BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,753	T→C	A568A (GCA→GCG)	ptsP ←	PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,753	0	T	C	85.8%	685.4 / 96.0	267	A568A (GCA→GCG)	ptsP	PtsP
Reads supporting (aligned to +/- strand): ref base T (24/14); new base C (125/104); total (149/118)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 276 total aligned reads displayed.

CACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCA  >  NC_000913/2964663‑2964841

cACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGc                                                                                        >  2:4471085/1‑93 (MQ=255)

   ttCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTtc                                                                                     <  1:4385530/93‑1 (MQ=255)

    tCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTtca                                                                                    >  2:3066523/1‑93 (MQ=255)

       aGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTtcatca                                                                                 >  1:5352120/1‑93 (MQ=255)

       aGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACATTCAATCAGGCGGCGCGCTtcatca                                                                                 >  2:6420170/1‑93 (MQ=255)

        gCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCaa                                                                                >  2:146756/1‑93 (MQ=255)

        gCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCAGCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCaa                                                                                >  2:4662276/1‑93 (MQ=255)

         cATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAAc                                                                               >  1:1728466/1‑93 (MQ=255)

         cATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAAc                                                                               <  2:3536257/93‑1 (MQ=255)

          atgatgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACt                                                                              >  1:3651200/1‑93 (MQ=255)

            gatgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTc                                                                            <  1:2324462/93‑1 (MQ=255)

             atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCa                                                                           <  2:4817722/93‑1 (MQ=255)

             atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCa                                                                           >  1:5367153/1‑93 (MQ=255)

              tgCCGATACGTGGTTTGGGAATTTCGTACCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCAt                                                                          <  1:5807506/93‑1 (MQ=255)

              tgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCAt                                                                          <  1:791665/93‑1 (MQ=255)

               gCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATc                                                                         >  2:1284649/1‑93 (MQ=255)

               gCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATc                                                                         <  1:2828202/93‑1 (MQ=255)

                ccGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCg                                                                        <  1:4785003/93‑1 (MQ=255)

                ccGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCg                                                                        >  2:2544128/1‑93 (MQ=255)

                 cGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGa                                                                       >  1:1735332/1‑93 (MQ=255)

                 cGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGa                                                                       <  1:3256418/93‑1 (MQ=255)

                  gATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAg                                                                      <  1:5924573/93‑1 (MQ=255)

                   aTACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAgg                                                                     >  1:3532943/1‑93 (MQ=255)

                     aCGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCTCTTCATCAACTTCATCGAGGCt                                                                   <  1:4079790/93‑1 (MQ=255)

                      cGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCtt                                                                  <  2:5914900/93‑1 (MQ=255)

                        tGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTtgt                                                                >  2:3700562/1‑93 (MQ=255)

                        tGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTtgt                                                                <  1:3285983/93‑1 (MQ=255)

                          gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGa                                                              >  1:4012519/1‑93 (MQ=255)

                          gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGa                                                              >  1:5764580/1‑93 (MQ=255)

                             tGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCa                                                           <  2:6260248/93‑1 (MQ=255)

                              gggAATTTCGTAACCGATCATCTCCTCGACTTCACGGCCGGCACGTTCAATCAGTGGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCAt                                                          >  2:4298519/1‑93 (MQ=255)

                               ggAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATc                                                         <  1:1628676/93‑1 (MQ=255)

                                gAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCg                                                        >  2:2896281/1‑93 (MQ=255)

                                gAATTTCGTAACCGATCAGCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCg                                                        <  1:1297268/93‑1 (MQ=255)

                                 aaTTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCgg                                                       >  1:4616532/1‑93 (MQ=255)

                                  aTTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGc                                                      <  2:5192598/93‑1 (MQ=255)

                                  aTTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGc                                                      >  2:2297317/1‑93 (MQ=255)

                                   tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                                     <  1:2639624/93‑1 (MQ=255)

                                     tCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAAc                                                   <  1:1170710/93‑1 (MQ=255)

                                     tCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAAc                                                   <  1:1430493/93‑1 (MQ=255)

                                       gTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                 <  2:204347/93‑1 (MQ=255)

                                        tAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTTAACATCAGCCACAGa                                                >  1:2508836/1‑93 (MQ=255)

                                        tAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGa                                                >  2:942747/1‑93 (MQ=255)

                                        tAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGa                                                >  1:6368310/1‑93 (MQ=255)

                                        tAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGa                                                <  2:6103291/93‑1 (MQ=255)

                                        tAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGa                                                <  2:5499849/93‑1 (MQ=255)

                                          aCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAat                                              >  1:311866/1‑93 (MQ=255)

