BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,359	T→C	Y85C (TAC→TGC)	yfbB ←	hyptothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,359	0	T	C	88.9%	617.2 / 51.6	225	Y85C (TAC→TGC)	yfbB	hyptothetical protein
Reads supporting (aligned to +/- strand): ref base T (14/11); new base C (105/95); total (119/106)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 230 total aligned reads displayed.

AGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCG  >  NC_000913/2375269‑2375449

aGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAc                                                                                          <  2:1488204/93‑1 (MQ=255)

aGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAc                                                                                          <  2:2292011/93‑1 (MQ=255)

aGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAACCGAGCAc                                                                                          >  2:4294004/1‑93 (MQ=255)

  cccgGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACTAAGCGAGCAccc                                                                                        <  1:5309219/93‑1 (MQ=255)

  ccccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAccc                                                                                        >  1:4252952/1‑93 (MQ=255)

    ccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCcca                                                                                      >  1:4220777/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCNTCCACCAAGCGAGCACCccncc                                                                                    >  2:5214942/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCccacc                                                                                    >  1:6324275/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCccaca                                                                                    >  1:4240866/1‑92 (MQ=255)

        aTGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccag                                                                                  <  2:1044343/93‑1 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  1:5058277/1‑93 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  2:1125272/1‑93 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  1:3378014/1‑93 (MQ=255)

             cGCCTTCGACAATACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa                                                                             <  2:5003886/93‑1 (MQ=255)

             cGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa                                                                             >  2:362246/1‑93 (MQ=255)

              gCCTTCTAAAATACCCCCACAAAGCCCCGCCGGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGaa                                                                            <  2:5916069/93‑1 (MQ=255)

              gCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGaa                                                                            <  1:2735169/93‑1 (MQ=255)

               ccTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAg                                                                           >  2:3773439/1‑93 (MQ=255)

               ccTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAg                                                                           >  2:1373793/1‑93 (MQ=255)

                 ttCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTc                                                                         <  1:5934150/93‑1 (MQ=255)

                 ttCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTc                                                                         >  2:4485141/1‑93 (MQ=255)

                    gACAAAAACCCCAAAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAg                                                                      <  2:3269091/93‑1 (MQ=255)

                    aaCAAAACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAg                                                                      <  2:657666/92‑1 (MQ=255)

                      cAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGa                                                                    <  2:1508074/93‑1 (MQ=255)

                       aaTACCCCCACAAGCCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAt                                                                   <  1:257353/93‑1 (MQ=255)

                       aaTACCCCCACAACGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAt                                                                   <  2:2721496/93‑1 (MQ=255)

                        aTAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtg                                                                  >  2:5468429/1‑93 (MQ=255)

                         tacccccccAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 <  2:6016400/90‑1 (MQ=255)

                         tAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 >  1:3327199/1‑93 (MQ=255)

                         aacccccACAAGGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 <  2:187846/90‑1 (MQ=255)

                          acccccACAAGGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgtt                                                                <  1:2912881/91‑1 (MQ=255)

                             cccACAAATCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGta                                                             >  1:6481149/1‑93 (MQ=255)

                               cacaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaac                                                           <  2:577597/93‑1 (MQ=255)

                                acaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGtaact                                                          >  1:3200023/1‑93 (MQ=255)

                                acaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaact                                                          >  2:2131104/1‑93 (MQ=255)

                                 caAGGCCCGGCGAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaacta                                                         <  2:2180707/93‑1 (MQ=255)

                                   acgCCCCGCCAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaac                                                       <  1:3034656/91‑1 (MQ=255)

                                   aacccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaac                                                       <  2:1710804/90‑1 (MQ=255)

                                    acccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  1:131655/91‑1 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  2:1802049/93‑1 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      >  2:6558966/1‑93 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  2:1016413/93‑1 (MQ=255)

                                      ccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCaa                                                    <  1:2367321/93‑1 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCATGGATGTTGTAACTAACCAAgg                                                  >  1:6098487/1‑93 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAgg                                                  >  2:3546266/1‑93 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAgg                                                  <  1:3972525/93‑1 (MQ=255)

                                         cGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGt                                                 <  1:2317262/93‑1 (MQ=255)

