BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,853	T→C	intergenic (‑186/+130)	serV ← / ← csrA	tRNA‑Ser/CsrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,853	0	T	C	90.8%	817.3 / 54.8	272	intergenic (‑186/+130)	serV/csrA	tRNA‑Ser/CsrA
Reads supporting (aligned to +/- strand): ref base T (14/11); new base C (129/118); total (143/129)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 273 total aligned reads displayed.

TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT  >  NC_000913/2816763‑2816943

ttttCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCt                                                                                          <  1:2950489/93‑1 (MQ=255)

ttttCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCt                                                                                          <  1:2907706/93‑1 (MQ=255)

   tacTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGAc                                                                                       >  1:6551847/3‑93 (MQ=255)

     cTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAg                                                                                     >  1:5488510/1‑93 (MQ=255)

      tACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGa                                                                                    >  2:1765924/1‑93 (MQ=255)

      tACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGa                                                                                    <  2:1979615/93‑1 (MQ=255)

       acacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAt                                                                                   >  2:4439528/1‑93 (MQ=255)

       acacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAt                                                                                   >  1:341666/1‑93 (MQ=255)

        cacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAta                                                                                  >  1:958406/1‑93 (MQ=255)

           ttttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAtagta                                                                               <  2:3318220/93‑1 (MQ=255)

            tttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGAtagtag                                                                              >  2:6212308/1‑93 (MQ=255)

            tttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAtagtag                                                                              >  1:6112059/1‑93 (MQ=255)

             ttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATagtagt                                                                             <  1:3170472/93‑1 (MQ=255)

             ttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATagtagt                                                                             <  2:2413899/93‑1 (MQ=255)

               aTCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTaa                                                                           >  2:1153324/1‑93 (MQ=255)

                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          <  1:5813148/93‑1 (MQ=255)

                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          >  1:1444485/1‑93 (MQ=255)

                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          >  2:5796654/1‑93 (MQ=255)

                 cGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATg                                                                         <  2:2078665/93‑1 (MQ=255)

                 cGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATg                                                                         <  2:6355499/93‑1 (MQ=255)

                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  2:4798572/1‑93 (MQ=255)

                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  2:6062856/1‑93 (MQ=255)

                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        <  1:4471640/93‑1 (MQ=255)

                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  1:4244287/1‑93 (MQ=255)

                   tttGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCa                                                                       >  1:4960893/1‑93 (MQ=255)

                   tttGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCa                                                                       >  1:2110814/1‑93 (MQ=255)

                      gCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCaaaa                                                                    >  1:3241934/1‑93 (MQ=255)

                       cacaCTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaa                                                                   >  2:1017164/1‑93 (MQ=255)

                        acacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaa                                                                  >  2:474705/1‑93 (MQ=255)

                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 <  1:2505290/93‑1 (MQ=255)

                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 >  2:2661917/1‑93 (MQ=255)

                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 <  2:2988911/93‑1 (MQ=255)

                           cTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATg                                                               >  2:5366053/1‑93 (MQ=255)

                             tCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGga                                                             >  2:2674128/1‑93 (MQ=255)

                             tCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGga                                                             <  2:5148525/93‑1 (MQ=255)

                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            >  2:6403406/1‑93 (MQ=255)

                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            <  1:1772002/93‑1 (MQ=255)

                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            <  2:5689327/93‑1 (MQ=255)

                               aCGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgaga                                                           >  2:6236671/1‑93 (MQ=255)

                               aCGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgaga                                                           <  1:523252/93‑1 (MQ=255)

                                cGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGAc                                                          <  2:4206048/93‑1 (MQ=255)

                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        >  2:4946629/1‑93 (MQ=255)

                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        <  2:3243648/93‑1 (MQ=255)

                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        >  2:1483332/1‑93 (MQ=255)

                                    cAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTaa                                                      <  2:36126/93‑1 (MQ=255)

                                      aTTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGt                                                    <  1:3372492/93‑1 (MQ=255)

                                      aTTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGt                                                    >  1:1863397/1‑93 (MQ=255)

                                       ttAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGtt                                                   <  1:4849780/93‑1 (MQ=255)

