Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,203,865 C→T L11L (TTG→TTA)  ygiP ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,203,8650CT88.9% 873.2 / 70.3 280L11L (TTG→TTA) ygiPputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base T (111/138);  total (128/152)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

Only 100 of 285 total aligned reads displayed.

TGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3203773‑3203956
                                                                                            |                                                                                           
tGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGc                                                                                             >  2:4309167/1‑93 (MQ=255)
    tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAgc                                                                                         >  1:4806689/1‑92 (MQ=255)
      cGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCt                                                                                       <  1:1113205/93‑1 (MQ=255)
      cGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCt                                                                                       >  1:1665775/1‑93 (MQ=255)
       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      >  1:1576797/1‑93 (MQ=255)
       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      <  2:6431438/93‑1 (MQ=255)
       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      <  2:4546538/93‑1 (MQ=255)
         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:2159667/93‑1 (MQ=255)
         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:3277681/93‑1 (MQ=255)
         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:493515/93‑1 (MQ=255)
         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:4959842/93‑1 (MQ=255)
         ttGGTGACAAATGCAGGCGGCTGCCCAAGCGTCGCCGCTGCGTCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  1:510726/93‑1 (MQ=255)
          tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTgg                                                                                   >  2:502709/1‑93 (MQ=255)
          tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTgg                                                                                   >  1:1312783/1‑93 (MQ=255)
           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  <  2:6148726/93‑1 (MQ=255)
           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  >  1:3354826/1‑93 (MQ=255)
           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGCGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  >  2:785470/1‑93 (MQ=255)
            gTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCt                                                                                 <  2:1251396/93‑1 (MQ=255)
             tGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTa                                                                                >  2:5873837/1‑93 (MQ=255)
             ggACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTa                                                                                <  2:1885178/92‑1 (MQ=255)
              gACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaa                                                                               >  1:3698513/1‑93 (MQ=255)
               aCAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaaa                                                                              <  1:999023/93‑1 (MQ=255)
               aCAAAAGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaaa                                                                              <  1:1556758/93‑1 (MQ=255)
                cAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAg                                                                             <  1:2808262/93‑1 (MQ=255)
                 aaaTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAgg                                                                            <  2:4803062/93‑1 (MQ=255)
                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGcc                                                                          >  2:6602869/1‑93 (MQ=255)
                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGcc                                                                          >  1:4947990/1‑93 (MQ=255)
                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGcc                                                                          >  1:2516766/1‑93 (MQ=255)
                     gCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAg                                                                        <  2:1145755/93‑1 (MQ=255)
                     gCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAg                                                                        <  2:5550471/93‑1 (MQ=255)
                      cAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGc                                                                       >  2:1050569/1‑93 (MQ=255)
                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCTCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCt                                                                      <  2:1410974/93‑1 (MQ=255)
                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCt                                                                      >  1:3195167/1‑93 (MQ=255)
                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCt                                                                      >  2:3903743/1‑93 (MQ=255)
                          cGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTAtt                                                                   >  2:2496815/1‑93 (MQ=255)
                          cGTCTCCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAtt                                                                   <  1:5226423/93‑1 (MQ=255)
                           gTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                  >  1:4102400/1‑93 (MQ=255)
                            tCTGCCCGAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCa                                                                 <  1:3965458/93‑1 (MQ=255)
                               gCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCa                                                              <  1:2987176/93‑1 (MQ=255)
                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGTCCAGCTATTCAGCATc                                                            >  1:642713/1‑93 (MQ=255)
                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATc                                                            >  2:957113/1‑93 (MQ=255)
                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATc                                                            >  1:4206085/1‑93 (MQ=255)
                                     gCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTa                                                        >  2:5355475/1‑93 (MQ=255)
                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    >  1:2723279/1‑93 (MQ=255)
                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    <  2:4735300/93‑1 (MQ=255)
                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    <  2:2260658/93‑1 (MQ=255)
                                          gccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTAt                                                   >  1:5180447/1‑93 (MQ=255)
                                           ccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATc                                                  >  2:3007838/1‑93 (MQ=255)
                                           ccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATc                                                  <  1:6294928/93‑1 (MQ=255)
                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 <  1:1234876/93‑1 (MQ=255)
                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 >  2:3544012/1‑93 (MQ=255)
