Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,658,670 T→C E294E (GAA→GAG)  b2530 ← putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,658,6700TC87.1% 731.4 / 87.3 272E294E (GAA→GAG) b2530putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (20/15);  new base C (119/118);  total (139/133)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 283 total aligned reads displayed.

CCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGT  >  NC_000913/2658578‑2658759
                                                                                            |                                                                                         
 cTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGtt                                                                                          >  2:4001494/1‑93 (MQ=255)
  tGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTc                                                                                         <  2:4743543/93‑1 (MQ=255)
  tGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTc                                                                                         >  2:4963893/1‑93 (MQ=255)
  tGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTc                                                                                         >  2:3794844/1‑93 (MQ=255)
   gaagaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTcc                                                                                        >  1:5993342/1‑93 (MQ=255)
   gaagaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTcc                                                                                        >  1:299298/1‑93 (MQ=255)
     agaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAg                                                                                      <  2:5779364/93‑1 (MQ=255)
         aCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCa                                                                                  >  2:6398505/1‑93 (MQ=255)
         aCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCa                                                                                  >  1:6055271/1‑93 (MQ=255)
           tGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCAcc                                                                                >  1:1598807/1‑93 (MQ=255)
           tGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCAcc                                                                                >  2:2813223/1‑93 (MQ=255)
           tGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCAcc                                                                                >  1:99981/1‑93 (MQ=255)
             cGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGt                                                                              >  1:6248818/1‑93 (MQ=255)
              gAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGtt                                                                             <  1:3838380/93‑1 (MQ=255)
              gAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGtt                                                                             <  2:5320213/93‑1 (MQ=255)
              gAGGTCTTTCAGCGCCATAATCAGCGACTCAACTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGtt                                                                             >  1:3326687/1‑93 (MQ=255)
               aGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTc                                                                            <  2:3867066/93‑1 (MQ=255)
                ggTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCa                                                                           >  2:2936009/1‑93 (MQ=255)
                 gTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAg                                                                          <  2:4671692/93‑1 (MQ=255)
                 gTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAg                                                                          >  1:4273891/1‑93 (MQ=255)
                  gcTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAgg                                                                         <  1:4532573/92‑1 (MQ=255)
                   cTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGt                                                                        >  2:5801290/1‑93 (MQ=255)
                    tttCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTa                                                                       >  1:1329130/1‑93 (MQ=255)
                     ttCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCGCCAGGTCACCGTTCAGGTaa                                                                      <  2:5059381/93‑1 (MQ=255)
                     tgcAGCGCCATAATCAGCGACTCCCCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTaa                                                                      <  1:6421268/91‑1 (MQ=255)
                        aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAAct                                                                   >  2:4912497/1‑93 (MQ=255)
                        aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAAct                                                                   >  1:5184696/1‑93 (MQ=255)
                        aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAAct                                                                   >  1:437318/1‑93 (MQ=255)
                          ctccATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAActtc                                                                 <  1:3874174/91‑1 (MQ=255)
                          cgcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAActtc                                                                 <  1:148113/93‑1 (MQ=255)
                            ccATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCCCCGTGTTCCAGGTCCCCGTTCAGGTAAActtctt                                                               <  1:233028/93‑1 (MQ=255)
                             cATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTACACttcttc                                                              <  1:6541844/93‑1 (MQ=255)
                             cATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACttcttc                                                              <  2:1196851/93‑1 (MQ=255)
                               tAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGa                                                            <  2:2710125/93‑1 (MQ=255)
                               tAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGa                                                            <  1:2387794/93‑1 (MQ=255)
                                    aGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCt                                                       <  1:551729/93‑1 (MQ=255)
                                    aGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCt                                                       <  1:6081788/93‑1 (MQ=255)
                                     gCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCtt                                                      <  2:6509047/93‑1 (MQ=255)
                                     gCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCtt                                                      <  2:259745/93‑1 (MQ=255)
                                     gCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCtt                                                      >  2:5715887/1‑93 (MQ=255)
                                      cGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                     <  1:1137244/93‑1 (MQ=255)
                                      cGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                     <  1:75729/93‑1 (MQ=255)
                                      cGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                     <  1:1846028/93‑1 (MQ=255)
                                       gACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTg                                                    <  1:4905949/93‑1 (MQ=255)
                                        aCTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCTCCGTGCTCCAGGTCTCCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                   <  1:3145172/93‑1 (MQ=255)
                                        aCTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                   <  2:4584066/93‑1 (MQ=255)
                                         cTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGAt                                                  >  1:2766792/1‑93 (MQ=255)
                                          tCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAGCTTCTTCGATATCTTTGATg                                                 >  1:5391006/1‑93 (MQ=255)
                                          tCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATg                                                 >  2:645148/1‑93 (MQ=255)