                                           ccGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAata                                             <  2:5881549/93‑1 (MQ=255)

                                             gATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATAtt                                           <  2:4548687/93‑1 (MQ=255)

                                             gATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATAtt                                           <  2:1706970/93‑1 (MQ=255)

                                               tcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCa                                         <  1:5256894/93‑1 (MQ=255)

                                               tcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCa                                         <  2:5749419/93‑1 (MQ=255)

                                                catcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAg                                        <  1:5011048/93‑1 (MQ=255)

                                                 atcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAgg                                       <  2:4041728/93‑1 (MQ=255)

                                                 atcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAgg                                       >  2:3280585/1‑93 (MQ=255)

                                                  tctcCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGt                                      >  1:3187101/1‑93 (MQ=255)

                                                  tctcCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGt                                      <  1:6453103/93‑1 (MQ=255)

                                                  tctcCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGt                                      >  1:62324/1‑93 (MQ=255)

                                                    tcctcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTg                                    >  2:3962684/1‑93 (MQ=255)

                                                    tcctcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTg                                    >  1:632214/1‑93 (MQ=255)

                                                     cctcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGc                                   >  1:1021868/1‑93 (MQ=255)

                                                      ctcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGcc                                  >  2:2260530/1‑93 (MQ=255)

                                                      ctcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGcc                                  >  2:3836698/1‑93 (MQ=255)

                                                       tcGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGccc                                 >  1:6598613/1‑93 (MQ=255)

                                                        cGACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCg                                <  2:3266069/93‑1 (MQ=255)

                                                         gACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGt                               >  2:1183232/1‑93 (MQ=255)

                                                         gACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGt                               <  1:5425490/93‑1 (MQ=255)

                                                         gACTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGt                               >  1:1207129/1‑93 (MQ=255)

                                                          gcTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                              <  1:2261206/92‑1 (MQ=255)

                                                          aCTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                              <  1:505473/93‑1 (MQ=255)

                                                          aCTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                              >  2:3634957/1‑93 (MQ=255)

                                                          aCTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                              <  1:4292858/93‑1 (MQ=255)

                                                          aCTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                              >  2:3753545/1‑93 (MQ=255)

                                                           cTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGGTGCCCGTAg                             >  2:1864031/1‑93 (MQ=255)

                                                           cTTCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAg                             >  1:465767/1‑93 (MQ=255)

                                                            ttCACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc                            >  2:3114166/1‑93 (MQ=255)

                                                              cACGTCCGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCgg                          <  2:4391185/93‑1 (MQ=255)

                                                                  tCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAtt                      >  2:1794275/1‑93 (MQ=255)

                                                                    cGGCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAg                    >  2:5081217/1‑93 (MQ=255)

                                                                     ggCACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGc                   >  1:2145664/1‑93 (MQ=255)

                                                                       cACGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCAc                 <  1:4902098/93‑1 (MQ=255)

                                                                        aCGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACg                <  2:2937740/93‑1 (MQ=255)

                                                                         cGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgc               >  1:2727838/1‑93 (MQ=255)

                                                                         cGTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgc               <  1:2681970/93‑1 (MQ=255)

                                                                          gTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcg              >  1:142150/1‑92 (MQ=255)

                                                                          gTTCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgca              <  2:4254370/93‑1 (MQ=255)

                                                                            tCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagc            <  1:671968/93‑1 (MQ=255)

                                                                            tCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagc            <  2:6031464/93‑1 (MQ=255)

                                                                            tCAATCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagc            >  2:2382409/1‑93 (MQ=255)

                                                                              aaTCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt          <  2:2105424/93‑1 (MQ=255)

                                                                              aaTCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt          >  2:5311679/1‑93 (MQ=255)

                                                                              aaTCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt          <  1:1868191/93‑1 (MQ=255)

                                                                              aaTCAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt          >  2:2228216/1‑93 (MQ=255)

                                                                                 cAGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGc       <  1:6058710/93‑1 (MQ=255)

                                                                                  aGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc      <  2:1988373/93‑1 (MQ=255)

                                                                                  aGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc      <  2:6621288/93‑1 (MQ=255)

                                                                                  aGGCGGCGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc      >  1:5533503/1‑93 (MQ=255)

                                                                                   ggcggcGCTCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGccc     <  2:1026350/93‑1 (MQ=255)

                                                                                   ggcggcGCGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGccc     >  2:4228824/1‑93 (MQ=255)

                                                                                    gcgacgCGCTTCATCAACTTCATCGAGGCTGGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCg    <  1:1564918/93‑1 (MQ=255)

CACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCA  >  NC_000913/2964663‑2964841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`