                                        tAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtg                                                  >  1:197148/1‑93 (MQ=255)

                                          gTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaa                                                <  2:4075670/93‑1 (MQ=255)

                                          gTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaa                                                >  1:675496/1‑93 (MQ=255)

                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  2:1304566/93‑1 (MQ=255)

                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               >  1:4562870/1‑93 (MQ=255)

                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  2:1662041/93‑1 (MQ=255)

                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  1:557999/93‑1 (MQ=255)

                                            cTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatg                                              <  2:2299773/93‑1 (MQ=255)

                                             tGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatga                                             <  1:6209365/93‑1 (MQ=255)

                                              gTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatgaa                                            >  1:1140469/1‑93 (MQ=255)

                                               tAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAAc                                           >  1:2738755/1‑93 (MQ=255)

                                                   caCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       <  2:5301700/92‑1 (MQ=255)

                                                   aaCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       <  2:4329306/93‑1 (MQ=255)

                                                   aaCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       >  2:5526609/1‑93 (MQ=255)

                                                    aCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAg                                      >  1:4263509/1‑93 (MQ=255)

                                                       tCAAAAAGAGTGTTTTATCGACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaa                                   <  2:6131005/93‑1 (MQ=255)

                                                       tCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaa                                   <  1:764692/93‑1 (MQ=255)

                                                        cAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaaa                                  <  1:1267769/93‑1 (MQ=255)

                                                         aaaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAg                                 <  2:4182980/93‑1 (MQ=255)

                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                >  2:3523521/1‑93 (MQ=255)

                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                >  2:3800605/1‑93 (MQ=255)

                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                <  1:2851552/93‑1 (MQ=255)

                                                           aaaGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCg                               >  2:3834362/1‑93 (MQ=255)

                                                             agagTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGaa                             >  2:786019/1‑93 (MQ=255)

                                                               agTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGaaaa                           <  2:2273247/93‑1 (MQ=255)

                                                                gtgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAg                          <  1:1606235/93‑1 (MQ=255)

                                                                 tgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGa                         >  2:2798837/1‑93 (MQ=255)

                                                                 tgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGa                         <  1:1592909/93‑1 (MQ=255)

                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        >  1:5066111/1‑93 (MQ=255)

                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        <  1:4191276/93‑1 (MQ=255)

                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        <  2:143251/93‑1 (MQ=255)

                                                                    tttATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      >  2:1205860/1‑93 (MQ=255)

                                                                    tttATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      <  1:2926062/93‑1 (MQ=255)

                                                                    tttATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      <  2:6615210/93‑1 (MQ=255)

                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    <  2:4377062/93‑1 (MQ=255)

                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    >  1:3839333/1‑93 (MQ=255)

                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    >  2:219061/1‑93 (MQ=255)

                                                                       aTCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATag                   >  2:5071627/1‑93 (MQ=255)

                                                                       aTCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATag                   <  2:6268412/93‑1 (MQ=255)

                                                                        tCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATaga                  <  1:3708237/93‑1 (MQ=255)

                                                                        tCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATaga                  <  1:3465921/93‑1 (MQ=255)

                                                                          aaCAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGt                >  2:5978790/1‑93 (MQ=255)

                                                                            cAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGa              >  2:4600954/1‑93 (MQ=255)

                                                                              gaagaaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAg            >  1:6313490/1‑93 (MQ=255)

                                                                               aagaaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAgg           <  1:5349824/93‑1 (MQ=255)

                                                                                  aaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGgaga        <  1:810758/93‑1 (MQ=255)

                                                                                   aTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaa       >  1:6037897/1‑93 (MQ=255)

                                                                                   aTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaa       >  1:6411049/1‑93 (MQ=255)

                                                                                    tGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaaa      >  2:255907/1‑93 (MQ=255)

                                                                                      gAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAtt    >  1:2283434/1‑93 (MQ=255)

                                                                                      gAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAtt    >  2:3867402/1‑93 (MQ=255)

                                                                                       aGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAttt   >  1:3974846/1‑93 (MQ=255)

TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT  >  NC_000913/2816763‑2816943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`