BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,592,609	A→G	L168P (CTG→CCG)	livM ←	LivM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,592,609	0	A	G	87.0%	610.9 / 75.3	230	L168P (CTG→CCG)	livM	LivM
Reads supporting (aligned to +/- strand): ref base A (16/14); new base G (96/104); total (112/118)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 269 total aligned reads displayed.

GGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGC  >  NC_000913/3592518‑3592701

ggTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCggcag                                                                                             >  2:3214956/1‑93 (MQ=255)

  tAACGATCGCCATATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCggcgggc                                                                                           >  2:3275865/1‑93 (MQ=255)

  tAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCggcgggc                                                                                           <  1:1367656/93‑1 (MQ=255)

    aCGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGgcgggcag                                                                                         <  1:4203906/93‑1 (MQ=255)

     cGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGcgggcagg                                                                                        <  2:3941167/93‑1 (MQ=255)

       aTCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCACTCGGCAGGCAAGTc                                                                                      >  1:5579865/1‑93 (MQ=255)

        tCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTcc                                                                                     <  2:4145496/93‑1 (MQ=255)

         cGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCa                                                                                    <  1:1926054/93‑1 (MQ=255)

         cGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCa                                                                                    <  1:2395961/93‑1 (MQ=255)

         cGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCa                                                                                    <  2:4431356/93‑1 (MQ=255)

          gCCAGATAGTCACCGCGCAACGGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAg                                                                                   <  2:2438362/93‑1 (MQ=255)

          gCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAg                                                                                   >  1:6217549/1‑93 (MQ=255)

          cccAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAg                                                                                   <  2:1229679/92‑1 (MQ=255)

           ccAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGa                                                                                  <  2:5711039/93‑1 (MQ=255)

           ccAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGa                                                                                  >  2:3311920/1‑93 (MQ=255)

            cAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGaa                                                                                 >  1:778761/1‑93 (MQ=255)

            cAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGAAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGaa                                                                                 <  2:2580974/93‑1 (MQ=255)

             aGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAg                                                                                >  1:673611/1‑93 (MQ=255)

              gATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGGCCAGAAGc                                                                               >  2:640749/1‑93 (MQ=255)

                tAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGccc                                                                             >  1:1562542/1‑93 (MQ=255)

                tAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGccc                                                                             >  2:3407917/1‑93 (MQ=255)

                 aGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCa                                                                            <  1:4448276/93‑1 (MQ=255)

                 aGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCa                                                                            >  1:2812645/1‑93 (MQ=255)

                 aGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCa                                                                            <  1:5476192/93‑1 (MQ=255)

                   tCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAg                                                                          >  2:5850877/1‑93 (MQ=255)

                   tCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAg                                                                          >  1:6622950/1‑93 (MQ=255)

                   tCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAg                                                                          <  2:4234561/93‑1 (MQ=255)

                          gcAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAAt                                                                   <  1:3322738/93‑1 (MQ=255)

                           cAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCGAGAAGCCCAAGCCGTAATa                                                                  <  1:6499248/93‑1 (MQ=255)

                           cAAACGCAGCACCGGAAAACCGAGCAGGAACCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATa                                                                  <  2:2960508/93‑1 (MQ=255)

                            aaccGCAGCACCGGAAAACGGAGCAGAAACCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAg                                                                 <  1:4683129/90‑1 (MQ=255)

                            aaaCGCAGCACGGAAAACCCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAg                                                                 <  2:3518768/93‑1 (MQ=255)

                            aaaCGCAGCACCGGAAAGCCGAGCAGAAAACCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCGGGTCCAGAAGCCCAAGCCGTAATAg                                                                 <  1:3676301/93‑1 (MQ=255)

                            aaaCGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAg                                                                 >  1:3549791/1‑93 (MQ=255)

                             aaCGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGt                                                                >  2:5391653/1‑93 (MQ=255)

                             aaCGCAGCACCGGAAAACCAAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGt                                                                <  2:4695928/93‑1 (MQ=255)

                               cGCAGCACCGAAAAACCGACCAGGAGCCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGAAATAGTGa                                                              <  1:6455117/93‑1 (MQ=255)

                                gcagcgCGGGAAAACGGAGCAGGAAGCCCGCCGCCTCTGCCATTAATCCAGCAAGCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGAt                                                             <  1:1478463/93‑1 (MQ=255)

                                 cagcaCCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGAtt                                                            >  1:4495740/1‑93 (MQ=255)

                                 cagcaCCGGAAAACCAAGCAGAAACCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGAtt                                                            <  1:4396938/93‑1 (MQ=255)

                                  agcaCCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCCGGTCCAGAAGCCCAACCCGTACTAGTGGTTg                                                           >  1:5701179/1‑93 (MQ=255)

                                   gcaCCGGAAAACCGGCCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATGGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGa                                                          <  1:5715090/93‑1 (MQ=255)

                                   gcaCCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGa                                                          <  1:3366440/93‑1 (MQ=255)

                                   acaCCGGAAACCCGAGCAGAAACCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGa                                                          <  1:2151322/92‑1 (MQ=255)

                                     aCCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGagc                                                        >  1:1483606/1‑93 (MQ=255)

                                      ccGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGGAGCCCAAGCCGTAATAGTGATTGagca                                                       >  2:2764754/1‑93 (MQ=255)

                                      ccGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGagca                                                       >  2:1676610/1‑93 (MQ=255)

                                      ccGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGagca                                                       >  1:2682424/1‑93 (MQ=255)

