BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,416,484	A→G	H242H (CAT→CAC)	yjfR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,416,484	0	A	G	84.8%	661.1 / 99.2	258	H242H (CAT→CAC)	yjfR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (18/21); new base G (115/103); total (133/125)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA  >  NC_000913/4416392‑4416575

    cccccATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGtt                                                                                         <  1:6465975/91‑1 (MQ=255)

    cACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGtt                                                                                         <  2:2432880/93‑1 (MQ=255)

    cACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGtt                                                                                         <  2:1669555/93‑1 (MQ=255)

     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  1:1081488/1‑93 (MQ=255)

     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  2:2897495/1‑93 (MQ=255)

     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  1:4650735/1‑93 (MQ=255)

     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTc                                                                                        >  2:3521530/1‑93 (MQ=255)

     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTc                                                                                        >  2:4617453/1‑93 (MQ=255)

        cATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGtt                                                                                     >  2:5233469/1‑93 (MQ=255)

          tACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTAc                                                                                   <  1:2456787/93‑1 (MQ=255)

            cGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCg                                                                                 >  1:858062/1‑93 (MQ=255)

             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                <  1:1797524/93‑1 (MQ=255)

             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                >  2:389303/1‑93 (MQ=255)

             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                <  2:5921606/93‑1 (MQ=255)

             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                >  2:3042672/1‑93 (MQ=255)

              cagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGtc                                                                               >  1:2209956/1‑92 (MQ=255)

                 caTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCtt                                                                            <  1:4579095/93‑1 (MQ=255)

                   tatCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACGGTGCTTCg                                                                          >  2:4860071/1‑93 (MQ=255)

                   tatCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCg                                                                          >  2:4514794/1‑93 (MQ=255)

                    atCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGc                                                                         >  1:1190227/1‑93 (MQ=255)

                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       <  2:4787801/93‑1 (MQ=255)

                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       >  1:1541743/1‑93 (MQ=255)

                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       <  1:1457055/93‑1 (MQ=255)

                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       >  2:1015978/1‑93 (MQ=255)

                       ggCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCata                                                                      >  1:3907124/1‑93 (MQ=255)

                        gcgcTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCataa                                                                     >  1:5251577/1‑93 (MQ=255)

                          gcTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCataata                                                                   >  1:3757102/1‑93 (MQ=255)

                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  >  1:700304/1‑93 (MQ=255)

                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  <  2:4129251/93‑1 (MQ=255)

                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  >  1:535017/1‑93 (MQ=255)

                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  <  2:6469731/93‑1 (MQ=255)

                            tGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGt                                                                 <  2:4393743/93‑1 (MQ=255)

                             ggTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGtt                                                                <  2:923209/93‑1 (MQ=255)

                               tCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTag                                                              >  2:5319627/1‑93 (MQ=255)

                                cATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTaga                                                             <  2:6074751/93‑1 (MQ=255)

                                   tttGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGagta                                                          <  2:318634/93‑1 (MQ=255)

                                     tGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt                                                        >  2:110167/1‑93 (MQ=255)

                                     tGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt                                                        <  1:4205434/93‑1 (MQ=255)

                                      gTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTg                                                       <  2:1391608/93‑1 (MQ=255)

                                      gTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTg                                                       <  2:5708159/93‑1 (MQ=255)

                                       tCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTgg                                                      >  2:6028048/1‑93 (MQ=255)

                                        cGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTggg                                                     <  2:1240005/93‑1 (MQ=255)

                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   >  2:4193905/1‑93 (MQ=255)

                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   >  1:4740853/1‑93 (MQ=255)

                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   <  1:2421539/93‑1 (MQ=255)

                                           tGATACCGCGCGTGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  <  1:3685147/93‑1 (MQ=255)

                                           tGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  >  1:736707/1‑93 (MQ=255)

                                           tGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  >  2:2469870/1‑93 (MQ=255)

                                            gATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTc                                                 <  1:1720621/93‑1 (MQ=255)

                                             aTACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcg                                                >  2:5425877/1‑93 (MQ=255)

                                              tACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgc                                               <  1:5571654/93‑1 (MQ=255)