                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 <  2:4121449/93‑1 (MQ=255)
                                             gcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCg                                                >  1:2067752/1‑93 (MQ=255)
                                              cTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGGCAGCTAGTCAGCATCGGTCATTATCGGc                                               >  2:1587965/1‑93 (MQ=255)
                                              cTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGc                                               <  2:1708474/93‑1 (MQ=255)
                                               tGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              <  1:4438319/93‑1 (MQ=255)
                                               tGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              <  1:253191/93‑1 (MQ=255)
                                                gcggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAg                                             <  1:6206770/93‑1 (MQ=255)
                                                gcggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAg                                             <  1:2017155/93‑1 (MQ=255)
                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            >  1:3078978/1‑93 (MQ=255)
                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            <  2:1719096/93‑1 (MQ=255)
                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            >  2:2811228/1‑93 (MQ=255)
                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            <  1:105653/93‑1 (MQ=255)
                                                  gtcgcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           <  1:5996463/91‑1 (MQ=255)
                                                  ggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           <  2:3671447/93‑1 (MQ=255)
                                                    cgcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgt                                         <  2:89014/93‑1 (MQ=255)
                                                     gcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgtg                                        >  2:6002925/1‑93 (MQ=255)
                                                      cTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGa                                       <  2:5726083/93‑1 (MQ=255)
                                                      cTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGa                                       >  2:3719180/1‑93 (MQ=255)
                                                       tAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGAt                                      <  1:3886830/93‑1 (MQ=255)
                                                         aaaCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATaa                                    >  2:2394407/1‑93 (MQ=255)
                                                          aaCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAg                                   >  2:2265041/1‑93 (MQ=255)
                                                           aCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgc                                  >  2:3211273/1‑93 (MQ=255)
                                                            cTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgcg                                 >  2:2168617/1‑93 (MQ=255)
                                                                 gCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtg                            <  2:5277754/93‑1 (MQ=255)
                                                                  cTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtgt                           >  1:729639/1‑93 (MQ=255)
                                                                   tgtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTa                          >  1:868939/1‑93 (MQ=255)
                                                                   tgtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTa                          <  1:5693463/93‑1 (MQ=255)
                                                                    gtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAt                         <  2:6322120/93‑1 (MQ=255)
                                                                    gtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAt                         <  1:3437381/93‑1 (MQ=255)
                                                                     tgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAtt                        >  1:2196972/1‑93 (MQ=255)
                                                                     tgAACGATTTCCACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAtt                        <  2:2360342/93‑1 (MQ=255)
                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  2:1394131/93‑1 (MQ=255)
                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  2:3099992/93‑1 (MQ=255)
                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       >  2:2687784/1‑93 (MQ=255)
                                                                         cGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGc                    <  1:2321115/93‑1 (MQ=255)
                                                                          gATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCa                   >  2:152529/1‑93 (MQ=255)
                                                                           aTTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCaa                  >  1:5382940/1‑93 (MQ=255)
                                                                               cTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACa              <  1:3487207/93‑1 (MQ=255)
                                                                                 aCCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACAtt            >  2:2094775/1‑93 (MQ=255)
                                                                                  ccAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTg           >  2:4833128/1‑93 (MQ=255)
                                                                                   cAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          <  1:6364835/93‑1 (MQ=255)
                                                                                   cAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          >  1:2050715/1‑93 (MQ=255)
                                                                                   cAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          <  1:3258264/93‑1 (MQ=255)
                                                                                    aGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGcc         <  1:1363840/93‑1 (MQ=255)
                                                                                    aGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGcc         >  2:3347650/1‑93 (MQ=255)
                                                                                       aCCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCAtt      <  2:6484673/93‑1 (MQ=255)
                                                                                       aCCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCAtt      >  2:2233226/1‑93 (MQ=255)
                                                                                         cTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTcc    <  2:644907/93‑1 (MQ=255)
                                                                                          tGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTccc   <  1:5435864/93‑1 (MQ=255)
                                                                                            |                                                                                           
TGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3203773‑3203956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