Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,765,203	A→T	Q119L (CAG→CTG)	yibJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,765,203	0	A	T	100.0%	112.2 / NA	35	Q119L (CAG→CTG)	yibJ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/22);  total (13/22)

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292                    |                                                                                          cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  <  1:5081659/93‑1 (MQ=9) cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  <  2:5458815/93‑1 (MQ=9)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:1100286/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  1:2559568/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  1:3113946/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:2460160/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  1:3543353/93‑1 (MQ=12)  gCTATTATGACCCGCTGCTGGGGCGGTATAGCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:1328909/93‑1 (MQ=255)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:1741127/1‑93 (MQ=255)    tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:2012807/1‑93 (MQ=255)     attatGACCTGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              <  2:5319818/93‑1 (MQ=255)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              >  1:5965036/1‑93 (MQ=255)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              >  2:664271/1‑93 (MQ=255)     attatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              >  1:1367165/1‑93 (MQ=255)      ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:1724787/1‑93 (MQ=255)      ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:5979927/1‑93 (MQ=255)        atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  2:1569710/93‑1 (MQ=255)        atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  1:1549359/1‑93 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:75145/93‑1 (MQ=255)         tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:301021/93‑1 (MQ=255)         tGACCCGCTGCTGGGGAGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  2:1572744/1‑93 (MQ=255)          gACCCGGTGCTGGGGCGGTATATCACTGAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         <  2:1075690/93‑1 (MQ=14)          gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         >  2:5412974/1‑93 (MQ=255)          gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         <  1:4167989/93‑1 (MQ=255)           aCCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATg        <  2:3413153/93‑1 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       >  2:5833834/1‑93 (MQ=255)            cccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  1:3114294/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  2:878110/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:2277346/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  2:6533208/93‑1 (MQ=255)             ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:4224000/93‑1 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:6012707/93‑1 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:4009415/93‑1 (MQ=255)              cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:5781400/93‑1 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  2:3744566/93‑1 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  1:2680052/1‑93 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  2:4963830/93‑1 (MQ=255)               gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    >  1:3377428/1‑93 (MQ=255)                ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   >  1:2088963/1‑93 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:5709916/1‑93 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:1147589/93‑1 (MQ=255)                 tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  1:6461636/1‑93 (MQ=255)                    |                                                                                          CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