                                              tACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgc                                               <  1:3954786/93‑1 (MQ=255)

                                               actgcgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgcc                                              >  1:4274149/4‑93 (MQ=255)

                                               aCCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgcc                                              <  1:4994107/93‑1 (MQ=255)

                                                ccGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgccg                                             >  1:6416726/1‑93 (MQ=255)

                                                 cgcgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgccgc                                            >  2:2017938/1‑93 (MQ=255)

                                                   cgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtg                                          >  1:3452750/1‑93 (MQ=255)

                                                    gcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgt                                         >  1:2155813/1‑93 (MQ=255)

                                                    gcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgt                                         <  2:3136746/93‑1 (MQ=255)

                                                     cgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgtg                                        >  1:1627083/1‑93 (MQ=255)

                                                     cgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgtg                                        >  2:6631920/1‑93 (MQ=255)

                                                       ggTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGAt                                      >  2:3328554/1‑93 (MQ=255)

                                                        gTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATa                                     >  1:5809285/1‑93 (MQ=255)

                                                         ttttCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATAc                                    <  2:684511/93‑1 (MQ=255)

                                                           ttCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAg                                  <  1:5021974/93‑1 (MQ=255)

                                                            tCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAgg                                 <  2:3466784/93‑1 (MQ=255)

                                                            tCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAgg                                 <  2:4232513/93‑1 (MQ=255)

                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                >  1:1671352/1‑93 (MQ=255)

                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                <  1:2867299/93‑1 (MQ=255)

                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                <  1:2611727/93‑1 (MQ=255)

                                                              gCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGa                               <  1:5169146/93‑1 (MQ=255)

                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  1:604784/1‑93 (MQ=255)

                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:6308212/1‑93 (MQ=255)

                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:5081152/1‑93 (MQ=255)

                                                                cGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:2652522/1‑93 (MQ=255)

                                                                 gTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgcc                            <  1:2340571/93‑1 (MQ=255)

                                                                   tcGATCCTAACGCCACGTCGATCTGTTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgc                          <  1:4322964/92‑1 (MQ=255)

                                                                    cGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgcc                         <  1:5960256/93‑1 (MQ=255)

                                                                      aTCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAg                       <  2:3698533/93‑1 (MQ=255)

                                                                          tAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGt                   <  2:898067/93‑1 (MQ=255)

                                                                           aaCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtt                  <  2:258275/93‑1 (MQ=255)

                                                                            aCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGttt                 <  2:5035692/93‑1 (MQ=255)

                                                                            aCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGttt                 <  1:4080196/93‑1 (MQ=255)

                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                <  2:4727205/93‑1 (MQ=255)

                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                >  2:2219260/1‑93 (MQ=255)

                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                >  2:322594/1‑93 (MQ=255)

                                                                             cGCCACGTCGATCTGGTGTTCGTCACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                <  2:3615515/93‑1 (MQ=255)

                                                                              gCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCAGCCAGGCGTTTTg               >  1:3333583/1‑93 (MQ=255)

                                                                               ccACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGa              >  1:5827844/1‑93 (MQ=255)

                                                                                cACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGaa             <  1:3076823/93‑1 (MQ=255)

                                                                                   gTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAg          >  1:3609884/1‑93 (MQ=255)

                                                                                   gTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAg          >  1:1002658/1‑93 (MQ=255)

                                                                                     cGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGt        <  2:1906567/93‑1 (MQ=255)

                                                                                      gATCTGGTTTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTa       <  2:612998/93‑1 (MQ=255)

                                                                                      gATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTa       <  2:1548685/93‑1 (MQ=255)

                                                                                       aTCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAg      >  1:2551674/1‑93 (MQ=255)

                                                                                       aTCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTCAACAGGTAg      >  2:2511208/1‑93 (MQ=255)

                                                                                       aTCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAg      <  2:3990289/93‑1 (MQ=255)

                                                                                        tCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGt     <  1:451143/93‑1 (MQ=255)

                                                                                         cTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGtt    >  2:2775677/1‑93 (MQ=255)

GCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA  >  NC_000913/4416392‑4416575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`