BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,383,620	C→T	V41V (GTG→GTA)	yfbK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,383,620	0	C	T	90.0%	504.4 / 32.0	160	V41V (GTG→GTA)	yfbK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (7/9); new base T (58/86); total (65/95)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

Only 100 of 160 total aligned reads displayed.

TGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCA  >  NC_000913/2383530‑2383711

tGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTAc                                                                                           >  2:3990512/1‑93 (MQ=255)

 gAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACt                                                                                          <  1:1300178/93‑1 (MQ=255)

 gAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACt                                                                                          >  2:6009720/1‑93 (MQ=255)

  aaTATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACtt                                                                                         <  2:5630994/93‑1 (MQ=255)

  aaTATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACtt                                                                                         >  1:4598097/1‑93 (MQ=255)

    tatTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgc                                                                                       <  1:3219821/93‑1 (MQ=255)

    tatTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgc                                                                                       <  1:6025901/93‑1 (MQ=255)

    tatTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTtgc                                                                                       >  1:4102257/1‑93 (MQ=255)

    tatGGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgc                                                                                       <  1:4258544/93‑1 (MQ=255)

     atTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgct                                                                                      <  2:5973401/93‑1 (MQ=255)

     atTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTtgct                                                                                      <  1:1836855/93‑1 (MQ=255)

      ttgttgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgctg                                                                                     <  2:1933737/93‑1 (MQ=255)

       tgttgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgctgc                                                                                    <  1:1160633/93‑1 (MQ=255)

       tgttgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTtgctgc                                                                                    <  2:5016243/93‑1 (MQ=255)

          tgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGctct                                                                                 <  2:4400362/93‑1 (MQ=255)

            cACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCtgt                                                                               >  2:1554810/1‑93 (MQ=255)

             aCTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCtgtg                                                                              <  2:3085934/93‑1 (MQ=255)

               ttcttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTggg                                                                            >  2:4867478/1‑93 (MQ=255)

                tcttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGa                                                                           <  1:5535708/93‑1 (MQ=255)

                  ttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGt                                                                         <  1:1586831/93‑1 (MQ=255)

                  ttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGt                                                                         >  2:848423/1‑93 (MQ=255)

                    gCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTAc                                                                       >  1:6309563/1‑93 (MQ=255)

                    gCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTAc                                                                       <  2:4293905/93‑1 (MQ=255)

                    gCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTAc                                                                       <  2:5095621/93‑1 (MQ=255)

                    gCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTAc                                                                       <  2:815723/93‑1 (MQ=255)

                     cTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACt                                                                      <  1:2255402/93‑1 (MQ=255)

                      tGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTg                                                                     <  1:5997217/93‑1 (MQ=255)

                      tGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTg                                                                     >  1:604802/1‑93 (MQ=255)

                      tGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTg                                                                     <  2:2228093/93‑1 (MQ=255)

                         gCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgt                                                                  >  2:2738916/1‑93 (MQ=255)

                         gCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgt                                                                  >  2:1050181/1‑93 (MQ=255)

                          ccAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgtt                                                                 <  1:5172673/93‑1 (MQ=255)

                            aaGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgt                                                               >  1:636532/1‑93 (MQ=255)

                            aaGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgt                                                               >  1:4813530/1‑93 (MQ=255)

                            aaGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgt                                                               >  2:6113340/1‑93 (MQ=255)

                            aaGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgt                                                               >  1:3218658/1‑93 (MQ=255)

                             aGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgtt                                                              <  2:1721366/93‑1 (MQ=255)

                             aGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgtt                                                              <  1:2790037/93‑1 (MQ=255)

                             aGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgtt                                                              >  2:6545111/1‑93 (MQ=255)

                              ggCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgttg                                                             >  1:4934148/1‑93 (MQ=255)

                               gCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgttgt                                                            <  2:6605897/93‑1 (MQ=255)

                                cTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGgttgttgtt                                                           >  1:5658368/1‑93 (MQ=255)

                                  ttCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGc                                                         <  1:1909398/93‑1 (MQ=255)

                                     gcggcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGa                                                      <  1:805038/93‑1 (MQ=255)

                                     gcggcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGa                                                      >  1:6546848/1‑93 (MQ=255)

                                      cggcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGAc                                                     <  2:1261452/93‑1 (MQ=255)

                                        gcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACtt                                                   <  2:3446584/93‑1 (MQ=255)

                                          ggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTc                                                 <  1:3717388/93‑1 (MQ=255)

                                           gcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTcc                                                >  1:5288826/1‑93 (MQ=255)

