BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,285	T→C	D135G (GAC→GGC)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,285	0	T	C	85.8%	753.4 / 108.8	288	D135G (GAC→GGC)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base T (14/27); new base C (118/129); total (132/156)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

Only 100 of 292 total aligned reads displayed.

GCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGG  >  NC_000913/2907194‑2907377

  cAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATCCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCAt                                                                                           <  1:5617715/93‑1 (MQ=255)

   aaTTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCCAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATg                                                                                          >  1:1615607/1‑93 (MQ=255)

   aaTTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATg                                                                                          >  2:4958436/1‑93 (MQ=255)

    aTTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGa                                                                                         >  2:6405189/1‑93 (MQ=255)

       tCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCt                                                                                      <  1:4756134/93‑1 (MQ=255)

       tCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCt                                                                                      <  1:338505/93‑1 (MQ=255)

        cAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCtt                                                                                     >  2:6123178/1‑93 (MQ=255)

        cAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCtt                                                                                     <  2:3980411/93‑1 (MQ=255)

        cAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCtt                                                                                     <  2:4314287/93‑1 (MQ=255)

         aaCAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTc                                                                                    >  2:5634426/1‑93 (MQ=255)

           cAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGc                                                                                  <  2:4041590/93‑1 (MQ=255)

            aGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGcc                                                                                 <  1:4567779/93‑1 (MQ=255)

            aGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGcc                                                                                 <  2:3772312/93‑1 (MQ=255)

            aGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGcc                                                                                 >  2:4761507/1‑93 (MQ=255)

             gCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCa                                                                                >  2:5303624/1‑93 (MQ=255)

                aTCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCt                                                                             <  1:3780104/93‑1 (MQ=255)

                aTCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCt                                                                             >  2:837645/1‑93 (MQ=255)

                aTCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCt                                                                             <  1:1362345/93‑1 (MQ=255)

                    ggCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCa                                                                         >  1:3427417/1‑93 (MQ=255)

                      cGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGc                                                                       >  2:3380429/1‑93 (MQ=255)

                       gAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCa                                                                      >  1:5203200/1‑93 (MQ=255)

                       caaTCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCa                                                                      <  1:1128929/92‑1 (MQ=255)

                         aTCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCAcg                                                                    >  1:1408228/1‑93 (MQ=255)

                         aTCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGAACTTCGCGACCTTCCAGCAcg                                                                    >  1:832658/1‑93 (MQ=255)

                          acGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcgc                                                                   <  2:5452404/92‑1 (MQ=255)

                             cTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGct                                                                >  2:5562932/1‑93 (MQ=255)

                              ttCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGctc                                                               <  1:5793137/93‑1 (MQ=255)

                              ttCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGctc                                                               >  2:4178190/1‑93 (MQ=255)

                                 gAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCttt                                                            <  1:189105/93‑1 (MQ=255)

                                 gAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCttt                                                            <  1:3125785/93‑1 (MQ=255)

                                 gAGGAACGGCAAGGATTCGATATCACCTACTGTAACGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCttt                                                            <  1:4679024/93‑1 (MQ=255)

                                   ggAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGa                                                          <  1:5095097/93‑1 (MQ=255)

                                    gcaCGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGAt                                                         >  1:955003/3‑93 (MQ=255)

                                    gAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGAt                                                         <  1:6602603/93‑1 (MQ=255)

                                      aCGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTg                                                       >  2:945936/1‑93 (MQ=255)

                                      aCGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTg                                                       >  2:4699976/1‑93 (MQ=255)

                                      aCGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTg                                                       <  1:6306250/93‑1 (MQ=255)

                                      aCGGCAAGGATTCGATATCACCCACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTg                                                       >  1:1576932/1‑93 (MQ=255)

                                       cGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGc                                                      >  2:3762730/1‑93 (MQ=255)

                                       cGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGc                                                      >  1:4307209/1‑93 (MQ=255)

                                         gCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACACGTACTACGCCATGACCTTCCCGACCTTCCAGCACGCGCTCTTTGATTGCGt                                                    >  2:1443651/1‑93 (MQ=255)

                                          cAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGtt                                                   <  1:1649454/93‑1 (MQ=255)

                                           aaGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTa                                                  >  2:729742/1‑93 (MQ=255)

                                            aGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAg                                                 >  1:6349086/1‑93 (MQ=255)

                                             ggATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                                <  2:3476314/93‑1 (MQ=255)

                                             ggATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                                >  2:5988004/1‑93 (MQ=255)

                                              gATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTg                                               <  1:2962416/93‑1 (MQ=255)

                                              gATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTg                                               >  1:3616118/1‑93 (MQ=255)

                                                ttCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAt                                             >  1:1812216/1‑93 (MQ=255)

                                                 tCGATATCACCTACTGTCCCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCCCCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtg                                            <  1:1263054/93‑1 (MQ=255)

                                                 tCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtg                                            <  1:4379991/93‑1 (MQ=255)

                                                 tCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtg                                            <  1:5448307/93‑1 (MQ=255)

                                                   gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgtg                                          >  1:326237/1‑93 (MQ=255)

                                                     tatCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCg                                        <  2:134535/93‑1 (MQ=255)

                                                     tatCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCg                                        <  2:3346818/93‑1 (MQ=255)

                                                     tatCACCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGACACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCg                                        <  2:3560154/93‑1 (MQ=255)

                                                        cACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGaa                                     <  1:2389711/93‑1 (MQ=255)

                                                         aCCTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAAt                                    <  2:3029494/93‑1 (MQ=255)

                                                          ccTACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATa                                   <  2:1532134/93‑1 (MQ=255)

                                                            tACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAc                                 >  1:4214902/1‑93 (MQ=255)

                                                            tACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAc                                 >  2:2738275/1‑93 (MQ=255)

                                                            tACTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAc                                 >  2:1966148/1‑93 (MQ=255)

                                                             tcTGTACCGCCGATTTCTACCAGTACTACGCCATGACCGTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAcc                                <  1:3058143/92‑1 (MQ=255)

                                                             aCTGTGCCGCCGATCTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAcc                                <  2:1053817/93‑1 (MQ=255)

                                                              cTGTACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCt                               <  1:596920/93‑1 (MQ=255)

                                                                 tACCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCa                            <  1:4850336/93‑1 (MQ=255)

                                                                  aCCGCCGATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCAc                           <  1:2294237/93‑1 (MQ=255)

                                                                    cgccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACgg                         >  1:4182071/1‑93 (MQ=255)

                                                                    cgccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACgg                         <  1:3868941/93‑1 (MQ=255)

                                                                     gccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGt                        >  1:2527761/1‑93 (MQ=255)

                                                                     gccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGt                        >  2:5135011/1‑93 (MQ=255)

                                                                     gccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGt                        <  1:5711474/93‑1 (MQ=255)

                                                                     gccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGt                        <  1:3500935/93‑1 (MQ=255)

                                                                      ccgATTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGtt                       <  2:1806547/93‑1 (MQ=255)

                                                                       cgATTTCTACCAGTACTACGTCATGACCTTCGCAACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTg                      >  2:6481846/1‑93 (MQ=255)

                                                                        caTTTCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTgc                     <  2:2154415/92‑1 (MQ=255)

                                                                          tttCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTgcgc                   >  2:2591185/1‑93 (MQ=255)

                                                                          tttCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTgcgc                   >  1:3929845/1‑93 (MQ=255)

                                                                           ttCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGcc                  <  2:5735691/93‑1 (MQ=255)

                                                                            tCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCg                 <  2:2374479/93‑1 (MQ=255)

                                                                            tCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCg                 >  1:4468725/1‑93 (MQ=255)

                                                                            tCTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCg                 <  1:2183165/93‑1 (MQ=255)

                                                                             cTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGa                >  1:441727/1‑93 (MQ=255)

                                                                             cTACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGa                <  2:4103929/93‑1 (MQ=255)

                                                                              tACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAg               >  2:2295741/1‑93 (MQ=255)

                                                                              tACCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAg               >  1:5025848/1‑93 (MQ=255)

                                                                               aCCAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAgg              >  2:5848847/1‑93 (MQ=255)

                                                                                ccAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGt             <  1:2634099/93‑1 (MQ=255)

                                                                                ccAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGt             <  2:1991332/93‑1 (MQ=255)

                                                                                 cAGTACTGCGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTa            <  1:728192/93‑1 (MQ=255)

                                                                                 cAGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTa            >  2:2546126/1‑93 (MQ=255)

                                                                                  aGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTTCGCCGAGGTAg           >  1:4008358/1‑93 (MQ=255)

                                                                                  aGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAg           >  1:534115/1‑93 (MQ=255)

                                                                                  aGTACTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAg           <  1:495899/93‑1 (MQ=255)

                                                                                   gTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGt          >  2:1703725/1‑93 (MQ=255)

                                                                                    tactacGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTc         >  1:578022/1‑93 (MQ=255)

                                                                                       tacGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCAcc      <  1:2003097/93‑1 (MQ=255)

                                                                                       tacGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCAcc      <  2:3091315/93‑1 (MQ=255)

                                                                                        acGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACcg     <  1:102595/93‑1 (MQ=255)

                                                                                           tCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCgcgg  <  1:6108272/93‑1 (MQ=255)

GCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGG  >  NC_000913/2907194‑2907377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`