BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,185,807	T→C	F131S (TTT→TCT)	yqiG →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,185,807	0	T	C	100.0%	1046.8 / NA	276	F131S (TTT→TCT)	yqiG	putative membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (148/128); total (148/128)

Only 100 of 277 total aligned reads displayed.

AGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACT  >  NC_000913/3185717‑3185896

aGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTg                                                                                         >  2:309840/1‑93 (MQ=255)

 gCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGc                                                                                        <  1:3392768/93‑1 (MQ=255)

 gCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGc                                                                                        <  1:1821179/93‑1 (MQ=255)

   aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                                                      >  1:3757499/1‑93 (MQ=255)

   aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                                                      >  1:5445722/1‑93 (MQ=255)

     aaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCt                                                                                    <  2:2118405/93‑1 (MQ=255)

        cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTTCACCTCAg                                                                                 >  2:5725067/1‑93 (MQ=255)

         tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                                                <  1:286086/93‑1 (MQ=255)

          gATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGc                                                                               >  1:3593756/1‑93 (MQ=255)

             cGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATg                                                                            <  2:1537880/93‑1 (MQ=255)

             cGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATg                                                                            <  2:581122/93‑1 (MQ=255)

              gATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATgg                                                                           <  2:4888242/93‑1 (MQ=255)

                  gttgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaa                                                                       >  2:1579095/1‑93 (MQ=255)

                  gttgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaa                                                                       >  2:5924390/1‑93 (MQ=255)

                     gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                                                    >  1:522807/1‑93 (MQ=255)

                      ttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATa                                                                   >  2:3354178/1‑93 (MQ=255)

                      ttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATa                                                                   <  1:723598/93‑1 (MQ=255)

                       tACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAc                                                                  <  2:849122/93‑1 (MQ=255)

                        aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                                                 >  2:689846/1‑93 (MQ=255)

                          ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                                               <  1:914627/93‑1 (MQ=255)

                          ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                                               >  1:307211/1‑93 (MQ=255)

                           cTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAg                                                              >  1:6312768/1‑93 (MQ=255)

                            tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                                                             >  1:2886046/1‑93 (MQ=255)

                            tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                                                             <  1:5192222/93‑1 (MQ=255)

                             aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                                                            <  1:5989839/93‑1 (MQ=255)

                             aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                                                            <  2:1959633/93‑1 (MQ=255)

                              tCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATg                                                           <  1:4127307/93‑1 (MQ=255)

                               cGAAAAAGAAAAACAAATTACACCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                                                          <  1:4573315/93‑1 (MQ=255)

                               cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                                                          >  2:4484489/1‑93 (MQ=255)

                                gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                                                         >  1:322860/1‑93 (MQ=255)

                                gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                                                         >  2:6304094/1‑93 (MQ=255)

                                gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                                                         >  2:386319/1‑93 (MQ=255)

                                  aaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCaaa                                                       <  2:523667/93‑1 (MQ=255)

                                  aaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCaaa                                                       >  1:5088523/1‑93 (MQ=255)

                                   aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                                                      <  2:4268028/93‑1 (MQ=255)

                                   aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                                                      >  1:3428545/1‑93 (MQ=255)

                                   aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                                                      <  1:6089995/93‑1 (MQ=255)

                                   aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                                                      <  1:2125043/93‑1 (MQ=255)

                                    aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                                                     >  1:1730028/1‑93 (MQ=255)

                                    aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                                                     >  2:1222297/1‑93 (MQ=255)

                                    aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                                                     >  2:551116/1‑93 (MQ=255)

                                      gaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTgg                                                   >  2:4582569/1‑93 (MQ=255)

                                      gaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTgg                                                   >  2:5556304/1‑93 (MQ=255)

                                       aaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGa                                                  <  1:409081/93‑1 (MQ=255)

                                       aaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGa                                                  <  2:1471178/93‑1 (MQ=255)

                                        aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                                                 >  1:3036256/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                >  1:2212967/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                >  1:3888082/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                >  2:4556009/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                >  1:2625940/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                >  2:3678179/1‑93 (MQ=255)

                                         aaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACg                                                <  1:2266178/93‑1 (MQ=255)

