BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,062	C→T	F266F (TTC→TTT)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,062	0	C	T	84.7%	607.0 / 75.8	215	F266F (TTC→TTT)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (74/108); total (91/124)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGG  >  NC_000913/2264970‑2265154

 cACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                                                                                             <  1:1429947/93‑1 (MQ=255)

   cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTttgtc                                                                                           >  2:3355014/1‑93 (MQ=255)

    tggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTttgtcg                                                                                          >  2:2632690/1‑93 (MQ=255)

     ggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGc                                                                                         <  1:4820197/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       <  1:5171114/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       <  2:3080788/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGCTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       >  2:2305703/1‑93 (MQ=255)

        ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTgg                                                                                      <  2:1259922/93‑1 (MQ=255)

        ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTgg                                                                                      >  2:2549601/1‑93 (MQ=255)

            aTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGTAGATAACTTCGTCGCTGGGCGt                                                                                  <  2:5944878/93‑1 (MQ=255)

              cagccagtATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcc                                                                                <  2:5267182/93‑1 (MQ=255)

              cagccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGCAAGATAACTTCGTCGCTGGGCGTcc                                                                                <  1:2827336/93‑1 (MQ=255)

               agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcct                                                                               <  2:4044752/93‑1 (MQ=255)

               agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcct                                                                               <  2:6654602/93‑1 (MQ=255)

                 ccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgc                                                                             >  1:4390406/1‑93 (MQ=255)

                  cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgcc                                                                            <  1:5773977/93‑1 (MQ=255)

                  cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc                                                                            <  1:523877/93‑1 (MQ=255)

                   agcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgcct                                                                           <  1:5992466/93‑1 (MQ=255)

                    gcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg                                                                          <  2:3938586/93‑1 (MQ=255)

                     cATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTgg                                                                         <  2:3256780/93‑1 (MQ=255)

                     cATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTCTGTCGCTGGGCGTCCTGCCTgg                                                                         <  2:4744349/93‑1 (MQ=255)

                      aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  1:13219/93‑1 (MQ=255)

                      aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  2:4897517/93‑1 (MQ=255)

                      aTCGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  2:5591796/93‑1 (MQ=255)

                       tCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGa                                                                       <  1:1292805/93‑1 (MQ=255)

                       tCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGa                                                                       <  1:4889260/93‑1 (MQ=255)

                         tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAg                                                                     >  2:2533575/1‑93 (MQ=255)

                          tggggTGAATGATCCCTGCGCGATTAGCTGCGAGCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:4368506/92‑1 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:1144413/93‑1 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    >  1:2653689/1‑93 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:2045854/93‑1 (MQ=255)

                           ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTc                                                                   >  2:4250670/1‑93 (MQ=255)

                           ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTc                                                                   >  2:6532961/1‑93 (MQ=255)

                               tGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAg                                                               >  1:4667666/1‑93 (MQ=255)

                               tGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAg                                                               <  1:1694177/93‑1 (MQ=255)

                                gAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAgg                                                              >  2:3740228/1‑93 (MQ=255)

                                 aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt                                                             <  1:5095077/93‑1 (MQ=255)

                                    gATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTa                                                          <  1:1668996/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         <  2:3610729/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:6018741/1‑93 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         <  1:6366049/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:5259329/1‑93 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:3261447/1‑93 (MQ=255)

                                      tCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAGTTTGTCGCTGGGCGTCCTGCCTGGGAAGCCGCAGGTGGACa                                                        >  1:3509937/1‑93 (MQ=255)

                                      tCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACa                                                        >  2:4108338/1‑93 (MQ=255)

                                       cccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaa                                                       >  2:1780907/1‑93 (MQ=255)

                                        ccTGCGCTATTAGCTGCGACCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa                                                      <  2:5650392/93‑1 (MQ=255)

                                         cTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAAt                                                     >  1:5548892/1‑93 (MQ=255)

                                            cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGt                                                  >  2:5312588/1‑93 (MQ=255)

                                            cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGt                                                  >  1:3164473/1‑93 (MQ=255)

                                              cgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGGACAAATGGTGa                                                >  2:5106473/1‑93 (MQ=255)

