BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,197,191	T→C	Q31R (CAG→CGG)	glnE ←	GlnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,197,191	0	T	C	100.0%	1022.0 / NA	278	Q31R (CAG→CGG)	glnE	GlnE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (152/126); total (152/126)

Only 100 of 298 total aligned reads displayed.

GGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTC  >  NC_000913/3197101‑3197280

ggcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgt                                                                                         <  2:440565/93‑1 (MQ=255)

ggcggTTGGCTTTCCAGTCCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgt                                                                                         <  1:4211550/93‑1 (MQ=255)

 gcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        >  2:2956925/1‑93 (MQ=255)

 gcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        <  2:3597736/93‑1 (MQ=255)

 gcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        <  2:610309/93‑1 (MQ=255)

 gcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        >  1:6036471/1‑93 (MQ=255)

 gcggTTGACTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        <  2:2581918/93‑1 (MQ=255)

  cggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgc                                                                                       <  2:73444/93‑1 (MQ=255)

   ggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgcg                                                                                      >  2:3236702/1‑93 (MQ=255)

    gTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgcgc                                                                                     >  1:2360006/1‑93 (MQ=255)

     ttGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCt                                                                                    <  2:4164266/93‑1 (MQ=255)

       gtcTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTaa                                                                                  <  2:835574/91‑1 (MQ=255)

        gCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAg                                                                                 <  2:2260119/93‑1 (MQ=255)

         cTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGt                                                                                <  2:4107624/93‑1 (MQ=255)

          tttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTg                                                                               <  2:3743847/93‑1 (MQ=255)

           ttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              >  1:4909989/1‑93 (MQ=255)

           ttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              <  1:3733060/93‑1 (MQ=255)

           tcccATTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGAAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              <  1:6190674/91‑1 (MQ=255)

            tCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAt                                                                             <  1:313818/93‑1 (MQ=255)

            tCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAt                                                                             >  1:5867706/1‑93 (MQ=255)

             ccAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAtt                                                                            <  1:4042839/93‑1 (MQ=255)

              cAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTc                                                                           >  2:4427852/1‑93 (MQ=255)

              cAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTc                                                                           >  1:218855/1‑93 (MQ=255)

               aGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTcc                                                                          >  2:4886767/1‑93 (MQ=255)

               aGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTcc                                                                          >  2:6472424/1‑93 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         <  1:859001/93‑1 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         >  2:6403366/1‑93 (MQ=255)

                gTTCCGTCAGCCACTCTCGATGCGCCATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCTTGCGCTAAGTGATTcat                                                                         >  1:5338953/1‑91 (MQ=255)

                gTTCCGCCAGCCACTCTGGATGCGCAATCACGCTGTCCTTCACAAAATCACTAAAAGTAAGTACTGACTTTGACCGTGCGCTAAGTGATTCCt                                                                         >  1:1043954/1‑93 (MQ=255)

                  tCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCg                                                                       <  2:2167378/93‑1 (MQ=255)

                   ccGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCgg                                                                      <  1:1427337/93‑1 (MQ=255)

                    cGTCAGCCACGCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGc                                                                     <  1:5963807/93‑1 (MQ=255)

                     gTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCt                                                                    >  1:1707346/1‑93 (MQ=255)

                     gTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCt                                                                    >  1:5424675/1‑93 (MQ=255)

                      tCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTa                                                                   >  1:5486888/1‑93 (MQ=255)

                        aGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTaaa                                                                 >  1:2468596/1‑93 (MQ=255)

                         gCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAg                                                                <  1:1553843/93‑1 (MQ=255)

                          ccACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAgg                                                               <  2:200057/93‑1 (MQ=255)

                           cACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGc                                                              <  2:1590853/93‑1 (MQ=255)

                            aCTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGct                                                             >  1:1192092/1‑93 (MQ=255)

                            aCTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGct                                                             >  1:6589975/1‑93 (MQ=255)

                             ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctc                                                            >  2:3260142/1‑93 (MQ=255)

                             ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctc                                                            <  2:4546288/93‑1 (MQ=255)

                             atctGGATGCGCAATCACGCTGTACTGCACAAAATCACTAAAAGTAAGTACTGACTTGGCCCGTGCGCTAAGTGATGCCTCGGCTAAAGGctc                                                            <  1:3553158/92‑1 (MQ=255)

                              tctGGATGCGCACTCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctct                                                           <  1:3762314/93‑1 (MQ=255)

                              tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctct                                                           >  2:2490226/1‑93 (MQ=255)

                              tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctct                                                           <  1:5088988/93‑1 (MQ=255)

                              tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctct                                                           <  2:6286275/93‑1 (MQ=255)

