BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,573,190	Δ1 bp	coding (1487/1542 nt)	yjiU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,189	0	G	.	99.5%	1912.4 / ‑1.4	422	coding (1486/1542 nt)	yjiU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (227/193); total (228/194)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 451 total aligned reads displayed.

TTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCC  >  NC_000913/4573097‑4573277

 tatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAga                                                                                         >  2:5930193/1‑92 (MQ=255)

   tCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAgagc                                                                                       >  2:5118516/1‑90 (MQ=255)

   tCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAgagc                                                                                       >  1:2266590/1‑90 (MQ=255)

    cAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAgagca                                                                                      >  2:3018438/1‑89 (MQ=255)

     aaTACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCat                                                                                    <  1:6931938/93‑1 (MQ=255)

     aaTACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCat                                                                                    >  1:4348789/1‑93 (MQ=255)

     aaTACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCat                                                                                    >  1:7222125/1‑93 (MQ=255)

      aTACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCata                                                                                   <  1:4439470/93‑1 (MQ=255)

       tACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCatat                                                                                  >  1:4239447/1‑93 (MQ=255)

         cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGa                                                                                >  1:7736054/1‑93 (MQ=255)

         cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGa                                                                                <  1:5078337/93‑1 (MQ=255)

         cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGa                                                                                >  1:7835994/1‑93 (MQ=255)

          aTGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGat                                                                               >  2:2555855/1‑92 (MQ=255)

          aTGGCTGAAAGAAGCATGGGCCCAACCTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGAc                                                                               <  1:8495072/93‑1 (MQ=255)

             gCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACttt                                                                            <  2:4380890/93‑1 (MQ=255)

              cTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACtttt                                                                           <  1:2164276/93‑1 (MQ=255)

              cTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACtttt                                                                           <  2:8752846/93‑1 (MQ=255)

               tGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACttttt                                                                          >  1:6385546/1‑93 (MQ=255)

                gAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTc                                                                         >  1:8349922/1‑93 (MQ=255)

                  aagaagCATGGGCCCATCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCtt                                                                       <  2:8943435/93‑1 (MQ=255)

                  aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCtt                                                                       <  1:3486475/93‑1 (MQ=255)

                  aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCtt                                                                       >  1:1243140/1‑93 (MQ=255)

                   agaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCttt                                                                      >  1:4532030/1‑93 (MQ=255)

                   agaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCttt                                                                      >  1:406172/1‑93 (MQ=255)

                    gaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTa                                                                     >  2:9286913/1‑93 (MQ=255)

                    gaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTa                                                                     <  2:5374963/93‑1 (MQ=255)

                      agCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAAt                                                                   <  1:758761/93‑1 (MQ=255)

                      agCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAAt                                                                   >  2:4105102/1‑93 (MQ=255)

                       gCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATc                                                                  >  2:2212932/1‑93 (MQ=255)

                         aTGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCAc                                                                >  1:8599979/1‑93 (MQ=255)

                          tGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                               >  2:8338497/1‑93 (MQ=255)

                          tGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                               >  1:7092122/1‑93 (MQ=255)

                            ggCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGa                                                             <  2:8088937/93‑1 (MQ=255)

                            ggCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGa                                                             <  1:5953466/93‑1 (MQ=255)

                            ggCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGa                                                             <  2:6411208/93‑1 (MQ=255)

                              cccAACTTACCGGACCGCTTGCAGTACTGAAACCCGATCGCAGCCGTTTTTATTTACCGAAAGG‑GCATATGACTTTTTCTTTAATCACGGATg                                                           >  1:8039807/1‑93 (MQ=255)

                               ccAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGc                                                          <  2:8188083/93‑1 (MQ=255)

                                 aaCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGccg                                                        >  1:1364051/1‑92 (MQ=255)

                                 aaCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCt                                                        <  1:576284/93‑1 (MQ=255)

                                 aaCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCt                                                        >  1:266460/1‑93 (MQ=255)

                                  aCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTg                                                       >  2:8635036/1‑93 (MQ=255)

                                    ttACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGa                                                     >  2:3199901/1‑93 (MQ=255)

                                     tACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAt                                                    >  1:1629456/1‑93 (MQ=255)

                                      aCCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAtt                                                   <  2:5722179/93‑1 (MQ=255)

                                       ccGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAttt                                                  >  2:3632743/1‑93 (MQ=255)

                                         ggACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGc                                                >  1:1805146/1‑93 (MQ=255)

                                          gACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGcc                                               <  1:2461587/93‑1 (MQ=255)

