BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,388,615	A→G	V158A (GTG→GCG)	b4161 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,388,615	0	A	G	100.0%	1013.8 / NA	272	V158A (GTG→GCG)	b4161	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (142/130); total (142/130)

Only 100 of 276 total aligned reads displayed.

AATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA  >  NC_000913/4388525‑4388704

  tACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaat                                                                                       <  2:6343085/93‑1 (MQ=255)

  tACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaat                                                                                       >  1:2715587/1‑93 (MQ=255)

     gcgATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaataat                                                                                    <  2:4616656/93‑1 (MQ=255)

     gcgATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaataat                                                                                    >  1:205773/1‑93 (MQ=255)

       gATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCg                                                                                  <  1:5673054/93‑1 (MQ=255)

       gATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCg                                                                                  >  1:654018/1‑93 (MQ=255)

         tAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGc                                                                                >  2:5976519/1‑93 (MQ=255)

           aCCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTc                                                                              >  1:4776617/1‑93 (MQ=255)

            ccGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCa                                                                             >  2:240326/1‑93 (MQ=255)

            ccGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGGCTATCTTGTTGAGTACAATAATCGACTCa                                                                             >  2:6258064/1‑93 (MQ=255)

             cGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCaa                                                                            <  1:1079135/93‑1 (MQ=255)

             cGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCaa                                                                            <  2:1209480/93‑1 (MQ=255)

              gATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAAt                                                                           >  2:5259228/1‑93 (MQ=255)

              gATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAAt                                                                           >  2:4916520/1‑93 (MQ=255)

               atatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATc                                                                          >  2:2766115/1‑93 (MQ=255)

               atatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATc                                                                          <  2:312150/93‑1 (MQ=255)

                tatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCt                                                                         <  2:5830978/93‑1 (MQ=255)

                tatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCt                                                                         <  2:4165726/93‑1 (MQ=255)

                  ttGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGc                                                                       >  2:3423239/1‑93 (MQ=255)

                  ttGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGc                                                                       <  1:5100544/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  2:3061886/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  2:1346573/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  1:2754854/93‑1 (MQ=255)

                     cGGTAGATATCCATCTCCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAg                                                                    <  1:41529/93‑1 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  1:5727381/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  2:171241/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  2:1951765/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  1:2942800/1‑93 (MQ=255)

                       gTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGt                                                                  <  1:1671236/93‑1 (MQ=255)

                        tAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGtt                                                                 <  1:3299183/93‑1 (MQ=255)

                         aGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGgtt                                                                >  1:3789172/1‑93 (MQ=255)

                          gATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTc                                                               >  2:4641212/1‑93 (MQ=255)

                          gATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTc                                                               <  2:4494429/93‑1 (MQ=255)

                           atatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCg                                                              <  1:3694918/93‑1 (MQ=255)

                           atatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCg                                                              >  2:5484683/1‑93 (MQ=255)

                            tatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGc                                                             >  1:1774443/1‑93 (MQ=255)

                             atCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCa                                                            <  1:6278271/93‑1 (MQ=255)

                              tCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAg                                                           >  1:6045635/1‑93 (MQ=255)

                              tCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAg                                                           <  2:2599727/93‑1 (MQ=255)

                               ccATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAgg                                                          <  2:654421/93‑1 (MQ=255)

                               ccATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGGCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAgg                                                          >  1:2025737/1‑93 (MQ=255)

                                cATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGc                                                         <  2:5310058/93‑1 (MQ=255)

                                  tCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGcca                                                       >  2:1994027/1‑93 (MQ=255)

                                    tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacc                                                     >  1:5417746/1‑93 (MQ=255)

                                    tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacc                                                     >  1:978159/1‑93 (MQ=255)

                                     gCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacca                                                    >  2:5597920/1‑93 (MQ=255)

                                      cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAg                                                   >  2:3666436/1‑93 (MQ=255)

                                      cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAg                                                   <  1:2680618/93‑1 (MQ=255)

                                       tCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAgg                                                  <  2:2974747/93‑1 (MQ=255)

                                         gTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTa                                                >  2:1280590/1‑93 (MQ=255)

                                         gTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTa                                                >  1:3477470/1‑93 (MQ=255)

                                          ttGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTaa                                               <  2:453057/93‑1 (MQ=255)

                                           tGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAAc                                              <  2:5695228/93‑1 (MQ=255)

                                           tGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAAc                                              <  2:1412093/93‑1 (MQ=255)

                                             aCGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACgg                                            >  1:277591/1‑93 (MQ=255)

                                                aaCGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCg                                         <  1:302540/93‑1 (MQ=255)

                                                  cGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGat                                       <  1:1461834/93‑1 (MQ=255)

                                                    ccATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataa                                     >  2:1248220/1‑93 (MQ=255)

                                                     cATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataat                                    >  1:5175074/1‑93 (MQ=255)

                                                      aTGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataata                                   >  2:4359553/1‑93 (MQ=255)

                                                        gCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATAtt                                 <  2:6589551/93‑1 (MQ=255)

                                                        gCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATAtt                                 >  1:1558561/1‑93 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                <  1:4049433/93‑1 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                <  1:83126/93‑1 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                >  2:377393/1‑93 (MQ=255)

                                                          cTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGa                               <  1:2160777/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  1:5034221/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  1:3745126/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  2:5157980/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              >  2:1540228/1‑93 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           >  2:2299697/1‑93 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           <  2:5448501/93‑1 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           >  2:6085272/1‑93 (MQ=255)

                                                               tcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAc                          <  1:5982691/93‑1 (MQ=255)

                                                               tcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAc                          >  1:2541401/1‑93 (MQ=255)

                                                                cgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACa                         <  1:5372380/93‑1 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       <  2:1655062/93‑1 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       >  1:1859799/1‑93 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       >  2:2903174/1‑93 (MQ=255)

                                                                   ccAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCt                      >  1:4494987/1‑93 (MQ=255)

                                                                     agcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTcc                    <  2:6512647/93‑1 (MQ=255)

                                                                      gcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCg                   <  2:1769664/93‑1 (MQ=255)

                                                                      gcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCg                   <  2:6438037/93‑1 (MQ=255)

                                                                           tCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaa              >  2:3216130/1‑93 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             <  1:5299564/93‑1 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             >  2:3865933/1‑93 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             >  1:1836216/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:1249796/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:2412346/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:5642279/1‑93 (MQ=255)

                                                                               tCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATgg          >  2:2046740/1‑93 (MQ=255)

                                                                                cTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGc         >  1:1003024/1‑93 (MQ=255)

                                                                                cTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGc         <  1:6613745/93‑1 (MQ=255)

                                                                                 ttgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCg        >  2:1066572/1‑93 (MQ=255)

                                                                                 ttgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCg        >  2:4042242/1‑93 (MQ=255)

                                                                                  tgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCgg       >  1:2145041/1‑93 (MQ=255)

                                                                                  tgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCgg       >  1:157830/1‑93 (MQ=255)

                                                                                     tgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga    <  2:501439/93‑1 (MQ=255)

                                                                                     tgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga    <  1:4401722/93‑1 (MQ=255)

                                                                                      gAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAgac   >  2:3316895/1‑93 (MQ=255)

AATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA  >  NC_000913/4388525‑4388704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`