BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,857,315	C→T	Q734* (CAG→TAG)	mutS →	MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,857,315	0	C	T	100.0%	1245.8 / NA	322	Q734* (CAG→TAG)	mutS	MutS
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (180/142); total (180/142)

Only 100 of 327 total aligned reads displayed.

GGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG  >  NC_000913/2857225‑2857404

ggTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAg                                                                                         <  2:4864726/93‑1 (MQ=255)

  tCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGtt                                                                                       <  1:2625667/93‑1 (MQ=255)

    tGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAc                                                                                     >  1:5300673/1‑93 (MQ=255)

     gTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAcc                                                                                    <  1:5646977/93‑1 (MQ=255)

     gTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTAcc                                                                                    >  2:4961999/1‑93 (MQ=255)

      tCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCg                                                                                   >  1:6034850/1‑93 (MQ=255)

      tCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCg                                                                                   <  1:2077862/93‑1 (MQ=255)

        gCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGga                                                                                 >  1:1945306/1‑93 (MQ=255)

        gCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGga                                                                                 <  2:697234/93‑1 (MQ=255)

        gCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGga                                                                                 <  1:6626836/93‑1 (MQ=255)

           ggcgtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGaa                                                                              >  2:160624/1‑93 (MQ=255)

           ggcgtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGaa                                                                              >  1:3580052/1‑93 (MQ=255)

              gtgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAAt                                                                           >  2:2664629/1‑93 (MQ=255)

                gggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATgg                                                                         <  1:4590050/93‑1 (MQ=255)

                   cgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAg                                                                      <  2:412486/93‑1 (MQ=255)

                    gtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAgg                                                                     >  2:4170014/1‑93 (MQ=255)

                    gtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAgg                                                                     >  1:4500828/1‑93 (MQ=255)

                      gcgcGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCg                                                                   >  2:2779695/1‑93 (MQ=255)

                      gcgcGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCg                                                                   <  2:3134988/93‑1 (MQ=255)

                      gcgcGGAAAATCGGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCg                                                                   <  2:471403/93‑1 (MQ=255)

                       cgcgGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGt                                                                  <  2:4394927/93‑1 (MQ=255)

                        gcgGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTc                                                                 <  2:818758/93‑1 (MQ=255)

                           gAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTTGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCt                                                              >  1:5200224/1‑93 (MQ=255)

                            aaaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTa                                                             <  1:1286448/93‑1 (MQ=255)

                            aaaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTa                                                             <  2:3929171/93‑1 (MQ=255)

                            aaaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTa                                                             >  1:3882367/1‑93 (MQ=255)

                             aaaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTaa                                                            >  1:2258880/1‑93 (MQ=255)

                              aaTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAc                                                           <  2:3580651/93‑1 (MQ=255)

                               aTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACg                                                          >  1:5597990/1‑93 (MQ=255)

                                tCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGt                                                         <  2:2798106/93‑1 (MQ=255)

                                tCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGt                                                         >  2:5051103/1‑93 (MQ=255)

                                  tGGCGAATAAGATTAAGGCCTTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGc                                                       >  2:1143119/1‑93 (MQ=255)

                                  tGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGc                                                       <  1:2179429/93‑1 (MQ=255)

                                  tGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGc                                                       >  1:3233558/1‑93 (MQ=255)

                                   ggCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAAAGTGCa                                                      <  2:1702140/93‑1 (MQ=255)

                                    gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAt                                                     >  1:4333364/1‑93 (MQ=255)

                                    gCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAt                                                     <  2:5578347/93‑1 (MQ=255)

                                      gAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtct                                                   <  1:2770526/93‑1 (MQ=255)

                                      gAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtct                                                   >  2:1050595/1‑93 (MQ=255)

                                      gAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtct                                                   <  2:3783897/93‑1 (MQ=255)

                                       aaTAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtctc                                                  >  2:1206955/1‑93 (MQ=255)

                                       aaTAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCAtctc                                                  <  1:961830/93‑1 (MQ=255)

                                       aaTAAGATTAAGGCATTGACGTTACTTGCTACCCACTATTTCGAGCTGACCTAGTTACAGGAGAAAATGGAAGGCGTCGCTAACGTGCAtctc                                                  <  2:4603591/93‑1 (MQ=255)

                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                >  2:5863563/1‑93 (MQ=255)

                                         tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGa                                                <  2:6298647/93‑1 (MQ=255)

                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              >  2:5730196/1‑93 (MQ=255)

                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              >  2:1506070/1‑93 (MQ=255)

