BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,793	T→C	T333A (ACG→GCG)	cirA ←	CirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,793	0	T	C	86.9%	671.1 / 83.2	252	T333A (ACG→GCG)	cirA	CirA
Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (102/117); total (120/132)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

Only 100 of 259 total aligned reads displayed.

CGTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAGGGTTT  >  NC_000913/2243703‑2243884

cgTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTga                                                                                           <  1:4030205/93‑1 (MQ=255)

cgTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCga                                                                                           <  1:4076090/93‑1 (MQ=255)

cgTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCga                                                                                           <  1:50609/93‑1 (MQ=255)

cgTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCga                                                                                           <  2:5953050/93‑1 (MQ=255)

cgTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCga                                                                                           <  2:5667842/93‑1 (MQ=255)

     tggctggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAAc                                                                                      <  1:3816027/93‑1 (MQ=255)

     tggctggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAAc                                                                                      <  2:6262509/93‑1 (MQ=255)

     tggctggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAAc                                                                                      >  1:5572319/1‑93 (MQ=255)

         tggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGAt                                                                                  >  1:5118924/1‑93 (MQ=255)

          ggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCGCCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGAtt                                                                                 <  2:3730517/93‑1 (MQ=255)

          ggcAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGGTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGAtt                                                                                 >  2:2425602/1‑93 (MQ=255)

             aGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAAt                                                                              <  2:3763596/93‑1 (MQ=255)

             aGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAAt                                                                              <  2:2236758/93‑1 (MQ=255)

             aGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAAt                                                                              >  2:1033439/1‑93 (MQ=255)

              gACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATc                                                                             <  2:3210093/93‑1 (MQ=255)

              gACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATc                                                                             >  1:6460169/1‑93 (MQ=255)

               gcGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCg                                                                            <  1:6257738/92‑1 (MQ=255)

               aCGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGACACTGATTAATCg                                                                            >  2:3416717/1‑93 (MQ=255)

                cGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGc                                                                           <  2:1896971/93‑1 (MQ=255)

                cGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGc                                                                           >  1:1812380/1‑93 (MQ=255)

                 gTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGcc                                                                          >  2:6445988/1‑93 (MQ=255)

                   tttGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGt                                                                        <  2:4720571/93‑1 (MQ=255)

                    ttGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTc                                                                       <  2:4427817/93‑1 (MQ=255)

                       gAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGc                                                                    <  2:1422353/93‑1 (MQ=255)

                        aGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCg                                                                   >  1:2556925/1‑93 (MQ=255)

                         gCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCgg                                                                  >  1:731763/1‑93 (MQ=255)

                         gCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCgg                                                                  >  2:4243414/1‑93 (MQ=255)

                          cTGGTGCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGc                                                                 <  2:750836/93‑1 (MQ=255)

                            ggTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCaa                                                               <  1:3646352/93‑1 (MQ=255)

                             gTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAAc                                                              <  2:4813049/93‑1 (MQ=255)

                              ttCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACg                                                             >  1:1273938/1‑93 (MQ=255)

                                cccccGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACgtg                                                           <  2:5781797/93‑1 (MQ=255)

                                cccccGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGGCGTCAGCGGCAACgtg                                                           >  1:5951804/1‑93 (MQ=255)

                                 ccccGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACgtgt                                                          >  1:5374769/1‑93 (MQ=255)

                                  cccGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTa                                                         <  1:2370387/93‑1 (MQ=255)

                                   ccGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTAt                                                        >  1:106372/1‑93 (MQ=255)

                                   ccGGTCAGGTTCACCGCATCGCTAAGTTTGGCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTAt                                                        <  2:5427479/93‑1 (MQ=255)

                                    cGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTAtt                                                       <  2:6006253/93‑1 (MQ=255)

                                     ggTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTAttt                                                      <  2:231333/93‑1 (MQ=255)

                                     ggTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTAttt                                                      >  2:6586668/1‑93 (MQ=255)

                                      gTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACTGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTg                                                     >  2:431388/1‑93 (MQ=255)

                                      gTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTg                                                     >  2:4530058/1‑93 (MQ=255)

                                      gTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTg                                                     >  2:1544372/1‑93 (MQ=255)

                                      gTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTg                                                     >  1:3340959/1‑93 (MQ=255)

                                       tCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGc                                                    <  2:5077451/93‑1 (MQ=255)

                                       tCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGc                                                    >  1:390159/1‑93 (MQ=255)

                                        cAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGcc                                                   >  1:6598667/1‑93 (MQ=255)

                                         aGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCg                                                  >  1:3176580/1‑93 (MQ=255)

                                          ggTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGt                                                 >  1:1115514/1‑93 (MQ=255)

                                             tCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGa                                              <  2:1886526/93‑1 (MQ=255)

                                             tCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGa                                              >  2:5196754/1‑93 (MQ=255)

