BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,601	T→C	T119A (ACG→GCG)	hscA ←	HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,601	0	T	C	99.6%	935.7 / ‑5.1	254	T119A (ACG→GCG)	hscA	HscA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (114/139); minor base A (1/0); total (115/139)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

Only 100 of 266 total aligned reads displayed.

GCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCA  >  NC_000913/2656511‑2656690

  tCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCa                                                                                       >  2:4509497/1‑93 (MQ=255)

  tCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCa                                                                                       <  1:1693174/93‑1 (MQ=255)

    gCCTGCCAGGGCTTCAGTTGCCCGCGTCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAat                                                                                     <  1:5359948/93‑1 (MQ=255)

    gCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAat                                                                                     <  2:1726132/93‑1 (MQ=255)

       tGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcat                                                                                  >  2:938710/1‑93 (MQ=255)

       tGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcat                                                                                  <  1:5256938/93‑1 (MQ=255)

       tGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcat                                                                                  <  2:5426796/93‑1 (MQ=255)

        gCCAGGTCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTGCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcatc                                                                                 <  1:5475753/93‑1 (MQ=255)

        gCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcatc                                                                                 <  2:1935935/93‑1 (MQ=255)

        gCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAatcatc                                                                                 <  2:2407349/93‑1 (MQ=255)

          cAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGGCCCGCCGCCGCTTCAATCATCgg                                                                               >  1:1839289/1‑93 (MQ=255)

          cAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCgg                                                                               >  1:1811364/1‑93 (MQ=255)

          cAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCgg                                                                               >  1:3156164/1‑93 (MQ=255)

           aGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGc                                                                              >  2:3472262/1‑93 (MQ=255)

           aGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGc                                                                              <  1:3898412/93‑1 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCggga                                                                             >  1:6438968/1‑91 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCa                                                                             >  2:4109261/1‑93 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCa                                                                             <  2:3332158/93‑1 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCa                                                                             >  2:67373/1‑93 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCa                                                                             >  1:1202569/1‑93 (MQ=255)

            gggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCa                                                                             <  1:4147128/93‑1 (MQ=255)

             ggCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAg                                                                            >  2:5226069/1‑93 (MQ=255)

              gCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAgg                                                                           <  1:6608442/93‑1 (MQ=255)

               gttCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGc                                                                          <  2:4817792/92‑1 (MQ=255)

               cTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGc                                                                          <  2:6401722/93‑1 (MQ=255)

               cTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGc                                                                          >  1:78419/1‑93 (MQ=255)

                 tCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCg                                                                        >  1:2150655/1‑93 (MQ=255)

                   aGTTGCCCGCGCCACCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGtt                                                                      <  1:5401410/93‑1 (MQ=255)

                    gTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGttt                                                                     >  1:699561/1‑93 (MQ=255)

                     ttGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGtttt                                                                    <  2:3824531/93‑1 (MQ=255)

                     ttGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGtttt                                                                    >  1:6195236/1‑93 (MQ=255)

                        cccGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGc                                                                 <  1:92343/93‑1 (MQ=255)

                          cgcgCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTg                                                               <  2:2882424/93‑1 (MQ=255)

                          cgcgCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTg                                                               <  1:5629022/93‑1 (MQ=255)

                            cgccgcCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGc                                                             <  1:4902186/93‑1 (MQ=255)

                            cgccgcCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGc                                                             >  2:2538203/1‑93 (MQ=255)

                             gccgccAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCCCTGGcc                                                            >  1:6372821/1‑93 (MQ=255)

                              ccgccAGTGCTTTGAGGATGTCCGCAGAAACGCGCACGGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCt                                                           <  1:441734/93‑1 (MQ=255)

                              ccgccAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCt                                                           <  2:5253266/93‑1 (MQ=255)

                               cgctAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTg                                                          <  1:1903319/93‑1 (MQ=255)

                               cgccAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTg                                                          >  1:1029468/1‑93 (MQ=255)

                                gccAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTgg                                                         >  1:3544408/1‑93 (MQ=255)

                                  cAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGaa                                                       >  1:2336469/1‑93 (MQ=255)

                                   aGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTGCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAAt                                                      <  1:6011499/93‑1 (MQ=255)

                                     tGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTg                                                    <  2:1262202/93‑1 (MQ=255)

                                      gCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGa                                                   <  2:3983498/93‑1 (MQ=255)

                                      gCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGa                                                   <  1:6089943/93‑1 (MQ=255)

                                       cTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGAt                                                  <  2:1990367/93‑1 (MQ=255)

                                       cTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGAt                                                  >  1:718771/1‑93 (MQ=255)

