ=255)

                cATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGAc                                                                              <  1:2085819/93‑1 (MQ=255)

                  tAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACgg                                                                            >  1:2882273/1‑93 (MQ=255)

                    aTCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTg                                                                          <  2:6494567/93‑1 (MQ=255)

                    aTCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGAGCAGGCGCGTACCGACggcg                                                                          >  1:356390/1‑91 (MQ=255)

                     tCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGa                                                                         <  2:4931738/93‑1 (MQ=255)

                      cccGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAg                                                                        <  1:5376566/93‑1 (MQ=255)

                      cccGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAg                                                                        <  2:3198155/93‑1 (MQ=255)

                       ccGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGt                                                                       <  1:4868619/93‑1 (MQ=255)

                         ggcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTc                                                                     >  2:3297093/1‑93 (MQ=255)

                         ggcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTc                                                                     >  1:1068952/1‑93 (MQ=255)

                          gcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTca                                                                    >  2:1572206/1‑93 (MQ=255)

                          gcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTca                                                                    <  1:6045717/93‑1 (MQ=255)

                          gcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTca                                                                    >  2:2870032/1‑93 (MQ=255)

                          gcggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTca                                                                    >  1:3309035/1‑93 (MQ=255)

                           cggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTcac                                                                   >  2:5985177/1‑93 (MQ=255)

                           cggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTcac                                                                   >  1:2193612/1‑93 (MQ=255)

                            ggATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGGGAGTTcaca                                                                  >  2:2370950/1‑93 (MQ=255)

                             gATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAg                                                                 >  2:5163884/1‑93 (MQ=255)

                             gATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAg                                                                 <  2:4379637/93‑1 (MQ=255)

                              aTTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAgg                                                                >  2:4236806/1‑93 (MQ=255)

                              aTTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAgg                                                                >  2:6395820/1‑93 (MQ=255)

                               tttATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGc                                                               >  1:2437716/1‑93 (MQ=255)

                                ttATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGcc                                                              >  2:1116274/1‑93 (MQ=255)

                                  aTGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGcccc                                                            >  1:958638/1‑93 (MQ=255)

                                  aTGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGcccc                                                            <  1:4948180/93‑1 (MQ=255)

                                   tGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGccccc                                                           <  1:2755788/93‑1 (MQ=255)

                                       cGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGaa                                                       <  1:5489940/93‑1 (MQ=255)

                                       cGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGaa                                                       <  2:102802/93‑1 (MQ=255)

                                         tAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAAtt                                                     >  2:5447469/1‑93 (MQ=255)

                                         tAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAAtt                                                     >  1:2480198/1‑93 (MQ=255)

                                           gTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAAtttt                                                   >  2:388133/1‑93 (MQ=255)

                                            ttGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGTATTTTg                                                  >  1:5878672/1‑93 (MQ=255)

                                             tGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGc                                                 >  1:2401683/1‑93 (MQ=255)

                                                tGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTTAGTTCACAGGCCCCCAGAATTTTGCCCa                                              >  2:4136599/1‑93 (MQ=255)

                                                tGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCa                                              <  2:2100210/93‑1 (MQ=255)

                                                 ggCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCAt                                             >  1:353849/1‑93 (MQ=255)

                                                   cAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCAttt                                           <  1:5244975/93‑1 (MQ=255)

                                                    aaTTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTc                                          >  2:3447415/1‑93 (MQ=255)

                                                     aTTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTcc                                         <  2:1526732/93‑1 (MQ=255)

                                                      ttGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCa                                        <  1:465561/93‑1 (MQ=255)

                                                           ggAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCg                                   >  2:3763640/1‑93 (MQ=255)

                                                           ggAGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCg                                   >  1:4257115/1‑93 (MQ=255)

                                                            gAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGt                                  >  2:2601754/1‑93 (MQ=255)

                                                             aGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGtt                                 <  2:6241050/93‑1 (MQ=255)