                                               gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGaa                                               <  2:1045690/93‑1 (MQ=255)

                                               gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGaa                                               <  2:6078831/93‑1 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  1:4843812/1‑93 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  2:1620219/1‑93 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  2:1848132/1‑93 (MQ=255)

                                                 ttAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatg                                             >  1:5512875/1‑93 (MQ=255)

                                                  tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatga                                            <  1:6206659/93‑1 (MQ=255)

                                                  tAGCTCCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatga                                            >  1:2807597/1‑93 (MQ=255)

                                                   aGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatgat                                           <  2:918720/93‑1 (MQ=255)

                                                    gCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatgatg                                          <  1:2260866/93‑1 (MQ=255)

                                                       gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTcc                                       >  1:3288054/1‑93 (MQ=255)

                                                         gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTg                                     >  2:4613972/1‑93 (MQ=255)

                                                         gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTg                                     <  2:3770567/93‑1 (MQ=255)

                                                           aCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGcc                                   <  2:2855580/93‑1 (MQ=255)

                                                            ccGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCa                                  >  1:4585069/1‑93 (MQ=255)

                                                            ccGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCa                                  <  1:5624198/93‑1 (MQ=255)

                                                             cGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAt                                 >  1:6061853/1‑93 (MQ=255)

                                                             cGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAt                                 <  2:4986318/93‑1 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                >  1:3702118/1‑93 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                <  1:2380953/93‑1 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                >  1:4123039/1‑93 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                <  2:3811759/93‑1 (MQ=255)

                                                               tttATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATgg                               >  2:4529305/1‑93 (MQ=255)

                                                                ttATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATggg                              <  2:2794533/93‑1 (MQ=255)

                                                                ttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATggg                              <  1:4308803/93‑1 (MQ=255)

                                                                 tATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGa                             <  2:6497709/93‑1 (MQ=255)

                                                                  aTCCAGTGTGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGaa                            <  1:2910100/93‑1 (MQ=255)

                                                                  aTCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGaa                            <  2:4959733/93‑1 (MQ=255)

                                                                   tCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAag                           >  2:1351356/1‑93 (MQ=255)

                                                                     cAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGCGTACAAATGGTGAATGATGTACTGCCATGGGAagag                         >  1:2937285/1‑93 (MQ=255)

                                                                      aGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAgaga                        <  1:6119149/93‑1 (MQ=255)

                                                                       gtgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAt                       <  2:878734/93‑1 (MQ=255)

                                                                        tgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATg                      <  2:1771983/93‑1 (MQ=255)

                                                                        tgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATg                      >  2:1717364/1‑93 (MQ=255)

                                                                         gggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGa                     <  2:5167677/93‑1 (MQ=255)

                                                                         gggtggTGGAAGATAACTTTGTCGCGGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGa                     <  1:695807/93‑1 (MQ=255)

                                                                            tggtggAAGATAGCTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAAc                  <  2:6240/93‑1 (MQ=255)

                                                                            tggtggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAAc                  >  1:4350163/1‑93 (MQ=255)

                                                                             ggtggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACt                 <  1:2533218/93‑1 (MQ=255)

                                                                               tggAACATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGCGAATGATGTCCTGCCATGGGAAGAGATGAAACTGc               >  1:2229241/1‑93 (MQ=255)

                                                                                ggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  1:586939/1‑93 (MQ=255)

                                                                                ggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  2:4332087/1‑93 (MQ=255)

                                                                                ggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  1:3979176/1‑93 (MQ=255)

                                                                                ggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              <  1:1078402/93‑1 (MQ=255)

                                                                                  aaGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGa            <  2:6629375/93‑1 (MQ=255)

                                                                                       aaCTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt       <  1:830781/93‑1 (MQ=255)

                                                                                       aaCTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt       >  2:5775481/1‑93 (MQ=255)

                                                                                           tcgtcgCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg   <  1:5899798/93‑1 (MQ=255)

                                                                                            cgtcgCTGGGGGTGCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg  <  1:1884051/93‑1 (MQ=255)

TCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGG  >  NC_000913/2264970‑2265154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`