                                            cAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGtttt                                              <  2:3132124/93‑1 (MQ=255)

                                            cAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGtttt                                              <  1:2540161/93‑1 (MQ=255)

                                             aGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTa                                             <  2:911087/93‑1 (MQ=255)

                                              gCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTAc                                            >  2:5403320/1‑93 (MQ=255)

                                                cccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGc                                          >  2:680588/1‑93 (MQ=255)

                                                 ccTGGCAAGCCGCCATCATCGCCGCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCa                                         <  2:4388746/93‑1 (MQ=255)

                                                  cTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAg                                        >  1:2012692/1‑93 (MQ=255)

                                                    ggCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      >  1:1926866/1‑93 (MQ=255)

                                                    ggCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      >  1:1401112/1‑93 (MQ=255)

                                                    gccAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      <  2:4929265/91‑1 (MQ=255)

                                                     gCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTaa                                     <  2:2896184/93‑1 (MQ=255)

                                                       aaGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGt                                   <  1:4580408/93‑1 (MQ=255)

                                                        aGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGtg                                  >  1:1059390/1‑92 (MQ=255)

                                                        aGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTc                                  <  1:6246703/93‑1 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             <  2:5908723/93‑1 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             >  2:2536569/1‑93 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             <  1:2649499/93‑1 (MQ=255)

                                                              catcatCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAc                            >  2:2061259/1‑93 (MQ=255)

                                                              catcatCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGCTTTACGCAGTAAGTCGGTGAc                            >  1:3921381/1‑93 (MQ=255)

                                                               atcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAca                           <  1:3770208/93‑1 (MQ=255)

                                                               atcatcGCCACCCGTCCAACAAGCGAGCACCCCACCAGACAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAca                           >  1:2156149/1‑93 (MQ=255)

                                                                tcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcat                          >  1:5475424/1‑93 (MQ=255)

                                                                tcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcat                          <  1:4137554/93‑1 (MQ=255)

                                                                   tcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatcat                       >  1:4477916/1‑93 (MQ=255)

                                                                    cGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACatcatc                      <  2:4175314/93‑1 (MQ=255)

                                                                      ccACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCaa                    <  1:6045808/93‑1 (MQ=255)

                                                                        ccccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  <  1:1198552/92‑1 (MQ=255)

                                                                        aCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  >  1:5881360/1‑93 (MQ=255)

                                                                        aCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  >  2:1693581/1‑93 (MQ=255)

                                                                          ccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  2:3015580/93‑1 (MQ=255)

                                                                          ccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  1:3685605/93‑1 (MQ=255)

                                                                          ccGGCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  2:4444943/93‑1 (MQ=255)

                                                                           cGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCa               <  2:3084605/93‑1 (MQ=255)

                                                                           cGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCa               <  1:6185796/93‑1 (MQ=255)

                                                                            gTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt              >  1:5552453/1‑93 (MQ=255)

                                                                             tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             <  2:6269857/93‑1 (MQ=255)

                                                                             tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             >  1:3531940/1‑93 (MQ=255)

                                                                             tCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             <  1:3986785/93‑1 (MQ=255)

                                                                             tCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             >  1:4852134/1‑93 (MQ=255)

                                                                               caccaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGa           >  2:4189867/1‑93 (MQ=255)

                                                                               caccaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGa           <  2:3994350/93‑1 (MQ=255)

                                                                                accaATCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTGTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          <  1:731709/93‑1 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  1:4436310/1‑93 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  2:162255/1‑93 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGGTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  1:4098352/1‑93 (MQ=255)

                                                                                  caAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGc        <  1:6640443/93‑1 (MQ=255)

                                                                                  caAGCGACCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGc        <  1:2893352/93‑1 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  2:743151/1‑93 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  1:1572263/1‑93 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  1:5511029/1‑93 (MQ=255)

                                                                                      cGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAAt    <  1:961596/93‑1 (MQ=255)

                                                                                       gAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATc   >  2:5107377/1‑93 (MQ=255)

                                                                                        aGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcg  >  2:2044387/1‑93 (MQ=255)

AGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCG  >  NC_000913/2375269‑2375449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`