BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,924	A→G	L294L (TTA→TTG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,924	0	A	G	100.0%	864.7 / NA	241	L294L (TTA→TTG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/125); total (116/125)

Only 100 of 247 total aligned reads displayed.

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

 gTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCg                                                                                        >  2:2332771/1‑93 (MQ=255)

  ttCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGCTGCCGa                                                                                       >  2:5072564/1‑93 (MQ=255)

   tCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaa                                                                                      >  2:2201227/1‑93 (MQ=255)

   tCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaa                                                                                      <  2:2425182/93‑1 (MQ=255)

    tcGTTCACCGTCAGCCTGATCCTCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  2:3009497/92‑1 (MQ=255)

     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    >  2:1081583/1‑93 (MQ=255)

      gTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACt                                                                                   <  1:846414/93‑1 (MQ=255)

       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  >  2:2583897/1‑93 (MQ=255)

       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  <  2:3651215/93‑1 (MQ=255)

       tgcACCGTCAGCCTGATCCGCCCCCTGCCCCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  <  1:2156798/91‑1 (MQ=255)

        tCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTgg                                                                                 <  2:4461666/93‑1 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                >  1:6240241/1‑93 (MQ=255)

          aCCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTc                                                                               <  2:754883/93‑1 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  1:285806/93‑1 (MQ=255)

              tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACtt                                                                           <  2:451746/93‑1 (MQ=255)

               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  2:3780090/93‑1 (MQ=255)

               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  1:3286876/93‑1 (MQ=255)

                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       <  2:6392046/93‑1 (MQ=255)

                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      <  1:5554553/93‑1 (MQ=255)

                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      >  2:3409105/1‑93 (MQ=255)

                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  1:835226/1‑93 (MQ=255)

                    tGATCCGCCCCCTACACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:3794248/1‑93 (MQ=255)

                     gATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGa                                                                    <  1:6361732/93‑1 (MQ=255)

                      aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAc                                                                   >  2:1181197/1‑93 (MQ=255)

                      aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAc                                                                   >  1:3615802/1‑93 (MQ=255)

                       tCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACg                                                                  <  1:2069928/93‑1 (MQ=255)

                       tCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACg                                                                  <  2:2807344/93‑1 (MQ=255)

                          gCCCCCTGCACCTCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTa                                                               <  1:116055/93‑1 (MQ=255)

                          gCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTa                                                               >  2:1452317/1‑93 (MQ=255)

                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAt                                                              >  2:4988410/1‑93 (MQ=255)

                            ccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtt                                                             >  1:6065633/1‑93 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  2:4591113/93‑1 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  1:56837/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  2:5653158/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           >  1:6420039/1‑93 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  1:4681055/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           >  2:4926510/1‑93 (MQ=255)

                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGTGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          >  1:1082421/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        >  1:2438829/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        >  1:630556/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        <  1:2696365/93‑1 (MQ=255)

                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       >  1:5120308/1‑93 (MQ=255)

                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       <  1:4310165/93‑1 (MQ=255)

                                    ccGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcgtc                                                     >  1:1251056/1‑93 (MQ=255)

                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    >  2:1512559/1‑93 (MQ=255)

                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    >  1:3237161/1‑93 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               >  2:3376774/1‑93 (MQ=255)

                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACg                                              >  2:1968633/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  1:169289/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  2:6123426/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  1:2198412/1‑93 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:2510534/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:3082962/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  1:1061440/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:6195296/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:4260252/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:2107414/1‑93 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        <  1:2542516/93‑1 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  2:6295564/1‑93 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  1:5414658/1‑93 (MQ=255)

                                                 gcgcTGATTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        <  1:4046377/93‑1 (MQ=255)

                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       <  1:188829/93‑1 (MQ=255)

                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       <  1:738699/93‑1 (MQ=255)

                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     >  1:5782382/1‑93 (MQ=255)

                                                      ggTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaaa                                   <  2:3984771/93‑1 (MQ=255)

                                                       gTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaacg                                  <  1:4187055/93‑3 (MQ=255)

                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 >  2:2827357/1‑93 (MQ=255)

                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 <  2:710306/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  1:4574587/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  2:4314308/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  1:5016566/93‑1 (MQ=255)

                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  2:5155557/93‑1 (MQ=255)

                                                             gCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAg                            <  2:6026686/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:3939695/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  2:3612283/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:4821257/93‑1 (MQ=255)

                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  1:2298952/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:6219531/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:6620650/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        >  2:1619257/1‑93 (MQ=255)

                                                                  tAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATcc                       <  2:5506059/93‑1 (MQ=255)

                                                                   aGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCt                      <  1:4619513/93‑1 (MQ=255)

                                                                   aGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCt                      <  1:913306/93‑1 (MQ=255)

                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    <  2:4045922/93‑1 (MQ=255)

                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    >  1:3489540/1‑93 (MQ=255)

                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 <  1:5230287/93‑1 (MQ=255)

                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 <  2:3311730/93‑1 (MQ=255)

                                                                          aTTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGAGACAGCATCCTCGGCATg               >  1:2255619/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  1:166055/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  1:1277153/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  2:6474804/1‑93 (MQ=255)

                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  2:1015850/93‑1 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  2:4661439/1‑93 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:2802450/1‑93 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:5035994/1‑93 (MQ=255)

                                                                                aaGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgc         >  1:6553330/1‑93 (MQ=255)

                                                                                 aGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgcc        >  2:5121011/1‑93 (MQ=255)

                                                                                   cGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccgc      >  1:1748365/1‑93 (MQ=255)

                                                                                     gggTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCgg    >  1:15283/1‑93 (MQ=255)

                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  >  2:3206364/1‑93 (MQ=255)

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`