                                            cggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCt                                               <  2:6096671/93‑1 (MQ=255)

                                             ggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCtt                                              >  2:5020577/1‑93 (MQ=255)

                                              gcgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTa                                             >  1:1085986/1‑93 (MQ=255)

                                               cgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAt                                            <  1:3113925/93‑1 (MQ=255)

                                               cgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAt                                            <  1:1007760/93‑1 (MQ=255)

                                               cgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAt                                            <  2:6353478/93‑1 (MQ=255)

                                               cgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAt                                            <  2:4482946/93‑1 (MQ=255)

                                               cgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAt                                            <  2:5888374/93‑1 (MQ=255)

                                                gcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAtt                                           >  1:66090/1‑93 (MQ=255)

                                                gcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTAtt                                           >  2:3287896/1‑93 (MQ=255)

                                                 cTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATtc                                          >  1:2370835/1‑93 (MQ=255)

                                                  tttGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATtct                                         <  2:5249975/93‑1 (MQ=255)

                                                   ttGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATtctc                                        <  2:4864899/93‑1 (MQ=255)

                                                    tGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCa                                       <  1:2598464/93‑1 (MQ=255)

                                                    tGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCa                                       <  1:4401548/93‑1 (MQ=255)

                                                     gCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGTTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAg                                      <  1:2465895/93‑1 (MQ=255)

                                                      cTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAgg                                     >  1:1019930/1‑93 (MQ=255)

                                                      cTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAgg                                     >  2:2864634/1‑93 (MQ=255)

                                                       tCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGt                                    >  2:3219578/1‑93 (MQ=255)

                                                        cAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGtt                                   <  1:4333688/93‑1 (MQ=255)

                                                          gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGttcc                                 <  1:3465505/93‑3 (MQ=255)

                                                          gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGc                                 <  2:5308903/93‑1 (MQ=255)

                                                          gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGc                                 >  1:5454386/1‑93 (MQ=255)

                                                          gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGc                                 >  2:5365202/1‑93 (MQ=255)

                                                          gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGc                                 <  2:4738679/93‑1 (MQ=255)

                                                           ccTCTTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCg                                >  1:3569273/1‑93 (MQ=255)

                                                            ctctTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCgg                               >  1:260033/1‑93 (MQ=255)

                                                              ctTTTATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGcc                             <  1:772637/93‑1 (MQ=255)

                                                               ttttATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGccc                            <  2:1764396/93‑1 (MQ=255)

                                                                tttATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCca                           >  2:964226/1‑93 (MQ=255)

                                                                tttATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCca                           >  1:3473808/1‑93 (MQ=255)

                                                                tttATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCca                           <  2:447288/93‑1 (MQ=255)

                                                                  tATTGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGGTGCGGCCcaca                         >  1:2803323/1‑93 (MQ=255)

                                                                     tGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATcc                      <  2:1918852/93‑1 (MQ=255)

                                                                     tGCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATcc                      <  1:6427150/93‑1 (MQ=255)

                                                                      gCAGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCt                     <  1:1082271/93‑1 (MQ=255)

                                                                        aGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGa                   <  1:3521562/93‑1 (MQ=255)

                                                                        aGCTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGa                   >  1:3899727/1‑93 (MQ=255)

                                                                          cTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACa                 >  2:4153819/1‑93 (MQ=255)

                                                                          cTTGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACa                 >  1:1995482/1‑93 (MQ=255)

                                                                           ttGCTGCGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACaa                >  1:1474680/1‑93 (MQ=255)

                                                                             gctgcGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACaaaa              <  1:5007229/93‑1 (MQ=255)

                                                                               tgcTCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCCGACAAAATc            <  1:3701309/93‑1 (MQ=255)

                                                                               tgcGCGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATc            <  2:3390168/93‑1 (MQ=255)

                                                                                gcgcGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCa           >  1:3991688/1‑93 (MQ=255)

                                                                                gcgcGGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCa           <  1:162483/93‑1 (MQ=255)

                                                                                   cgGCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAac        <  1:4920782/93‑1 (MQ=255)

                                                                                    ggCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAact       <  1:3796740/93‑1 (MQ=255)

                                                                                    ggCAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAact       <  2:1328178/93‑1 (MQ=255)

                                                                                      cAAGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAactac     <  1:6586099/93‑1 (MQ=255)

                                                                                       aaGTACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAactact    >  2:3898792/1‑93 (MQ=255)

TGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCA  >  NC_000913/2383530‑2383711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`