                                              cACCGCATCGCTAAGTTTGTCGTGACGCCATTCGCCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGAc                                             <  1:5744709/93‑1 (MQ=255)

                                               cccGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGAcc                                            <  1:3705062/92‑1 (MQ=255)

                                                ccGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCg                                           <  1:3269828/93‑1 (MQ=255)

                                                ccGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCg                                           >  1:5199272/1‑93 (MQ=255)

                                                  gCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTa                                         <  1:2163329/93‑1 (MQ=255)

                                                   cATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTAt                                        <  2:3827033/93‑1 (MQ=255)

                                                   cATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTAt                                        >  1:4688943/1‑93 (MQ=255)

                                                    aTCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTAtt                                       <  2:1147463/93‑1 (MQ=255)

                                                       gCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCt                                    <  1:5802455/93‑1 (MQ=255)

                                                        cTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCtt                                   >  2:5268592/1‑93 (MQ=255)

                                                        cTAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCtt                                   >  1:3953505/1‑93 (MQ=255)

                                                         tAAGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCttt                                  <  1:4321016/93‑1 (MQ=255)

                                                           aGTTTGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCg                                >  1:1893905/1‑93 (MQ=255)

                                                             tttGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGa                              >  1:3015463/1‑93 (MQ=255)

                                                              ttGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGaa                             <  2:5616222/93‑1 (MQ=255)

                                                              ttGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGaa                             >  2:805465/1‑93 (MQ=255)

                                                               tGTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAg                            <  1:42658/93‑1 (MQ=255)

                                                                gTCGTGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGt                           <  2:447205/93‑1 (MQ=255)

                                                                    tGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATc                       <  1:2360675/93‑1 (MQ=255)

                                                                    tGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATc                       <  2:2799156/93‑1 (MQ=255)

                                                                    tGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATc                       <  2:3630102/93‑1 (MQ=255)

                                                                    tGACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATc                       >  2:1346739/1‑93 (MQ=255)

                                                                     gACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCg                      >  2:1770235/1‑93 (MQ=255)

                                                                     gACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCg                      <  1:3855605/93‑1 (MQ=255)

                                                                     gACGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCg                      <  2:2611358/93‑1 (MQ=255)

                                                                      aCGCCATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCgg                     <  1:2584240/93‑1 (MQ=255)

                                                                       cGCCAGTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCggg                    <  1:288069/93‑1 (MQ=255)

                                                                          cATTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGtctg                 >  1:1453906/1‑93 (MQ=255)

                                                                           aTTCACCGCCAACGGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGgctgc                <  2:4099564/93‑1 (MQ=255)

                                                                           aTTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGgctgc                >  2:2933191/1‑93 (MQ=255)

                                                                           aTTCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGgctgc                >  2:767022/1‑93 (MQ=255)

                                                                            ttCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGgctgct               >  1:1976059/1‑93 (MQ=255)

                                                                            ttCACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGgctgct               <  1:2470226/93‑1 (MQ=255)

                                                                              cACCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGt             <  1:358314/93‑1 (MQ=255)

                                                                               aCCGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGtt            <  1:6015110/93‑1 (MQ=255)

                                                                                ccGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTg           >  2:1972908/1‑93 (MQ=255)

                                                                                ccGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTg           >  1:4712832/1‑93 (MQ=255)

                                                                                ccGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTg           >  1:563477/1‑93 (MQ=255)

                                                                                ccGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTg           >  1:441505/1‑93 (MQ=255)

                                                                                ccGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTg           <  2:3506211/93‑1 (MQ=255)

                                                                                 cGCCAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGc          >  2:2144330/1‑93 (MQ=255)

                                                                                   ccAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCa        >  1:2913498/1‑93 (MQ=255)

                                                                                   ccAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCa        >  2:1669994/1‑93 (MQ=255)

                                                                                    cAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAg       >  1:6213451/1‑93 (MQ=255)

                                                                                    cAACCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAg       <  1:1638897/93‑1 (MQ=255)

                                                                                     aaCCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAgg      >  1:3660404/1‑93 (MQ=255)

                                                                                     aaCCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAgg      <  1:3086363/93‑1 (MQ=255)

                                                                                      aCCGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAggg     >  2:3117426/1‑93 (MQ=255)

                                                                                       ccGCGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAGGGt    <  2:3848867/93‑1 (MQ=255)

CGTACTGGCTGGCAGACGTTTTGGAGCTGGTTCCCCCGGTCAGGTTCACCGCATCGCTAAGTTTGTCGTGACGCCATTCACCGCCAACCGTGAGAAACTGATTAATCGCCGTCAGCGGCAACGTGTATTTGCCGTCGACCGTATTGCTTTCGGAAGTTATCGGGCTGCTGTTGCCAGGGTTT  >  NC_000913/2243703‑2243884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`