                                           aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATg                                              >  1:5527555/1‑93 (MQ=255)

                                            gATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGc                                             <  2:5778181/93‑1 (MQ=255)

                                            gATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGc                                             >  2:737156/1‑93 (MQ=255)

                                              ttAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCAc                                           >  2:2006440/1‑93 (MQ=255)

                                               tAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACt                                          >  2:5405844/1‑93 (MQ=255)

                                               tAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACt                                          <  1:3009758/93‑1 (MQ=255)

                                                  ggCATTGAGGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       >  1:2450663/1‑93 (MQ=255)

                                                  ggCATTGACGTTATTTGCTGCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  2:3656595/93‑1 (MQ=255)

                                                  ggCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  1:2928420/93‑1 (MQ=255)

                                                  ggCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       >  2:3199090/1‑93 (MQ=255)

                                                  ggCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  1:4247446/93‑1 (MQ=255)

                                                  ggCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                       <  1:6331611/93‑1 (MQ=255)

                                                    cATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGc                                     >  2:6221878/1‑93 (MQ=255)

                                                    cATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGc                                     >  1:4734124/1‑93 (MQ=255)

                                                    cATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGc                                     <  1:689915/93‑1 (MQ=255)

                                                     aTTGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCa                                    <  1:1172436/93‑1 (MQ=255)

                                                      ttGACGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCAc                                   <  1:2882983/93‑1 (MQ=255)

                                                         aCGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGc                                <  1:5967205/93‑1 (MQ=255)

                                                         aCGTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGc                                >  1:1749048/1‑93 (MQ=255)

                                                           gTTATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGa                              >  1:3380658/1‑93 (MQ=255)

                                                             tATTTGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGaca                            >  2:4803947/1‑93 (MQ=255)

                                                               tttGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACAcc                          >  1:4020032/1‑93 (MQ=255)

                                                                 tGCTACCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAt                        <  1:406137/93‑1 (MQ=255)

                                                                     aCCCACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGcc                    <  2:2602681/93‑1 (MQ=255)

                                                                       ccACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCtt                  >  1:6127618/1‑93 (MQ=255)

                                                                        cACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCCCGGCGACACGAGTGCCttt                 >  1:4006069/1‑93 (MQ=255)

                                                                        cACTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCttt                 >  1:889881/1‑93 (MQ=255)

                                                                         ccTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTa                <  1:3521732/92‑1 (MQ=255)

                                                                         aCTATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTa                >  1:159316/1‑93 (MQ=255)

                                                                           tATTTCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATg              >  2:2863397/1‑93 (MQ=255)

                                                                              ttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcac           >  1:169508/1‑93 (MQ=255)

                                                                              ttCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCGTTATGcac           <  1:6448275/93‑1 (MQ=255)

                                                                               tCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcaca          >  2:2552342/1‑93 (MQ=255)

                                                                               tCGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGcaca          >  2:1864332/1‑93 (MQ=255)

                                                                                cGAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAg         <  1:4788884/93‑1 (MQ=255)

                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        >  1:6156686/1‑93 (MQ=255)

                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        >  1:1864341/1‑93 (MQ=255)

                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        <  2:3861520/93‑1 (MQ=255)

                                                                                 gAGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGc        <  1:6240500/93‑1 (MQ=255)

                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       >  1:3630514/1‑93 (MQ=255)

                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       >  2:5344900/1‑93 (MQ=255)

                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       >  2:2315101/1‑93 (MQ=255)

                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCg       >  2:1431771/1‑93 (MQ=255)

                                                                                  aGCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACNGGNGCACGGCGACACCATTGCCTTTATGCACAGCg       >  1:5096361/1‑93 (MQ=255)

                                                                                   gCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGt      <  2:3493071/93‑1 (MQ=255)

                                                                                   gCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGt      >  2:3234988/1‑93 (MQ=255)

                                                                                   gCTGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGt      >  2:190655/1‑93 (MQ=255)

                                                                                   gCTGACCTAGTTACCGGAGAAAACGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGt      >  2:3435072/1‑93 (MQ=255)

                                                                                     tGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGc    >  1:1090283/1‑93 (MQ=255)

                                                                                     tGACCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATATCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGc    <  1:4943942/93‑1 (MQ=255)

                                                                                       aCCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAg  <  1:4999006/93‑1 (MQ=255)

                                                                                       aCCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAg  >  2:6483951/1‑93 (MQ=255)

                                                                                       aCCTAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAg  >  2:664371/1‑93 (MQ=255)

GGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAG  >  NC_000913/2857225‑2857404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`