                                          gACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGcc                                               <  2:745433/93‑1 (MQ=255)

                                           aCCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGccc                                              <  2:62946/93‑1 (MQ=255)

                                            ccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCg                                             <  2:1321977/93‑1 (MQ=255)

                                            ccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCg                                             >  2:4676515/1‑93 (MQ=255)

                                              gCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGt                                           >  2:1104081/1‑93 (MQ=255)

                                              gCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGt                                           >  1:4646146/1‑93 (MQ=255)

                                              gCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGt                                           <  2:1933553/93‑1 (MQ=255)

                                              gCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGt                                           >  2:6795635/1‑93 (MQ=255)

                                               cTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTa                                          >  2:5465319/1‑93 (MQ=255)

                                               cTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTa                                          <  2:1872020/93‑1 (MQ=255)

                                                ttGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                         <  1:8884347/93‑1 (MQ=255)

                                                ttGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                         >  2:9472633/1‑93 (MQ=255)

                                                ttGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                         >  1:106106/1‑93 (MQ=255)

                                                 tGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                        >  2:2650532/1‑93 (MQ=255)

                                                  gCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAcc                                       <  2:2807687/93‑1 (MQ=255)

                                                  gCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAcc                                       >  2:1756050/1‑93 (MQ=255)

                                                     gTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAAt                                    <  1:2858197/93‑1 (MQ=255)

                                                      tACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATa                                   >  2:243084/1‑93 (MQ=255)

                                                      tACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATa                                   >  1:3163213/1‑93 (MQ=255)

                                                        cTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaa                                 >  1:6814483/1‑93 (MQ=255)

                                                         tGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaaa                                >  2:7276438/1‑93 (MQ=255)

                                                         tGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaaa                                >  2:9239982/1‑93 (MQ=255)

                                                             aCCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCt                            >  2:5609709/1‑93 (MQ=255)

                                                              cccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTCATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTg                           >  2:5005685/1‑93 (MQ=255)

                                                              cccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTg                           <  1:9993004/93‑1 (MQ=255)

                                                              cccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTg                           <  1:9815363/93‑1 (MQ=255)

                                                              cccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTg                           <  1:3021706/93‑1 (MQ=255)

                                                               ccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTgg                          >  1:3530795/1‑93 (MQ=255)

                                                                cGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGa                         <  1:8698041/93‑1 (MQ=255)

                                                                  aTGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                       <  1:3757103/93‑1 (MQ=255)

                                                                  aTGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                       >  1:9626007/1‑93 (MQ=255)

                                                                  aTGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                       <  1:2005342/93‑1 (MQ=255)

                                                                  aTGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                       >  2:7042825/1‑93 (MQ=255)

                                                                   tGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATAc                      >  1:6635506/1‑93 (MQ=255)

                                                                     gCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACgg                    >  1:929586/1‑93 (MQ=255)

                                                                      cAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGc                   <  2:3473183/93‑1 (MQ=255)

                                                                          cGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCtttt               >  2:6150478/1‑93 (MQ=255)

                                                                           gTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTa              >  2:847901/1‑93 (MQ=255)

                                                                           gTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTa              <  1:3286751/93‑1 (MQ=255)

                                                                            tttttATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaa             >  2:4783207/1‑93 (MQ=255)

                                                                            tttttATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaa             <  2:3898981/93‑1 (MQ=255)

                                                                             ttttATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaaa            <  2:9594962/93‑1 (MQ=255)

                                                                              tttatttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATAAGGCTTTTAAAg           >  1:6135582/1‑93 (MQ=255)

                                                                                tatttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGc         <  1:5448166/93‑1 (MQ=255)

                                                                                  tttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt       >  2:7728791/1‑93 (MQ=255)

                                                                                  tttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt       <  2:688470/93‑1 (MQ=255)

                                                                                  tttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt       <  2:1649357/93‑1 (MQ=255)

                                                                                  tttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTGGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt       <  1:7546653/93‑1 (MQ=255)

                                                                                   ttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTa      <  1:8241947/93‑1 (MQ=255)

                                                                                    taCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTaa     >  1:5879684/1‑93 (MQ=255)

                                                                                    taCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTaa     <  2:7921999/93‑1 (MQ=255)

                                                                                    taCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTaa     <  2:9771006/93‑1 (MQ=255)

                                                                                     aCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAc    <  1:4902232/93‑1 (MQ=255)

TTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCC  >  NC_000913/4573097‑4573277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`