BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,429	T→C	R178R (CGA→CGG)	ybhO ←	putative synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,429	0	T	C	88.5%	563.6 / 57.0	210	R178R (CGA→CGG)	ybhO	putative synthetase
Reads supporting (aligned to +/- strand): ref base T (13/11); new base C (87/98); total (101/109)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

Only 100 of 222 total aligned reads displayed.

ATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCG  >  NC_000913/822340‑822520

 tatCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACgccgcc                                                                                         >  2:3648942/1‑93 (MQ=255)

 tatCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACgccgcc                                                                                         >  2:4141281/1‑93 (MQ=255)

  atcatcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGcca                                                                                        >  1:229613/1‑93 (MQ=255)

  atcatcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGcca                                                                                        >  1:4783102/1‑93 (MQ=255)

   tcatcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGccac                                                                                       >  2:6121589/1‑93 (MQ=255)

   tcatcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGccac                                                                                       >  1:4647282/1‑93 (MQ=255)

   tcatcGCGATGTTCTTCGTTATCGCACCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACgccgcccc                                                                                       <  2:6422469/93‑3 (MQ=255)

    catcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGccacc                                                                                      >  1:5608666/1‑93 (MQ=255)

    catcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGccacc                                                                                      <  1:2062709/93‑1 (MQ=255)

     atcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGccaccc                                                                                     >  2:288111/1‑92 (MQ=255)

      tcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAg                                                                                    >  1:6039499/1‑93 (MQ=255)

      tcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAg                                                                                    <  2:773923/93‑1 (MQ=255)

      tcGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATCACGTGGCCACCAg                                                                                    <  1:6533553/93‑1 (MQ=255)

       cgcgATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGc                                                                                   <  1:5125463/93‑1 (MQ=255)

       cgcgATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGc                                                                                   <  2:2913400/93‑1 (MQ=255)

        gcgATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGcg                                                                                  >  1:1299649/1‑93 (MQ=255)

        gcgATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGcg                                                                                  >  2:2824126/1‑93 (MQ=255)

         cgATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGcgt                                                                                 <  2:6004929/93‑1 (MQ=255)

          gATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGcgtc                                                                                >  2:1640001/1‑93 (MQ=255)

            tGTTCTTCGTTATCGCGCCAGACCTTCAATACCTGCGCTTCTCCCGGCTGGCGGTGCTCTTCCGCCTTGTGATGACGCCGCCACCAGcgtcgt                                                                              >  1:6640677/1‑93 (MQ=255)

            tGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGcgtcgt                                                                              <  1:5235330/93‑1 (MQ=255)

               tcttcGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGcc                                                                           >  2:6510659/1‑93 (MQ=255)

               tcttcGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGcc                                                                           >  2:6532527/1‑93 (MQ=255)

                cttcGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCcg                                                                          <  2:2248932/93‑1 (MQ=255)

                  tcGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCcgcg                                                                        >  1:6280166/1‑93 (MQ=255)

                   tgTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCgcgc                                                                       <  1:4758506/92‑1 (MQ=255)

                   cGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCgcgc                                                                       <  1:3457226/93‑1 (MQ=255)

                      tATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGctct                                                                    <  1:4842502/93‑1 (MQ=255)

                          gcgcCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTcc                                                                <  2:4844186/93‑1 (MQ=255)

                          gcgcCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCACCGTCGTGCCGCGCTCTGTcc                                                                <  2:1954726/93‑1 (MQ=255)

                           cgcCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCca                                                               <  1:2642151/93‑1 (MQ=255)

                           cgcCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCca                                                               <  1:4245304/93‑1 (MQ=255)

                            gcCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTcccg                                                              >  1:6353455/1‑91 (MQ=255)

                              cAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggc                                                            >  2:1210204/1‑93 (MQ=255)

                               aGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggca                                                           <  2:1829024/93‑1 (MQ=255)

                                gACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggcag                                                          >  1:4322290/1‑93 (MQ=255)

                                 aCCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggcagg                                                         >  1:2351603/1‑93 (MQ=255)

                                 aCCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggcagg                                                         >  2:2347940/1‑93 (MQ=255)

                                  ccAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGt                                                        >  2:5924323/1‑93 (MQ=255)

                                   cAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGtt                                                       >  2:3017062/1‑93 (MQ=255)

                                     gCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGtttt                                                     >  1:2112515/1‑93 (MQ=255)

                                     gCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGgttt                                                     >  2:4044057/1‑93 (MQ=255)

                                     acAATGCCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGtttt                                                     <  1:1755810/92‑1 (MQ=255)

                                      cAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTc                                                    <  2:6285841/93‑1 (MQ=255)

                                      cAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTc                                                    <  2:5269484/93‑1 (MQ=255)

                                       aaTACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTcc                                                   >  2:55932/1‑93 (MQ=255)

