BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	1125.3 / NA	286	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (142/144); total (142/144)

Only 100 of 292 total aligned reads displayed.

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAAT  >  NC_000913/3754220‑3754399

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                       >  2:4437937/1‑93 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                       >  1:6427867/1‑93 (MQ=255)

   aGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                                      >  1:3633240/1‑93 (MQ=255)

   aGCTATCACGAATTGACGGGCAAGGGTTACAGAGATAGTTGAATAGTCTGTTAACAATTAGTCAGACGTTCTGGAAATGATGATTACATCGCt                                                                                      <  2:1064301/93‑1 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                     >  2:5991716/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                     <  1:3168086/93‑1 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    <  1:4834550/93‑1 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    >  1:3382172/1‑93 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                   >  1:3988801‑M1/1‑92 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                   >  1:2354054‑M1/1‑92 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  >  2:123735‑M1/1‑91 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  <  1:5920456‑M1/93‑3 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  <  2:6180339‑M1/93‑3 (MQ=255)

        tCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                                                 >  1:4117935‑M1/1‑90 (MQ=255)

         cACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcga                                                                                <  1:1002668‑M1/93‑5 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                               <  2:4956494‑M1/93‑6 (MQ=255)

              aTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaat                                                                           <  2:3129300‑M1/93‑10 (MQ=255)

               tttACGGGCAGGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          <  2:291827‑M1/93‑11 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          >  2:873182‑M1/1‑83 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          <  2:6281911‑M1/93‑11 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgggataaatcgc                                                                        >  2:259251‑M1/1‑81 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                                        <  1:2114179‑M1/93‑13 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                                        <  1:2657096‑M1/93‑13 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                                  >  2:5181669‑M1/1‑75 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                                 >  2:2999618‑M1/1‑74 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               <  2:3998760‑M1/93‑22 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                             <  2:3533419‑M1/93‑24 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                             <  2:2578314‑M1/93‑24 (MQ=255)

                              tACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcg                                                           >  2:2301094‑M1/1‑68 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGTCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  1:1748167‑M1/93‑27 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  2:3182345‑M1/93‑27 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  1:918094‑M1/93‑27 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  1:5968029‑M1/93‑27 (MQ=255)

                                cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                                         >  1:2745755‑M1/1‑66 (MQ=255)

                                cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAAGGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                                         <  1:6379683‑M1/93‑28 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        <  2:1119062‑M1/93‑29 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        <  1:2890162‑M1/93‑29 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        >  1:206339‑M1/1‑65 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       >  1:4760407‑M1/1‑64 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       >  1:6134686‑M1/1‑64 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       >  2:547689‑M1/1‑64 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTGGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcaataaatcgccacaatttcttcgtgag                                                       <  2:2248525‑M1/93‑30 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  2:1005374‑M1/93‑32 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:1179963‑M1/93‑32 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     >  1:4582379‑M1/1‑62 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:5277847‑M1/93‑32 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     >  1:5380366‑M1/1‑62 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:3515173‑M1/93‑32 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                                  <  2:766068‑M1/93‑35 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                                  >  2:4239390‑M1/1‑59 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                                  <  2:6530481‑M1/93‑35 (MQ=255)

                                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                                                 <  2:3758101‑M1/93‑36 (MQ=255)

                                           gAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctgga                                              <  2:5683412‑M1/93‑39 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                             <  1:464728‑M1/93‑40 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                           >  2:1783848‑M1/1‑52 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                          >  2:1329873‑M1/1‑51 (MQ=255)

                                                aGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                                         >  2:349228‑M1/1‑50 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                   <  2:6261608‑M1/93‑50 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                   >  2:3569977‑M1/1‑44 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                   >  1:5033677‑M1/1‑44 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  <  2:5499685‑M1/93‑51 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  >  2:6075451‑M1/1‑43 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  >  2:4462959‑M1/1‑43 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  >  1:4733286‑M1/1‑43 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaa                                 <  1:2427587‑M1/93‑52 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccaaaatttcttcgtgagttgcctggatcgggttagtaaa                                 >  1:1304202‑M1/1‑42 (MQ=255)

                                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc                               >  1:5659999‑M1/1‑40 (MQ=255)

                                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc                              >  1:3882526‑M1/1‑39 (MQ=255)

                                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcca                             <  2:1990883‑M1/93‑56 (MQ=255)

                                                             ttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccac                            >  1:6194914‑M1/1‑37 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                           >  1:6443586‑M1/1‑36 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                           <  1:4007939‑M1/93‑58 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctacgtgagttgcctggatcgggttagtaaagccaca                           <  1:6303415‑M1/93‑58 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                          >  2:3278632‑M1/1‑35 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                      >  2:47332‑M1/1‑31 (MQ=255)

                                                                    ccGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc                     <  1:5851108‑M1/93‑64 (MQ=255)

                                                                    ccGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc                     >  2:4644636‑M1/1‑30 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  1:825635‑M1/93‑65 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  2:4043694‑M1/93‑65 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  2:2080744‑M1/93‑65 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  1:5793497‑M1/93‑65 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   <  1:1422571‑M1/93‑66 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   <  1:1496541‑M1/93‑66 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  >  1:1940740‑M1/1‑27 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcg                >  1:4235574‑M1/1‑25 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               <  2:4126485‑M1/93‑70 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               <  1:5492720‑M1/93‑70 (MQ=255)

                                                                           ggAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagg              >  2:5577106‑M1/1‑23 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg             >  1:4428734‑M1/1‑22 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat          <  1:3442544‑M1/93‑75 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag       <  2:3700215‑M1/93‑78 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag       <  2:3918210‑M1/93‑78 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag       >  2:3441334‑M1/1‑16 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt      <  1:4925832‑M1/93‑79 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt      <  1:6508407‑M1/93‑79 (MQ=255)

                                                                                     atTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcg    >  2:4572550‑M1/1‑13 (MQ=255)

                                                                                     atTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcg    <  2:2020508‑M1/93‑81 (MQ=255)

                                                                                      ttACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgc   >  2:1448951‑M1/1‑12 (MQ=255)

                                                                                       tACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgcc  <  2:5900118‑M1/93‑83 (MQ=255)

                                                                                       tACATCGCTGCgcgataaaacgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgcc  <  2:1880899‑M1/93‑83 (MQ=255)

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAAT  >  NC_000913/3754220‑3754399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`