                                          tCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATg                                                 >  1:2014940/1‑93 (MQ=255)
                                            aCCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGcc                                               >  1:5924597/1‑93 (MQ=255)
                                             ccTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCg                                              >  1:6391121/1‑93 (MQ=255)
                                             ccTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCg                                              >  1:4917817/1‑93 (MQ=255)
                                              cTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGt                                             <  2:5898068/93‑1 (MQ=255)
                                                 cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTcc                                          >  2:3010487/1‑93 (MQ=255)
                                                  aaCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCca                                         >  2:6192894/1‑93 (MQ=255)
                                                   aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcac                                        >  2:2397062/1‑93 (MQ=255)
                                                   aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcac                                        >  2:1552424/1‑93 (MQ=255)
                                                    cGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcaca                                       <  2:4412776/93‑1 (MQ=255)
                                                     gTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAg                                      >  1:2094171/1‑93 (MQ=255)
                                                      tAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa                                     >  2:2551902/1‑93 (MQ=255)
                                                       aGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGag                                    >  1:2457756/1‑92 (MQ=255)
                                                        gTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACg                                   >  1:4338178/1‑93 (MQ=255)
                                                        gTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACg                                   >  2:1937522/1‑93 (MQ=255)
                                                         ttGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACgg                                  >  1:4159567/1‑93 (MQ=255)
                                                         ttGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACgg                                  <  1:2584916/93‑1 (MQ=255)
                                                          tGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGt                                 >  1:2159925/1‑93 (MQ=255)
                                                          tGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGt                                 >  1:473593/1‑93 (MQ=255)
                                                            aaGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTa                               <  1:4608596/93‑1 (MQ=255)
                                                            aaGCTGACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTa                               <  2:847105/93‑1 (MQ=255)
                                                              gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACg                             >  2:5429595/1‑93 (MQ=255)
                                                                 gACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                          <  2:324745/93‑1 (MQ=255)
                                                                 gACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                          <  2:3292758/93‑1 (MQ=255)
                                                                 gACGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                          >  2:747492/1‑93 (MQ=255)
                                                                 gACGTTGAGAATGGTCGGCGCACCGTGCTCCAGGTCACGGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                          <  1:1243963/93‑1 (MQ=255)
                                                                  aCGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAgg                         >  1:5709832/1‑93 (MQ=255)
                                                                  aCGTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAgg                         >  1:1592888/1‑93 (MQ=255)
                                                                    gTTGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGcc                       <  2:5597771/93‑1 (MQ=255)
                                                                      tGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCcgc                     <  1:5866943/93‑1 (MQ=255)
                                                                      tGAGAATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCcgc                     <  1:2950641/93‑1 (MQ=255)
                                                                       gagaATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCcgcg                    <  2:5673902/93‑1 (MQ=255)
                                                                        agaATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCgcgc                   >  1:1089538/1‑93 (MQ=255)
                                                                         gaATGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCa                  <  2:4261339/93‑1 (MQ=255)
                                                                          aaTGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAg                 >  1:3429118/1‑93 (MQ=255)
                                                                            tGTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGAc               >  2:5843446/1‑93 (MQ=255)
                                                                             gTTCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACg              >  1:834588/1‑93 (MQ=255)
                                                                               tCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGtt            >  2:988063/1‑93 (MQ=255)
                                                                               tCGGCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGtt            >  2:2080232/1‑93 (MQ=255)
                                                                                cGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTc           >  1:3815061/1‑93 (MQ=255)
                                                                                 ggCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTcc          <  1:3664148/93‑1 (MQ=255)
                                                                                 ggCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTcc          <  2:3470598/93‑1 (MQ=255)
                                                                                 ggCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTcc          <  1:3514275/93‑1 (MQ=255)
                                                                                 ggCGCACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTcc          >  2:5153993/1‑93 (MQ=255)
                                                                                    gcACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtc       <  2:1652225/93‑1 (MQ=255)
                                                                                    gcACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtc       >  2:5578284/1‑93 (MQ=255)
                                                                                     cACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtct      <  2:6292903/93‑1 (MQ=255)
                                                                                     cACCGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtct      <  1:5027861/93‑1 (MQ=255)
                                                                                      cccGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtctc     <  2:139359/92‑1 (MQ=255)
                                                                                       ccGTGCTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCg    >  1:1723899/1‑93 (MQ=255)
                                                                                         gtgcTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGt  <  2:4116035/93‑1 (MQ=255)
                                                                                            |                                                                                         
CCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGT  >  NC_000913/2658578‑2658759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