BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,179,549	T→C	L145P (CTG→CCG)	b2100 →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,179,549	0	T	C	86.2%	609.6 / 74.8	232	L145P (CTG→CCG)	b2100	putative kinase
Reads supporting (aligned to +/- strand): ref base T (12/20); new base C (95/105); total (107/125)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 240 total aligned reads displayed.

GGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA  >  NC_000913/2179457‑2179638

  aTGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGc                                                                                         <  2:4210960/93‑1 (MQ=255)

   tGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGct                                                                                        <  1:3949341/93‑1 (MQ=255)

   tGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGct                                                                                        <  1:2673686/93‑1 (MQ=255)

    ggCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGctc                                                                                       <  2:1975229/93‑1 (MQ=255)

    ggCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGctc                                                                                       <  2:1058578/93‑1 (MQ=255)

       gAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTAt                                                                                    >  1:3427898/1‑93 (MQ=255)

       gAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCTCTCTAt                                                                                    >  2:4625657/1‑93 (MQ=255)

        aaCGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAtt                                                                                   >  2:4098813/1‑93 (MQ=255)

          cGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAtttt                                                                                 <  1:1007615/93‑1 (MQ=255)

           gCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAttttt                                                                                >  1:3338334/1‑93 (MQ=255)

             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTcc                                                                              <  1:218891/93‑1 (MQ=255)

             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAttttttc                                                                              >  2:5986183/1‑91 (MQ=255)

             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              >  2:4462244/1‑93 (MQ=255)

             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              <  1:6407990/93‑1 (MQ=255)

             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              <  2:1092723/93‑1 (MQ=255)

              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCg                                                                             <  1:1538062/93‑1 (MQ=255)

              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:5270697/1‑93 (MQ=255)

              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:2506264/1‑93 (MQ=255)

              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:3498062/1‑93 (MQ=255)

               ttttATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCgg                                                                            >  1:3546878/1‑93 (MQ=255)

                tttATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGt                                                                           >  2:4963383/1‑93 (MQ=255)

                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:1505542/93‑1 (MQ=255)

                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:1952829/93‑1 (MQ=255)

                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:3145823/93‑1 (MQ=255)

                     gTCATTCAGCGGTGTTGAATATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCa                                                                      >  1:6481326/1‑93 (MQ=255)

                     gTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCa                                                                      >  1:1391897/1‑93 (MQ=255)

                       cATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAc                                                                    <  2:4058022/93‑1 (MQ=255)

                       cATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAc                                                                    >  1:3142636/1‑93 (MQ=255)

                        aTTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACt                                                                   <  1:3773833/93‑1 (MQ=255)

                        aTTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACt                                                                   <  1:4940706/93‑1 (MQ=255)

                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  <  1:5929680/93‑1 (MQ=255)

                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  <  1:4821347/93‑1 (MQ=255)

                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  >  2:1534149/1‑93 (MQ=255)

                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  >  2:4783965/1‑93 (MQ=255)

                            aGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGcc                                                               >  2:3794495/1‑93 (MQ=255)

                             gCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCt                                                              >  1:787570/1‑93 (MQ=255)

                             gCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCt                                                              <  1:1343700/93‑1 (MQ=255)

                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGGGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            >  2:5409446/1‑93 (MQ=255)

                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            <  2:3311328/93‑1 (MQ=255)

                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            <  1:6336033/93‑1 (MQ=255)

                                gtgtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGc                                                           <  1:6293703/93‑1 (MQ=255)

                                  gtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGccc                                                         <  2:6197627/93‑1 (MQ=255)

                                  gtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGccc                                                         <  2:2862229/93‑1 (MQ=255)

                                      aaaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcg                                                     <  2:3399709/93‑1 (MQ=255)

                                      aaaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcg                                                     >  2:6495710/1‑93 (MQ=255)

                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    >  2:87796/1‑93 (MQ=255)

                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    <  2:616276/93‑1 (MQ=255)

                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    >  1:5238071/1‑93 (MQ=255)

                                        aaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTACGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcggc                                                   >  1:541114/1‑93 (MQ=255)

                                           cAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGaa                                                <  1:6643011/93‑1 (MQ=255)

