Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,232,040 A→G H235R (CAC→CGC)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,232,0400AG86.3% 684.6 / 84.6 262H235R (CAC→CGC) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (16/20);  new base G (98/128);  total (114/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 271 total aligned reads displayed.

GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG  >  NC_000913/4231948‑4232129
                                                                                            |                                                                                         
   aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGt                                                                                        <  2:1866746/93‑1 (MQ=255)
   aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGt                                                                                        <  2:3925122/93‑1 (MQ=255)
    gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGtt                                                                                       >  2:2642745/1‑93 (MQ=255)
    gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGtt                                                                                       <  2:2770148/93‑1 (MQ=255)
     cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTg                                                                                      >  2:6042082/1‑93 (MQ=255)
      aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGc                                                                                     <  2:4245360/93‑1 (MQ=255)
      aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGGTGc                                                                                     >  2:5648905/1‑93 (MQ=255)
       tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCa                                                                                    <  1:2136133/93‑1 (MQ=255)
       tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGGTGCa                                                                                    >  2:468779/1‑93 (MQ=255)
            aaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAaca                                                                               >  1:6296019/1‑93 (MQ=255)
             aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAacac                                                                              >  2:1896771/1‑93 (MQ=255)
             aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAacac                                                                              >  2:4340350/1‑93 (MQ=255)
              ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACt                                                                             <  1:4146056/93‑1 (MQ=255)
              ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACt                                                                             >  2:4632393/1‑93 (MQ=255)
               cTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACtt                                                                            <  2:1335710/93‑1 (MQ=255)
                ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACttt                                                                           <  2:3448472/93‑1 (MQ=255)
                ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACttt                                                                           <  2:5838099/93‑1 (MQ=255)
                 tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTg                                                                          >  1:1074517/1‑93 (MQ=255)
                     cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggg                                                                      >  1:288067/1‑92 (MQ=255)
                     cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggc                                                                      <  2:4764014/93‑1 (MQ=255)
                      acTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggcg                                                                     >  2:4915652/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    >  1:766278/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    >  2:6441509/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    <  1:6246029/93‑1 (MQ=255)
                        tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCt                                                                   <  1:5841397/93‑1 (MQ=255)
                        tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCt                                                                   <  2:3191868/93‑1 (MQ=255)
                         cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                  >  1:5654067/1‑93 (MQ=255)
                            gAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTcc                                                               >  2:1418789/1‑93 (MQ=255)
                             aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTcca                                                              <  1:3783961/93‑1 (MQ=255)
                              aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccac                                                             <  1:3627434/93‑1 (MQ=255)
                              aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccac                                                             <  1:4592384/93‑1 (MQ=255)
                               aCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGGAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccacc                                                            >  2:338892/1‑93 (MQ=255)
                               aCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTccacc                                                            >  2:1591670/1‑93 (MQ=255)
                                    gACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGc                                                       <  1:5750267/93‑1 (MQ=255)
                                     aCCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGcc                                                      >  1:6363682/1‑93 (MQ=255)
                                       cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                    <  1:3004016/93‑1 (MQ=255)
                                        aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaaa                                                   >  2:2897158/1‑93 (MQ=255)
                                        aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaaa                                                   >  1:6627821/1‑93 (MQ=255)
                                          agCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGc                                                 <  1:650162/92‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  1:4645880/93‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  1:878594/93‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  2:1675123/93‑1 (MQ=255)
                                              caaaGCGCGCCGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  1:5575179/90‑1 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             >  1:5944378/1‑93 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  2:696133/93‑1 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  2:5759327/93‑1 (MQ=255)
                                               aTAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTg                                            >  2:2104522/1‑93 (MQ=255)
                                                tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                           >  2:6570107/1‑93 (MQ=255)
                                                tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                           <  2:5447168/93‑1 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          <  2:5168590/93‑1 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          >  2:2509272/1‑93 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGACAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          <  1:2686026/93‑1 (MQ=255)
                                                  gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                         >  1:2224435/1‑93 (MQ=255)
                                                  gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                         >  2:3494908/1‑93 (MQ=255)
                                                     cgctTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                      <  2:3589215/93‑1 (MQ=255)
                                                     cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGGCAAAGCCGTTGGCGAGt                                      >  2:3618987/1‑93 (MQ=255)
                                                     cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                      >  1:1393105/1‑93 (MQ=255)
                                                       cgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                    <  1:1248822/93‑1 (MQ=255)
                                                        gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                   <  1:1878718/93‑1 (MQ=255)
                                                        gTGACTGGTGCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                   <  1:3382180/93‑1 (MQ=255)
                                                         tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttttg                                  >  1:3267990/1‑91 (MQ=255)
                                                         tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                  >  2:4467509/1‑93 (MQ=255)
                                                          gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                 <  1:450716/93‑1 (MQ=255)
                                                          gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                 <  2:1274814/93‑1 (MQ=255)
                                                           aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                >  1:709214/1‑93 (MQ=255)
                                                            cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                               <  1:417264/93‑1 (MQ=255)
                                                             tGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                              <  2:1736023/93‑1 (MQ=255)
                                                             tGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                              >  2:5432763/1‑93 (MQ=255)
                                                              ggTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                             <  1:4957082/93‑1 (MQ=255)
                                                              ggTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                             >  1:409055/1‑93 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  1:154883/93‑1 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            >  1:169488/1‑93 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  1:4030937/93‑1 (MQ=255)
                                                               gTTCCTGAAAGCGCCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  2:3791838/93‑1 (MQ=255)
                                                                  ccTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                         <  2:1022260/93‑1 (MQ=255)
                                                                   cTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                        <  1:4547430/93‑1 (MQ=255)
                                                                    tGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTg                       >  1:2838187/1‑93 (MQ=255)
                                                                     gAAAGCGGCAGGGGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTGTTGCTACTGc                      >  1:3943908/1‑93 (MQ=255)
                                                                      aaaGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGcc                     <  2:6216393/93‑1 (MQ=255)
                                                                           ggCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                >  2:6483282/1‑93 (MQ=255)
                                                                            gCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATg               <  2:2032269/93‑1 (MQ=255)
                                                                             cAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGt              >  2:1933935/1‑93 (MQ=255)
                                                                             cAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGt              <  1:19474/93‑1 (MQ=255)
                                                                              aGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt             >  2:537770/1‑93 (MQ=255)
                                                                               ggTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc            <  1:856767/93‑1 (MQ=255)
                                                                               ggTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc            <  2:639226/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           <  1:290690/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           <  2:2404134/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           >  2:5871976/1‑93 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  1:4120803/93‑1 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  2:4086513/93‑1 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  2:4837649/93‑1 (MQ=255)
                                                                                 tgatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCCACATGTTCGa          >  1:5256477/1‑93 (MQ=255)
                                                                                  gatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg         >  1:2075742/1‑93 (MQ=255)
                                                                                  gatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg         >  1:5047365/1‑93 (MQ=255)
                                                                                   atgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt        >  1:2356766/1‑93 (MQ=255)
                                                                                     gaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTc      <  1:2451798/93‑1 (MQ=255)
                                                                                       aaaaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTg    >  1:3431063/1‑93 (MQ=255)
                                                                                        aaaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg   <  1:4279226/93‑1 (MQ=255)
                                                                                         aaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg  <  2:3525312/93‑1 (MQ=255)
                                                                                            |                                                                                         
GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG  >  NC_000913/4231948‑4232129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