Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,075,331 A→G L101L (TTA→TTG)  yiiD → putative acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,075,3310AG84.1% 660.5 / 114.8 272L101L (TTA→TTG) yiiDputative acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (26/17);  new base G (116/112);  total (143/129)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 279 total aligned reads displayed.

GGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGT  >  NC_000913/4075241‑4075422
                                                                                          |                                                                                           
     cGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGc                                                                                      <  1:574439/93‑1 (MQ=255)
        tAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGa                                                                                   <  2:1915726/93‑1 (MQ=255)
         aGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGAt                                                                                  <  2:5703673/93‑1 (MQ=255)
         aGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGAt                                                                                  >  1:5542286/1‑93 (MQ=255)
         aGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGAt                                                                                  <  1:1407761/93‑1 (MQ=255)
         aGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGAt                                                                                  <  2:5979562/93‑1 (MQ=255)
         aGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGAt                                                                                  <  2:3970590/93‑1 (MQ=255)
          ggCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACACAGGGTTGTGCACGCTGATg                                                                                 >  1:5945943/1‑93 (MQ=255)
          ggCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGTACGCTGATg                                                                                 <  2:3805231/93‑1 (MQ=255)
          ggCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATg                                                                                 <  2:5866615/93‑1 (MQ=255)
          ggCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATg                                                                                 <  1:6115456/93‑1 (MQ=255)
           gCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATgg                                                                                >  1:119414/1‑93 (MQ=255)
            ccGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGc                                                                               <  1:6611792/93‑1 (MQ=255)
            ccGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGc                                                                               <  2:2004332/93‑1 (MQ=255)
              gACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGa                                                                             >  1:1858113/1‑93 (MQ=255)
              gACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGa                                                                             <  1:3453224/93‑1 (MQ=255)
              gACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCACGACGTGCAGGACCAAGGGTTAGGCACGCTGATgcgga                                                                             >  2:4928195/1‑89 (MQ=255)
               aCTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGAt                                                                            <  2:4834619/93‑1 (MQ=255)
               aCTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGAt                                                                            <  1:2019802/93‑1 (MQ=255)
               aCTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGAt                                                                            <  2:2985391/93‑1 (MQ=255)
                cTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATg                                                                           <  2:6186467/93‑1 (MQ=255)
                 tGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGa                                                                          >  2:3284486/1‑93 (MQ=255)
                 tGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGa                                                                          >  2:5125112/1‑93 (MQ=255)
                 tGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGa                                                                          <  2:4686106/93‑1 (MQ=255)
                  gTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGAc                                                                         >  2:1511421/1‑93 (MQ=255)
                  gTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGAc                                                                         >  2:2474460/1‑93 (MQ=255)
                  gTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGAc                                                                         >  1:3176896/1‑93 (MQ=255)
                     tatTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCt                                                                      <  1:1326898/93‑1 (MQ=255)
                     tatGAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCt                                                                      <  2:663742/93‑1 (MQ=255)
                       ttAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTgg                                                                    <  1:424146/93‑1 (MQ=255)
                        tAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGa                                                                   >  1:161128/1‑93 (MQ=255)
                         aaTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAg                                                                  <  1:997516/93‑1 (MQ=255)
                         aaTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAg                                                                  >  2:2863903/1‑93 (MQ=255)
                          aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGt                                                                 <  2:3164518/93‑1 (MQ=255)
                           ttccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTc                                                                >  2:1081990/3‑93 (MQ=255)
                              cGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGt                                                             <  2:2514286/93‑1 (MQ=255)
                                aCAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTgg                                                           <  1:6380672/93‑1 (MQ=255)
                                aCAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTgg                                                           >  2:1897898/1‑93 (MQ=255)
                                  aaTGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGgcg                                                         >  2:5633929/1‑93 (MQ=255)
                                  aaTGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGgcg                                                         >  2:6551868/1‑93 (MQ=255)
                                  aaTGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGgcg                                                         <  1:3009383/93‑1 (MQ=255)
                                      aaGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTc                                                     <  1:6166291/93‑1 (MQ=255)
                                       aGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCa                                                    <  2:1951963/93‑1 (MQ=255)
                                       aGCCTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCa                                                    <  1:35747/93‑1 (MQ=255)
                                        gCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAg                                                   >  1:29305/1‑93 (MQ=255)
                                         cGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAgg                                                  <  1:2487850/93‑1 (MQ=255)
                                          gTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGa                                                 >  1:2966140/1‑93 (MQ=255)
                                          gTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGa                                                 >  1:2140818/1‑93 (MQ=255)
                                           tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGaa                                                >  1:6474744/1‑93 (MQ=255)