                                          aaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGc                                               >  2:2478661/1‑93 (MQ=255)

                                          aaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGc                                               >  1:5309790/1‑93 (MQ=255)

                                          aaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGc                                               >  1:4381987/1‑93 (MQ=255)

                                           aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc                                              >  1:4782761/1‑93 (MQ=255)

                                           aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc                                              <  1:605757/93‑1 (MQ=255)

                                              aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc                                           <  2:273468/93‑1 (MQ=255)

                                               aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct                                          >  1:1697466/1‑93 (MQ=255)

                                               aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct                                          <  2:1279996/93‑1 (MQ=255)

                                                  aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa                                       <  2:6127263/93‑1 (MQ=255)

                                                    aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt                                     <  2:3793774/93‑1 (MQ=255)

                                                    aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt                                     >  2:5441988/1‑93 (MQ=255)

                                                    aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt                                     >  1:1990645/1‑93 (MQ=255)

                                                     aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt                                    <  1:79143/93‑1 (MQ=255)

                                                     aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt                                    >  2:219769/1‑93 (MQ=255)

                                                     aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt                                    >  1:4315552/1‑93 (MQ=255)

                                                     aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt                                    >  2:3317771/1‑93 (MQ=255)

                                                       ttATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTg                                  <  1:1036985/93‑1 (MQ=255)

                                                       ttATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTg                                  >  1:4871896/1‑93 (MQ=255)

                                                        tATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTgg                                 >  2:3282125/1‑93 (MQ=255)

                                                          tctCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGaa                               >  2:4934202/3‑93 (MQ=255)

                                                            tCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATc                             >  1:2580134/1‑93 (MQ=255)

                                                            tCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATc                             <  2:5849054/93‑1 (MQ=255)

                                                             cGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATGCAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCa                            <  1:4751652/93‑1 (MQ=255)

                                                              gATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCAt                           <  2:3736393/93‑1 (MQ=255)

                                                              gATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCAt                           >  1:1355714/1‑93 (MQ=255)

                                                               aTAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATg                          <  1:5709254/93‑1 (MQ=255)

                                                                tAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATgg                         <  2:3621043/93‑1 (MQ=255)

                                                                tAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATgg                         >  2:2306597/1‑93 (MQ=255)

                                                                tAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATgg                         <  2:4029188/93‑1 (MQ=255)

                                                                tAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATgg                         >  1:641829/1‑93 (MQ=255)

                                                                  aaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTa                       >  2:2836451/1‑93 (MQ=255)

                                                                  aaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTa                       <  2:6507939/93‑1 (MQ=255)

                                                                    gggAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTAtt                     <  1:1136243/93‑1 (MQ=255)

                                                                    gggAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTAtt                     >  1:216544/1‑93 (MQ=255)

                                                                     ggAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTg                    >  1:4003882/1‑93 (MQ=255)

                                                                     ggAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTg                    >  1:2333266/1‑93 (MQ=255)

                                                                      gAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGc                   <  1:6458522/93‑1 (MQ=255)

                                                                      gAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGc                   >  1:4256358/1‑93 (MQ=255)

                                                                       aaaaTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCt                  >  2:6010332/1‑93 (MQ=255)

                                                                          aTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTggg               >  2:1800493/1‑93 (MQ=255)

                                                                             cAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCa            <  1:6216305/93‑1 (MQ=255)

                                                                             cAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCa            >  1:2705078/1‑93 (MQ=255)

                                                                              acttATCCATATCTGCACCTCAGGCATGGTTAAACTACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAt           >  1:765572/3‑93 (MQ=255)

                                                                              aaTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAt           >  2:4825678/1‑93 (MQ=255)

                                                                               aTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAtt          <  1:164224/93‑1 (MQ=255)

                                                                               aTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACACCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAtt          >  2:2631025/1‑93 (MQ=255)

                                                                                ttATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAttt         >  2:6033675/1‑93 (MQ=255)

                                                                                ttATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAttt         <  2:6545901/93‑1 (MQ=255)

AGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACT  >  NC_000913/3185717‑3185896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`