BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,601	A→G	A121A (GCT→GCC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,601	0	A	G	84.5%	606.6 / 102.5	246	A121A (GCT→GCC)	eno	enolase
Reads supporting (aligned to +/- strand): ref base A (22/16); new base G (96/112); total (118/128)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 259 total aligned reads displayed.

CATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG  >  NC_000913/2905511‑2905691

 atcatcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCt                                                                                         >  2:5683135/1‑93 (MQ=255)

 atcatcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCt                                                                                         <  2:3489920/93‑1 (MQ=255)

   catcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTGCAGCGGCATACCTTTAGCAGCTGCAGCGGCttt                                                                                       >  2:6480931/1‑93 (MQ=255)

    atcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTg                                                                                      <  1:3135994/93‑1 (MQ=255)

         gaCCGGCATAGGGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt                                                                                 <  2:1524164/92‑1 (MQ=255)

          aCCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTa                                                                                >  2:3864328/1‑93 (MQ=255)

          aCCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTa                                                                                <  1:6229853/93‑1 (MQ=255)

           ccGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAg                                                                               >  1:6405198/1‑93 (MQ=255)

            cGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGc                                                                              >  2:3215663/1‑93 (MQ=255)

                aTAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCaga                                                                          <  2:5727623/93‑1 (MQ=255)

                aTAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCaga                                                                          <  1:1263884/93‑1 (MQ=255)

                 tAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCagag                                                                         >  2:786869/1‑93 (MQ=255)

                   gagTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGAt                                                                       >  1:2955342/1‑93 (MQ=255)

                        tttGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGc                                                                  <  2:663291/93‑1 (MQ=255)

                        tttGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCAGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGc                                                                  <  2:2817045/93‑1 (MQ=255)

                           gCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAg                                                               <  2:1389078/93‑1 (MQ=255)

                           gCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAg                                                               >  2:1725905/1‑93 (MQ=255)

                            cccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAgg                                                              >  2:3128798/1‑93 (MQ=255)

                             ccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGa                                                             >  2:6618638/1‑93 (MQ=255)

                             ccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGa                                                             <  1:4482847/93‑1 (MQ=255)

                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            <  1:2725487/93‑1 (MQ=255)

                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            <  1:1152292/93‑1 (MQ=255)

                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            >  2:4419743/1‑93 (MQ=255)

                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           >  2:3601075/1‑93 (MQ=255)

                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           <  1:4703911/93‑1 (MQ=255)

                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           >  1:1512453/1‑93 (MQ=255)

                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          >  1:1028875/1‑93 (MQ=255)

                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          <  2:5427072/93‑1 (MQ=255)

                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          >  2:5536771/1‑93 (MQ=255)

                                  gTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCg                                                        >  1:1352647/1‑93 (MQ=255)

                                   tACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGt                                                       <  2:685953/93‑1 (MQ=255)

                                    aCCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGtt                                                      <  1:3938521/93‑1 (MQ=255)

                                      cGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcg                                                    <  2:3985169/93‑1 (MQ=255)

                                      cGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcg                                                    <  2:5577797/93‑1 (MQ=255)

                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  1:3654852/1‑93 (MQ=255)

                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  2:6039921/1‑93 (MQ=255)

                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  2:693919/1‑93 (MQ=255)

                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:5209119/1‑93 (MQ=255)

                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:204082/1‑93 (MQ=255)

                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:2161059/1‑93 (MQ=255)

                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  <  2:3820319/93‑1 (MQ=255)

                                         tcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCgcgc                                                 >  1:2323170/1‑93 (MQ=255)

                                           agttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCg                                               >  2:5807016/1‑93 (MQ=255)

                                            gttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGa                                              >  1:2579922/1‑93 (MQ=255)

                                            gttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGa                                              >  2:1699281/1‑93 (MQ=255)

                                             ttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGaa                                             <  2:6166436/93‑1 (MQ=255)

                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTg                                         <  2:2695526/93‑1 (MQ=255)

                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTg                                         <  1:2384752/93‑1 (MQ=255)