                                           tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGaa                                                >  2:6409997/1‑93 (MQ=255)
                                           tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGGCGGTGGCGCGTCAGGaa                                                >  1:3401479/1‑93 (MQ=255)
                                           tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGaa                                                <  1:3902575/93‑1 (MQ=255)
                                              aTTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGc                                             >  1:2467127/1‑93 (MQ=255)
                                              aTTCGCTTTATGGCCGTTCATACCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTTAGGAAGGc                                             >  1:3306949/1‑93 (MQ=255)
                                               ttCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCg                                            <  2:5295793/93‑1 (MQ=255)
                                               ttCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCg                                            <  1:3208/93‑1 (MQ=255)
                                                tCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGt                                           >  2:349831/1‑93 (MQ=255)
                                                tCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGt                                           >  1:1110128/1‑93 (MQ=255)
                                                 cGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                          <  1:6452804/93‑1 (MQ=255)
                                                 cGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                          >  2:5033827/1‑93 (MQ=255)
                                                  gCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTa                                         <  2:732206/93‑1 (MQ=255)
                                                   cTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTaa                                        <  2:881348/93‑1 (MQ=255)
                                                   cTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTaa                                        >  1:6243544/1‑93 (MQ=255)
                                                       aTGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAgcgc                                    <  2:2105797/93‑1 (MQ=255)
                                                        tGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGcgcg                                   <  2:1726126/93‑1 (MQ=255)
                                                        tGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGAACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGcgcg                                   >  1:6557438/1‑93 (MQ=255)
                                                          gCCGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTg                                 >  1:3744498/1‑93 (MQ=255)
                                                            cGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAc                               <  1:4563935/93‑1 (MQ=255)
                                                            cGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAc                               <  2:1483213/93‑1 (MQ=255)
                                                            cGTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAc                               >  2:4272892/1‑93 (MQ=255)
                                                             gTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAcc                              >  2:5528991/1‑93 (MQ=255)
                                                             gTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAcc                              <  2:6061700/93‑1 (MQ=255)
                                                             gTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAcc                              >  2:4581761/1‑93 (MQ=255)
                                                             gTTCATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGAcc                              >  2:4150898/1‑93 (MQ=255)
                                                               tCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTTGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTg                            <  1:229450/93‑1 (MQ=255)
                                                                cATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGt                           >  2:4434256/1‑93 (MQ=255)
                                                                cATCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGt                           >  1:4179521/1‑93 (MQ=255)
                                                                 aTCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTa                          <  1:5216985/93‑1 (MQ=255)
                                                                 aTCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTa                          <  1:4714928/93‑1 (MQ=255)
                                                                  tCCCGACGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAg                         <  1:2721250/93‑1 (MQ=255)
                                                                   cgcGACGGGCAGGACAAAGAGTTGGGCACGGTGATGGCGATGACCCTGGAGTCGGTGGCGGGTCAGGAAGGCGTTAAGCGCGTGACCTGTAgc                        <  2:5488118/91‑1 (MQ=255)
                                                                       aCGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGccc                    >  1:5396583/1‑93 (MQ=255)
                                                                        cGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGGCCg                   >  1:2820561/1‑93 (MQ=255)
                                                                        cGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCg                   <  1:3713840/93‑1 (MQ=255)
                                                                        cGTGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCg                   >  2:35619/1‑93 (MQ=255)
                                                                          tGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                 >  2:6434594/1‑93 (MQ=255)
                                                                          tGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                 <  2:3381870/93‑1 (MQ=255)
                                                                          tGCAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                 <  2:1614545/93‑1 (MQ=255)
                                                                          tGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                 >  1:4383486/1‑93 (MQ=255)
                                                                            cAGGACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa               <  1:4990348/93‑1 (MQ=255)
                                                                              ggACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGa             <  1:1025738/93‑1 (MQ=255)
                                                                               gACAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAc            <  2:2358070/93‑1 (MQ=255)
                                                                                aCAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAcg           <  2:3648444/93‑1 (MQ=255)
                                                                                aCAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAcg           <  2:6599460/93‑1 (MQ=255)
                                                                                 cAAAGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAcgc          <  2:129517/93‑1 (MQ=255)
                                                                                    aGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCggg       >  2:4078339/1‑92 (MQ=255)
                                                                                    aGGGTTGGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGt       <  2:1650369/93‑1 (MQ=255)
                                                                                       gTTGGGCACGCTGATGGCGATGACGCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGa    <  1:6520747/93‑1 (MQ=255)
                                                                                       gTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGa    <  1:788138/93‑1 (MQ=255)
                                                                                        ttAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAg   >  2:2691284/1‑93 (MQ=255)
                                                                                          |                                                                                           
GGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGT  >  NC_000913/4075241‑4075422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