                                      ccGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGagca                                                       <  1:3820657/93‑1 (MQ=255)

                                        ggAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGagcagc                                                     <  2:1148157/93‑1 (MQ=255)

                                        gaaaacCCGAGCAGAAACCCCCCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCAAGAAGCCCAAGCCGTAATAGTGATTGagcagc                                                     <  1:596426/91‑1 (MQ=255)

                                             aCCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCaaa                                                >  1:961771/1‑93 (MQ=255)

                                               cGAGCAGGAACCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAg                                              <  1:1126441/93‑1 (MQ=255)

                                                gAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAgt                                             >  2:2295157/1‑93 (MQ=255)

                                                 aGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGGAAAAgtg                                            >  2:94295/1‑93 (MQ=255)

                                                  gCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAgtgt                                           >  2:2663810/1‑93 (MQ=255)

                                                  gCAGAAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAgtgt                                           <  1:659124/93‑1 (MQ=255)

                                                   gaGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTa                                          <  1:4139296/92‑1 (MQ=255)

                                                    aGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGGCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTaa                                         >  2:3721935/1‑93 (MQ=255)

                                                     ggAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAg                                        >  2:1130506/1‑93 (MQ=255)

                                                     ggAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAg                                        >  1:6132511/1‑93 (MQ=255)

                                                     ggAAGCCCCCCCCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCAAGAAGCCAAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAg                                        <  2:3464946/93‑1 (MQ=255)

                                                     gaaaGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAg                                        <  2:5020037/91‑1 (MQ=255)

                                                      gAAGCCCGCCGCGGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCAAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAgc                                       <  2:3122870/93‑1 (MQ=255)

                                                       aaGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAgcg                                      >  1:2159074/1‑93 (MQ=255)

                                                        aGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAgcgc                                     <  2:6013661/93‑1 (MQ=255)

                                                        aGCCCCCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAgcgc                                     <  1:5313152/93‑1 (MQ=255)

                                                         gCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGcc                                    >  2:318221/1‑93 (MQ=255)

                                                           ccgccgccgCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGa                                  <  2:6532558/93‑1 (MQ=255)

                                                           ccgccgccgCTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGa                                  <  1:877263/93‑1 (MQ=255)

                                                            cgccgccgcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGAt                                 <  1:1769984/93‑1 (MQ=255)

                                                              ccgccgcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATgg                               <  1:2995673/93‑1 (MQ=255)

                                                                 ccgcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGt                            >  1:5032781/1‑93 (MQ=255)

                                                                 ccgcTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGt                            <  1:3988818/93‑1 (MQ=255)

                                                                 ccgcTGCAATTAATCGAGCAATCGGCAGGCAGGTCCAGAAGCCAAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGt                            <  2:1282490/93‑1 (MQ=255)

                                                                  cgcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTa                           <  2:406499/93‑1 (MQ=255)

                                                                   gcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaa                          >  2:5615409/1‑93 (MQ=255)

                                                                   gcTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaa                          >  2:865814/1‑93 (MQ=255)

                                                                    cTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaaa                         <  2:1762440/93‑1 (MQ=255)

                                                                    cTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaaa                         >  1:2527297/1‑93 (MQ=255)

                                                                    cTGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaaa                         >  1:429864/1‑93 (MQ=255)

                                                                     tGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaaaa                        >  2:358001/1‑93 (MQ=255)

                                                                     tGCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGGAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGAGGGCGTaaaa                        >  2:1381421/1‑93 (MQ=255)

                                                                      gCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTaaaaa                       >  1:5850333/1‑93 (MQ=255)

                                                                      gCCATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGAGTAAGCGCCGATGGCGTaaaaa                       >  2:6444731/1‑93 (MQ=255)

                                                                       ccATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAAc                      >  1:2717509/1‑93 (MQ=255)

                                                                       ccATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAAc                      <  2:802284/93‑1 (MQ=255)

                                                                       ccATTAATCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAAc                      <  1:910784/93‑1 (MQ=255)

                                                                        cATTAATCCAGCAATCGGCAGGCAGGTCTAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAAcc                     >  2:1353715/1‑93 (MQ=255)

                                                                             aTCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGt                >  1:3096443/1‑93 (MQ=255)

                                                                              tCCAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGGAATAGGGAATGAGCAGCCCAAAAGTGTAAGCGCCGATGGCGTAAAAAccgccgga               >  1:1071853/1‑91 (MQ=255)

                                                                               ccAGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGAGCCGATGGCGTAAAAACCGCCGTAc              >  1:2043248/1‑93 (MQ=255)

                                                                                cAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTAcc             >  1:4958851/1‑93 (MQ=255)

                                                                                 aGCAATCGGGGGGCAGGTCGAGAGGCCTAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTAccc            <  2:4867632/93‑1 (MQ=255)

                                                                                 aGCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTAccc            >  1:5619448/1‑93 (MQ=255)

                                                                                  gCAATCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTAcccc           >  2:678237/1‑93 (MQ=255)

                                                                                    aaTCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCccag         >  1:2301073/1‑93 (MQ=255)

                                                                                    aaTCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCccag         >  1:6042553/1‑93 (MQ=255)

                                                                                    aaTCGGCGGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCccag         >  2:6458378/1‑93 (MQ=255)

                                                                                        ggcgggcaGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCccagcacc     <  2:1910262/93‑1 (MQ=255)

GGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCCATTAATCCAGCAATCGGCAGGCAGGTCCAGAAGCCCAAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGC  >  NC_000913/3592518‑3592701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`