                                           cAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGaa                                                >  1:3301428/1‑93 (MQ=255)

                                            aGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAAt                                               <  2:5335085/93‑1 (MQ=255)

                                               ggAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGt                                            >  2:1025400/1‑93 (MQ=255)

                                                gAATCGCCAGTGGCTGGCGGGATTAACCGTTGCGCCTGGCAACCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:1934994/93‑1 (MQ=255)

                                                gAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:1325957/93‑1 (MQ=255)

                                                gAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:6303174/93‑1 (MQ=255)

                                                 aaTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTa                                          >  1:4161198/1‑93 (MQ=255)

                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         <  1:5980039/93‑1 (MQ=255)

                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         >  2:2465418/1‑93 (MQ=255)

                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         <  1:6376765/93‑1 (MQ=255)

                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  2:1875696/93‑1 (MQ=255)

                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  1:2080745/93‑1 (MQ=255)

                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  2:1747411/93‑1 (MQ=255)

                                                    cGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTg                                       <  2:5849316/93‑1 (MQ=255)

                                                    cGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTg                                       >  2:1805032/1‑93 (MQ=255)

                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:771235/93‑1 (MQ=255)

                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  1:5723971/93‑1 (MQ=255)

                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:576491/93‑1 (MQ=255)

                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:4758143/93‑1 (MQ=255)

                                                         gTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATg                                  >  2:5708482/1‑93 (MQ=255)

                                                             ctggcGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTg                              >  2:3033375/1‑93 (MQ=255)

                                                              gggcGCTATTCCCCGTTGCGTCTGGCAGCC‑GCTCTATTTTTCGGTTTATCACCT‑GCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGaa                           <  1:466746/92‑1 (MQ=255)

                                                               ggcGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGa                            >  2:2137586/1‑93 (MQ=255)

                                                                gcgcGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGaa                           <  1:2970940/93‑1 (MQ=255)

                                                                  gcgATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAaga                         <  1:2443026/93‑1 (MQ=255)

                                                                    gATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGc                       >  2:5548030/1‑93 (MQ=255)

                                                                    gATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGc                       >  2:1270077/1‑93 (MQ=255)

                                                                     atgaaCCGTTGCGCCGGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCt                      <  1:4200370/90‑1 (MQ=255)

                                                                     aTTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCt                      >  1:513985/1‑93 (MQ=255)

                                                                       tAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGc                    <  1:3090040/93‑1 (MQ=255)

                                                                        aaCCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTgga                   >  1:2926396/1‑91 (MQ=255)

                                                                        aaCCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCa                   <  1:2463110/93‑1 (MQ=255)

                                                                           cGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGa                <  1:4692438/93‑1 (MQ=255)

                                                                            gTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               >  2:5123940/1‑93 (MQ=255)

                                                                            gTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               <  1:6078906/93‑1 (MQ=255)

                                                                            gTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               >  1:1965164/1‑93 (MQ=255)

                                                                             ttGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACg              <  1:5367683/93‑1 (MQ=255)

                                                                                cgcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGa           >  1:4957140/1‑93 (MQ=255)

                                                                                cgcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGa           <  1:4313787/93‑1 (MQ=255)

                                                                                 gcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAc          <  2:2707554/93‑1 (MQ=255)

                                                                                 gcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAc          <  2:5082717/93‑1 (MQ=255)

                                                                                   cTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGGATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGc        >  2:26434/1‑93 (MQ=255)

                                                                                    tGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGcc       <  1:5553977/93‑1 (MQ=255)

                                                                                     ggCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCg      <  1:2908907/93‑1 (MQ=255)

                                                                                     ggCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCg      <  1:4740867/93‑1 (MQ=255)

                                                                                      gCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGt     >  2:2328919/1‑93 (MQ=255)

                                                                                        aGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGttt   >  2:3836314/1‑93 (MQ=255)

                                                                                        aGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAAGCCGttt   >  1:676929/1‑93 (MQ=255)

                                                                                         gCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTa  >  2:6477224/1‑93 (MQ=255)

                                                                                         gCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTa  >  1:3488869/1‑93 (MQ=255)

GGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA  >  NC_000913/2179457‑2179638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`