                                        tttGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATa                                                 <  1:5216558/93‑1 (MQ=255)

                                        tttGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATa                                                 >  1:295189/1‑93 (MQ=255)

                                        tttGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATa                                                 >  1:4799288/1‑93 (MQ=255)

                                         ttGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATaa                                                >  2:2822316/1‑93 (MQ=255)

                                         ttGAGGATGTCCGCAGAAACGCGCACCGGATTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATaa                                                <  1:3282523/93‑1 (MQ=255)

                                          tGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAg                                               >  1:1283549/1‑93 (MQ=255)

                                           gAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAgg                                              >  1:5692107/1‑93 (MQ=255)

                                            aGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGc                                             <  1:2887849/93‑1 (MQ=255)

                                             ggATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGTATTGATAAGGCa                                            >  2:5959368/1‑93 (MQ=255)

                                             ggATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCa                                            >  2:4226227/1‑93 (MQ=255)

                                             ggATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCa                                            >  2:515492/1‑93 (MQ=255)

                                                tGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGAt                                         <  1:6881/93‑1 (MQ=255)

                                                  tCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGc                                       <  1:4487574/93‑1 (MQ=255)

                                                     gCAGAAACGCGCACCGGGGTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGa                                    >  2:1201036/1‑93 (MQ=255)

                                                      cAGAACCGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGAt                                   <  2:190575/93‑1 (MQ=255)

                                                      cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGAt                                   <  1:4095824/93‑1 (MQ=255)

                                                      cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGAt                                   >  2:1612555/1‑93 (MQ=255)

                                                      cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGAt                                   <  2:5615984/93‑1 (MQ=255)

                                                         aaaCGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGc                                <  2:2181669/93‑1 (MQ=255)

                                                          aaCGCGCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCg                               >  1:4070291/1‑93 (MQ=255)

                                                            cgcgCACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGtt                             <  1:3729065/93‑1 (MQ=255)

                                                             gcgcACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTg                            <  2:6055315/93‑1 (MQ=255)

                                                             gcgcACCGGCTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTg                            <  2:3208670/93‑1 (MQ=255)

                                                              cgcACCGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGc                           >  2:502897/1‑93 (MQ=255)

                                                                 cccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTgg                        <  2:1312006/92‑1 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       <  1:2456638/93‑1 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       >  2:5303512/1‑93 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       <  2:747602/93‑1 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       >  1:4643658/1‑93 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       >  1:3915640/1‑93 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       >  1:6043373/1‑93 (MQ=255)

                                                                  ccGGGTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       >  2:1114546/1‑93 (MQ=255)

                                                                  ccGGGTTCAGCACCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGa                       <  2:4568697/93‑1 (MQ=255)

                                                                    gggTTCAGCGGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGata                     <  2:2945066/93‑1 (MQ=255)

                                                                     ggTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGatat                    >  1:343923/1‑93 (MQ=255)

                                                                     ggTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGatat                    >  1:3327039/1‑93 (MQ=255)

                                                                      gTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATc                   <  1:2094613/93‑1 (MQ=255)

                                                                      gTTCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATc                   <  2:4279516/93‑1 (MQ=255)

                                                                       ttCAGCAGCCCCGCCGCCGCTGCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATca                  <  2:1884417/93‑1 (MQ=255)

                                                                        tCAGCAGCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATcag                 >  2:459930/1‑93 (MQ=255)

                                                                         cagcagCCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATcagc                <  2:2024235/93‑1 (MQ=255)

                                                                           gcagcCCCGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATcagcca              <  1:1134777/93‑1 (MQ=255)

                                                                               ccccGCCGCCGCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCagcga          <  2:1471821/93‑1 (MQ=255)

                                                                                 ccgccgccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCagcgagc        >  1:3615347/1‑93 (MQ=255)

                                                                                 ccgccgccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCagcgagc        >  1:6575446/1‑93 (MQ=255)

                                                                                 ccgccgccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCagcgagc        >  2:2602090/1‑93 (MQ=255)

                                                                                     cgccgcTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTcc    >  1:2637955/1‑93 (MQ=255)

                                                                                      gccgCTTCAATCATCGGCAGGCCGTTTTCGCTTGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTacc   >  1:615336/1‑93 (MQ=255)

                                                                                      gccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTtcc   >  1:1397483/1‑93 (MQ=255)

                                                                                      gccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTccc   >  2:4884735/1‑93 (MQ=255)

                                                                                       ccgCTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCa  >  2:6092744/1‑93 (MQ=255)

GCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCA  >  NC_000913/2656511‑2656690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`