                                                             aGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGtt                                 <  2:197454/93‑1 (MQ=255)

                                                             aGTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGtt                                 >  1:2980214/1‑93 (MQ=255)

                                                             aGTGACAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGtt                                 <  1:1696184/93‑1 (MQ=255)

                                                              gTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTg                                >  1:5681196/1‑93 (MQ=255)

                                                              gTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTg                                >  2:473665/1‑93 (MQ=255)

                                                              gTGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTg                                <  1:4222491/93‑1 (MQ=255)

                                                               tGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGa                               <  1:3104009/93‑1 (MQ=255)

                                                               tGGCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGa                               <  2:2511492/93‑1 (MQ=255)

                                                                ggCAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAg                              <  1:6437237/93‑1 (MQ=255)

                                                                 gCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGt                             <  2:547500/93‑1 (MQ=255)

                                                                  cAAAATTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGtt                            <  2:3637732/93‑1 (MQ=255)

                                                                   aaaaTTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTc                           >  1:885094/1‑93 (MQ=255)

                                                                   aaaaTTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTc                           >  2:3039153/1‑93 (MQ=255)

                                                                    aaaTTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTca                          >  1:4852978/1‑93 (MQ=255)

                                                                     aaTTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTcat                         <  2:644747/93‑1 (MQ=255)

                                                                      aTTCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGGGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTcatc                        >  1:1457633/1‑93 (MQ=255)

                                                                       ttCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTcatcc                       >  1:4202485/1‑93 (MQ=255)

                                                                       ttCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTcatcc                       <  2:5420506/93‑1 (MQ=255)

                                                                        tCATCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTcatcca                      >  1:6365631/1‑93 (MQ=255)

                                                                           tCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCa                   <  1:5525629/93‑1 (MQ=255)

                                                                           tCGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCa                   >  2:2443627/1‑93 (MQ=255)

                                                                            cGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCAc                  >  2:880993/1‑93 (MQ=255)

                                                                            cGAGATAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCAc                  <  1:4022975/93‑1 (MQ=255)

                                                                             gagaTAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACt                 >  2:465274/1‑93 (MQ=255)

                                                                              agaTAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa                >  2:1367912/1‑93 (MQ=255)

                                                                              agaTAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa                <  1:2291636/93‑1 (MQ=255)

                                                                                aTAATGGCAGAGTGCAGTCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACg              <  2:692259/93‑1 (MQ=255)

                                                                                aTAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACg              <  1:924726/93‑1 (MQ=255)

                                                                                aTAATGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACg              <  1:6312535/93‑1 (MQ=255)

                                                                                    tGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGt          <  2:5028015/93‑1 (MQ=255)

                                                                                    tGGCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGt          >  1:3637583/1‑93 (MQ=255)

                                                                                     ggCAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTa         >  1:1756652/1‑93 (MQ=255)

                                                                                      gCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTaa        >  1:5863775/1‑93 (MQ=255)

                                                                                       cAGAGCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCTCATTTCCAGATCGCTGAGTTCATCCATCACTACGTGGTAAc       >  2:5768135/1‑93 (MQ=255)

                                                                                        agagCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACg      >  2:1809268/1‑93 (MQ=255)

                                                                                        agagCGCAGGCGCGTACCGACGGTGAGTTCAAAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACATGGTAACg      >  1:2350335/1‑93 (MQ=255)

                                                                                        agagCGCAGGCGCGTACCGACGGTGAGATCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGGTGAGTTCATCCATCACTACGTGATAACg      >  1:5131799/1‑93 (MQ=255)

                                                                                         gagCGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACgg     >  2:5747/1‑93 (MQ=255)

GTAGTCCCAGCTGCTGCATAATCCCGGCGGATTTATGTTCGTAGTTGATGGCAATTGCCGGAGTGGCAAAATTCATCGAGATAATGGCAGAGTGCAGGCGCGTACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCA  >  NC_000913/2115325‑2115509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`