BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,583,050	G→A	Q446* (CAA→TAA)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,583,050	0	G	A	99.6%	1014.0 / NA	261	Q446* (CAA→TAA)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (122/138); total (122/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG  >  NC_000913/4582960‑4583140

  tCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGt                                                                                        <  2:4569166/93‑1 (MQ=255)

   cAACCGCGCGGAGGCTATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGtt                                                                                       <  1:5599988/93‑1 (MQ=255)

   cAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGtt                                                                                       <  2:6440899/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  1:28390/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  2:2769586/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      >  1:1974741/1‑93 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  1:2453339/93‑1 (MQ=255)

      ccacgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCAGGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  2:2628433/90‑1 (MQ=255)

      ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  1:4221785/93‑1 (MQ=255)

      ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  2:1278883/93‑1 (MQ=255)

           cgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCg                                                                               >  1:3486930/1‑93 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                              <  1:4288228/93‑1 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                              <  1:3100552/93‑1 (MQ=255)

              aCGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGc                                                                            <  1:1837821/93‑1 (MQ=255)

               cGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCa                                                                           <  1:4976630/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                         <  2:6585914/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                         <  2:4371281/93‑1 (MQ=255)

                  cATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAttt                                                                        >  1:3876227/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACTGTTCGCTGCCGAGCATTTcc                                                                      >  2:6495419/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                      >  2:4153491/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                      <  1:3678818/93‑1 (MQ=255)

                     cTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCca                                                                     >  1:4850743/1‑93 (MQ=255)

                      ttCCTGATAATAACGCAGGCCCAGCGCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcag                                                                    <  1:760042/93‑1 (MQ=255)

                        ccTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagca                                                                  >  1:1428111/1‑93 (MQ=255)

                         cTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagcag                                                                 <  1:1653491/93‑1 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                                >  2:3890054/1‑93 (MQ=255)

                            ataataACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                              >  1:3207992/1‑93 (MQ=255)

                            ataataACGCAGGCCCAGCCCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                              <  2:2163255/93‑1 (MQ=255)

                              aataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCtt                                                            <  2:5027468/93‑1 (MQ=255)

                              aataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCtt                                                            >  2:4830226/1‑93 (MQ=255)

                               ataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTc                                                           <  2:1742894/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:1695334/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:579519/93‑1 (MQ=255)

                                taaCGCAGCCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:6542429/93‑1 (MQ=255)

                                 aaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCg                                                         <  1:228557/93‑1 (MQ=255)

                                   cGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCggg                                                       <  2:723241/93‑1 (MQ=255)

                                    gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGc                                                      >  2:1146008/1‑93 (MQ=255)

                                      cggCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg                                                    <  2:5435083/92‑1 (MQ=255)

                                      aGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGccg                                                    >  1:6409288/1‑91 (MQ=255)

                                      aGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg                                                    >  1:809563/1‑93 (MQ=255)

                                       ggCCCAGCTCGCTCATGCCAGGGCTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCCGCAGCTCTTCCGGGCGGt                                                   >  2:2410984/1‑93 (MQ=255)

                                        gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTg                                                  <  2:3627686/93‑1 (MQ=255)

                                         cccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGc                                                 >  2:3807738/1‑93 (MQ=255)

                                          ccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGcc                                                >  1:4636817/1‑93 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCa                                               <  1:2695495/93‑1 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCa                                               >  1:4466698/1‑93 (MQ=255)

                                            aGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                              <  1:6624472/93‑1 (MQ=255)

                                            aGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                              <  1:5793273/93‑1 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                             >  2:5881337/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                             >  2:5330111/1‑93 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                           <  2:3945158/93‑1 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                           <  2:5177835/93‑1 (MQ=255)

                                                cgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCgg                                          <  2:3248371/93‑1 (MQ=255)

                                                 gctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCggg                                         <  1:5908292/93‑1 (MQ=255)

                                                  ctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGt                                        <  1:6599209/93‑1 (MQ=255)

                                                  ctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGt                                        <  1:5392893/93‑1 (MQ=255)

                                                   tcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTa                                       <  2:3451209/93‑1 (MQ=255)

                                                     aTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAg                                     <  2:1501601/93‑1 (MQ=255)

                                                     aTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAg                                     <  1:2549787/93‑1 (MQ=255)

                                                       gCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc                                   <  1:6178550/93‑1 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                  >  2:2586989/1‑93 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                  >  2:1409140/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                >  1:4541941/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                <  1:2608397/93‑1 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                <  2:1383768/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                               <  2:590446/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                               >  2:598179/1‑93 (MQ=255)

                                                            ggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCg                              >  2:5588454/1‑93 (MQ=255)

                                                             gTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGa                             <  2:4025518/93‑1 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCCCTCGGGTAAGGCTTTCGAc                            >  1:54449/1‑93 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc                            >  2:405844/1‑93 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc                            >  1:1354836/1‑93 (MQ=255)

                                                                 tCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACata                         <  2:5003388/93‑1 (MQ=255)

                                                                 tCGCCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACata                         <  1:1010645/93‑1 (MQ=255)

                                                                  cGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACatat                        <  1:976052/93‑1 (MQ=255)

                                                                  cGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACatat                        <  2:2308063/93‑1 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                       >  2:1243562/1‑93 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                       <  2:21921/93‑1 (MQ=255)

                                                                    gCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTg                      >  2:4304745/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                     >  2:3059600/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCCGGGCAGGCTTTCGACATATTgg                     >  1:5215911/1‑92 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                    >  2:5707482/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                    >  2:5280583/1‑93 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa                >  2:2079283/1‑93 (MQ=255)

                                                                            cTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATc              <  1:2704497/93‑1 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca             <  2:1801122/93‑1 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTGCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca             <  1:1104273/93‑1 (MQ=255)

                                                                              gATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcac            >  2:5849988/1‑93 (MQ=255)

                                                                              gATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcac            >  2:4671731/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg           >  2:3697138/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGATCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg           >  1:2981640/1‑93 (MQ=255)

                                                                                ttCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGTCATATTGCGGGTATcacgc          <  1:6458176/93‑1 (MQ=255)

                                                                                ttCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgc          >  1:472885/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca         >  1:687656/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca         <  2:2457989/93‑1 (MQ=255)

                                                                                   tGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcacg       <  2:5356473/93‑1 (MQ=255)

                                                                                   tGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcacg       <  1:6055698/93‑1 (MQ=255)

                                                                                    gTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGt      <  2:2017206/93‑1 (MQ=255)

                                                                                       tttACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgc   <  2:4902023/93‑1 (MQ=255)

                                                                                        ttGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgcg  <  1:2091172/93‑1 (MQ=255)

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG  >  NC_000913/4582960‑4583140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`