                                        aTACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCa                                                  >  1:273437/1‑93 (MQ=255)

                                         tgccTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAg                                                 <  2:3498361/91‑1 (MQ=255)

                                           ccTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCt                                               <  2:1463176/93‑1 (MQ=255)

                                           ccTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCt                                               >  1:2841378/1‑93 (MQ=255)

                                           ccTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCt                                               <  2:6589681/93‑1 (MQ=255)

                                             tGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCa                                             >  1:3041885/1‑93 (MQ=255)

                                             tGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCa                                             >  2:436447/1‑93 (MQ=255)

                                                gcTTCTCCGGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAAc                                          <  2:3571484/93‑1 (MQ=255)

                                                gcTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAAc                                          >  2:3919043/1‑93 (MQ=255)

                                                 cTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAACTCAAACt                                         >  1:303815/1‑93 (MQ=255)

                                                      cccGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGgaga                                    <  2:1507302/93‑1 (MQ=255)

                                                      cccGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGgaga                                    >  1:5517279/1‑93 (MQ=255)

                                                      cccGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGgaga                                    <  2:5036087/93‑1 (MQ=255)

                                                         ggCTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAata                                 <  1:3519144/93‑1 (MQ=255)

                                                           cTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATc                               <  1:4072238/93‑1 (MQ=255)

                                                           cTGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATc                               <  1:4040087/93‑1 (MQ=255)

                                                           cTGGCGGTGCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATc                               <  1:1390913/93‑1 (MQ=255)

                                                            tGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCt                              >  1:5744233/1‑93 (MQ=255)

                                                            tGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCt                              >  2:3256140/1‑93 (MQ=255)

                                                            tGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCt                              <  2:685258/93‑1 (MQ=255)

                                                            tGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCt                              >  1:1560858/1‑93 (MQ=255)

                                                            tGGCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCt                              >  2:3805724/1‑93 (MQ=255)

                                                              gCGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTc                            <  1:5181935/93‑1 (MQ=255)

                                                               cGGTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCg                           <  2:3701478/93‑1 (MQ=255)

                                                                ggTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGa                          >  1:1462002/1‑93 (MQ=255)

                                                                 gTTCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGAc                         <  2:2170763/93‑1 (MQ=255)

                                                                  ttCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACa                        <  1:5639211/93‑1 (MQ=255)

                                                                  ttCTCTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGACACTATCTTCGata                        >  1:5871401/1‑91 (MQ=255)

                                                                   tctcTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACaa                       <  2:429103/93‑1 (MQ=255)

                                                                   tctcTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACaa                       <  2:5423352/93‑1 (MQ=255)

                                                                   tctcTTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACaa                       <  2:1071369/93‑1 (MQ=255)

                                                                    ctctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAAt                      <  2:4569699/93‑1 (MQ=255)

                                                                     tctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATc                     <  2:4783094/93‑1 (MQ=255)

                                                                     tctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATc                     <  2:4065202/93‑1 (MQ=255)

                                                                     tctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATc                     >  1:1054120/1‑93 (MQ=255)

                                                                      ctcccGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCg                    <  1:5992302/90‑1 (MQ=255)

                                                                      ctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCg                    <  2:102863/93‑1 (MQ=255)

                                                                      ctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCg                    <  2:571439/93‑1 (MQ=255)

                                                                      ctTCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCg                    >  2:3452419/1‑93 (MQ=255)

                                                                        tCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGc                  <  1:3181164/93‑1 (MQ=255)

                                                                        tCCGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGc                  <  1:3405625/93‑1 (MQ=255)

                                                                         ccGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGcc                 <  2:827687/93‑1 (MQ=255)

                                                                         ccGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGcc                 <  1:2229733/93‑1 (MQ=255)

                                                                         ccGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGCTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGcc                 >  2:6132808/1‑93 (MQ=255)

                                                                          cGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGccc                >  2:6346750/1‑93 (MQ=255)

                                                                          cGCTTTGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTGCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGccc                <  1:6556640/93‑1 (MQ=255)

                                                                             tttGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTc             >  1:5033468/1‑93 (MQ=255)

                                                                              ttGTGATGACGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCa            >  2:5759551/1‑93 (MQ=255)

                                                                                  gatgaCGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGc        <  1:3255953/93‑1 (MQ=255)

                                                                                  gatgaCGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGc        >  1:1934221/1‑93 (MQ=255)

                                                                                     gaCGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTa     <  1:3139063/93‑1 (MQ=255)

                                                                                      aCGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTAc    <  1:6224072/93‑1 (MQ=255)

                                                                                      aCGCCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTAc    >  1:5464139/1‑93 (MQ=255)

                                                                                        gTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACcg  >  2:2494059/1‑93 (MQ=255)

ATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCG  >  NC_000913/822340‑822520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`