                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGAGTTCGCGCCGAATTTg                                         >  2:559716/1‑93 (MQ=255)

                                                  cGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTgg                                        >  2:1887674/1‑93 (MQ=255)

                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      <  1:1678797/93‑1 (MQ=255)

                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      <  1:4093863/93‑1 (MQ=255)

                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGAGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      >  2:6554434/1‑93 (MQ=255)

                                                     tgtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTTGAtt                                     <  1:5902860/93‑1 (MQ=255)

                                                     tgtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAtt                                     >  2:2346535/1‑93 (MQ=255)

                                                      gtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAttt                                    >  1:4899176/1‑93 (MQ=255)

                                                       tgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTg                                   >  2:5981054/1‑93 (MQ=255)

                                                       tgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTg                                   >  1:1134671/1‑93 (MQ=255)

                                                        gCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGt                                  <  1:3501384/93‑1 (MQ=255)

                                                        gCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGt                                  <  1:1886347/93‑1 (MQ=255)

                                                         cTCGTACAGCGTCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  1:4301924/93‑1 (MQ=255)

                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  2:5263080/93‑1 (MQ=255)

                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 >  2:2673100/1‑93 (MQ=255)

                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 >  1:5366537/1‑93 (MQ=255)

                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  1:1604602/93‑1 (MQ=255)

                                                          tCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGttt                                >  1:6556187/1‑93 (MQ=255)

                                                          tCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGttt                                >  1:5729162/1‑93 (MQ=255)

                                                           cGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtttt                               <  1:3275046/93‑1 (MQ=255)

                                                            gTACAGCGTCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtttta                              >  1:6445428/1‑92 (MQ=255)

                                                            gTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTc                              >  1:1749466/1‑93 (MQ=255)

                                                             tACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCg                             <  1:2045368/93‑1 (MQ=255)

                                                             tACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCg                             <  1:891946/93‑1 (MQ=255)

                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           <  2:4879086/93‑1 (MQ=255)

                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           <  1:4372873/93‑1 (MQ=255)

                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           >  2:3853574/1‑93 (MQ=255)

                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           >  1:5549258/1‑93 (MQ=255)

                                                                aGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTg                          >  1:6151743/1‑93 (MQ=255)

                                                                aGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTg                          >  2:1428168/1‑93 (MQ=255)

                                                                 gCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGc                         <  1:5279216/93‑1 (MQ=255)

                                                                  cGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGcc                        >  2:4947097/1‑93 (MQ=255)

                                                                  cGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGcc                        <  1:3974411/93‑1 (MQ=255)

                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       <  2:3314513/93‑1 (MQ=255)

                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       >  2:1897029/1‑93 (MQ=255)

                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       <  1:955608/93‑1 (MQ=255)

                                                                    gCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGt                      >  2:3781888/1‑93 (MQ=255)

                                                                       tACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCa                   <  2:3892773/93‑1 (MQ=255)

                                                                        aCCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAg                  <  1:1304107/93‑1 (MQ=255)

                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                <  2:286418/93‑1 (MQ=255)

                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                >  1:2221100/1‑93 (MQ=255)

                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                <  1:3706840/93‑1 (MQ=255)

                                                                            ttAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCg              <  2:4108261/93‑1 (MQ=255)

                                                                             tAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             >  1:3302643/1‑93 (MQ=255)

                                                                             tAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             <  2:4292202/93‑1 (MQ=255)

                                                                             tAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             <  1:3031130/93‑1 (MQ=255)

                                                                              agcagcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGAt            >  2:5089603/1‑93 (MQ=255)

                                                                                cagcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCa          >  2:2665617/1‑93 (MQ=255)

                                                                                cagcTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCa          >  1:2931662/1‑93 (MQ=255)

                                                                                 agcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCAt         >  1:5570060/1‑93 (MQ=255)

                                                                                    tGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGAt      >  2:4598561/1‑93 (MQ=255)

                                                                                     gcagcgGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATc     <  1:6307990/93‑1 (MQ=255)

CATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG  >  NC_000913/2905511‑2905691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`