                                tGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTgg                                                         <  2:101564/93‑1 (MQ=255)

                                tGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTgg                                                         <  2:1908843/93‑1 (MQ=255)

                                tGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTgg                                                         >  1:4794756/1‑93 (MQ=255)

                                 ggATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGc                                                        <  1:1227599/93‑1 (MQ=255)

                                  gATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCa                                                       >  2:3585346/1‑93 (MQ=255)

                                   aTGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAg                                                      <  1:1732953/93‑1 (MQ=255)

                                     gcgcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc                                                    >  2:6597147/1‑93 (MQ=255)

                                       gcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         >  2:3439773/1‑70 (MQ=255)

                                       gcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGc                                                  >  2:1119813/1‑93 (MQ=255)

                                       gcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGc                                                  >  2:1314871/1‑93 (MQ=255)

                                          aTCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTca                                               >  1:2901214/1‑93 (MQ=255)

                                             aCGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaaca                                            >  1:6374155/1‑93 (MQ=255)

                                             aCGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaaca                                            >  1:3381478/1‑93 (MQ=255)

                                               gCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCaacaac                                          <  2:4461398/93‑1 (MQ=255)

                                               gCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCaacaac                                          >  2:1776979/1‑93 (MQ=255)

                                                cTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACg                                         >  1:2254754/1‑93 (MQ=255)

                                                cTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACg                                         <  1:95506/93‑1 (MQ=255)

                                                 tGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACCACgg                                        >  2:5879622/1‑93 (MQ=255)

                                                 tGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACgg                                        <  1:3467202/93‑1 (MQ=255)

                                                   tCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTc                                      >  2:3783129/1‑93 (MQ=255)

                                                    ccTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCt                                     <  1:38388/93‑1 (MQ=255)

                                                     cTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTg                                    >  2:5235247/1‑93 (MQ=255)

                                                       gCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGcc                                  >  2:5563010/1‑93 (MQ=255)

                                                         acaAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAg                                <  2:2605994/93‑1 (MQ=255)

                                                          caAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGt                               <  2:2887319/93‑1 (MQ=255)

                                                           aaaaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTa                              <  1:5201235/93‑1 (MQ=255)

                                                             aaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAct                            >  1:4992599/1‑93 (MQ=255)

                                                              aTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActg                           >  1:4407942/1‑93 (MQ=255)

                                                               tCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActgc                          >  1:4556983/1‑93 (MQ=255)

                                                                   tAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTa                      >  1:1302069/1‑93 (MQ=255)

                                                                      aagtaagtACTGACTTCGCCCGTGCGCTAAGTGATTCTTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACg                   <  1:4154648/93‑1 (MQ=255)

                                                                      aagtaagtACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACg                   <  2:2786150/93‑1 (MQ=255)

                                                                       agtaagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACgg                  >  1:1121375/1‑93 (MQ=255)

                                                                       agtaagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACgg                  <  2:775858/93‑1 (MQ=255)

                                                                         taagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTg                <  1:1985877/93‑1 (MQ=255)

                                                                           agtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGaa              >  2:6019268/1‑93 (MQ=255)

                                                                            gtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAag             >  1:2066589/1‑93 (MQ=255)

                                                                             taCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAaga            >  2:4303112/1‑93 (MQ=255)

                                                                             taCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAaga            <  2:5436498/93‑1 (MQ=255)

                                                                              aCTGACTTCGCCCGTGCGCTCAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagag           >  2:1493262/1‑93 (MQ=255)

                                                                               cTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagaga          <  1:3216132/93‑1 (MQ=255)

                                                                                tggcTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagagag         <  1:180430/90‑1 (MQ=255)

                                                                                 gACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGc        <  1:5753016/93‑1 (MQ=255)

                                                                                 gACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGc        <  2:3908626/93‑1 (MQ=255)

                                                                                   cTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCGAGTACTGCTGTAACGGTGAAGAGCGCgg      >  2:2239399/1‑93 (MQ=255)

                                                                                   cTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCgg      <  2:5155251/93‑1 (MQ=255)

                                                                                   cTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCgg      >  1:2799963/1‑93 (MQ=255)

                                                                                    ttCGCCCGTGCTCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGc     >  2:23060/1‑93 (MQ=255)

                                                                                    ttCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGc     >  2:169901/1‑93 (MQ=255)

                                                                                     tCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCt    <  1:3059332/93‑1 (MQ=255)

                                                                                     tCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCt    <  1:1942000/93‑1 (MQ=255)

                                                                                      cGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCtt   <  2:385894/93‑1 (MQ=255)

GGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTC  >  NC_000913/3197101‑3197280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`