BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,003,385	Δ1 bp	coding (186/681 nt)	ycbF →	putative chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,003,384	0	A	.	88.8%	707.3 / 75.4	206	coding (185/681 nt)	ycbF	putative chaperone
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (119/64); minor base G (12/11); total (131/75)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-02

Only 100 of 235 total aligned reads displayed.

AGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAA  >  NC_000913/1003292‑1003473

 gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGGag                                                                                          >  2:4775400/1‑92 (MQ=255)

   aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGGGAGc                                                                                        >  1:3481190/1‑93 (MQ=255)

      gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCCACCGCCCCAC‑GCGTTGGGCCGGGGGGG‑AGCGTca                                                                                    >  1:1265852/1‑92 (MQ=255)

      gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCg                                                                                    >  2:6532288/1‑93 (MQ=255)

       atatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGa                                                                                   <  1:1301201/93‑1 (MQ=255)

       atatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGa                                                                                   >  2:9195902/1‑93 (MQ=255)

        tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGAc                                                                                  >  1:5865793/1‑93 (MQ=255)

         atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACa                                                                                 >  2:6591525/1‑93 (MQ=255)

         atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACa                                                                                 >  1:6064901/1‑93 (MQ=255)

         atCTATTTAAGGGACTAGTACGAGTCAGGAAGCGTGGCTGATTAGCAGTAAATACACCGTCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACa                                                                                 <  2:5013940/93‑1 (MQ=255)

          tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑GGCGTCGACAg                                                                                >  2:3749487/1‑93 (MQ=255)

           cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGt                                                                               >  2:9648603/1‑93 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGGGAGCGTCGACAGt                                                                               >  1:9840881/1‑93 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTg                                                                              >  1:579434/1‑93 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTg                                                                              <  1:5237066/93‑1 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTg                                                                              >  1:2650358/1‑93 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTg                                                                              >  2:2187289/1‑93 (MQ=255)

            tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTg                                                                              >  1:4201741/1‑93 (MQ=255)

             tttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGc                                                                             <  1:4828423/92‑1 (MQ=255)

              ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGcc                                                                            >  1:4340453/1‑93 (MQ=255)

                ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAg                                                                          >  2:9626112/1‑93 (MQ=255)

                ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAg                                                                          >  1:3662093/1‑93 (MQ=255)

                  tcTGACCAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCa                                                                        <  1:1065218/92‑1 (MQ=255)

                  aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCa                                                                        >  2:3358436/1‑93 (MQ=255)

                     gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCa                                                                     <  1:4974177/93‑1 (MQ=255)

                     gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCa                                                                     <  2:3423119/93‑1 (MQ=255)

                     gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCa                                                                     >  1:2238474/1‑93 (MQ=255)

                        tAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTa                                                                  >  1:5964082/1‑93 (MQ=255)

                         aaTACCAGTCAGGAATCCTGTCTTATAAACAGTAAAATCACCCGCCCAAC‑GGGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTAc                                                                 <  2:8452388/93‑1 (MQ=255)

                          aTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACt                                                                >  2:9663329/1‑93 (MQ=255)

                           tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTg                                                               >  1:9575780/1‑93 (MQ=255)

                           aaCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTg                                                               <  2:6417741/92‑1 (MQ=255)

                             ccAGTGAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGc                                                             <  2:7823055/93‑1 (MQ=255)

                               aGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGccc                                                           >  1:3083211/1‑92 (MQ=255)

                                gTCAGGAATCCTGGCTTATAAACAGTAAAATCAACCGCCCAAC‑GGGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGACGc                                                          <  1:6254923/93‑1 (MQ=255)

                                 tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                         >  1:7348410/1‑93 (MQ=255)

                                 tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                         >  1:1919354/1‑93 (MQ=255)

                                   agaaaTCCGGGTTTATAAAAATAAAAACCACCCGCCCAAC‑GCTTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                       <  2:9476113/90‑1 (MQ=255)

                                   aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                       <  1:8470737/93‑1 (MQ=255)

                                    ggAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg                                                      >  1:6488188/1‑93 (MQ=255)

                                     gAATCCTGGCTTATTGACACTAAAATCAACCGCCCAAC‑CCGATGGGCCGGGGGGG‑CGCCTCCGCCGAGCCAGCACCCTTACCGGGCGCCCCGc                                                     >  2:2111335/1‑93 (MQ=255)

                                       aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGG‑‑AGCCTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                                  >  2:4488878/1‑93 (MQ=255)

                                        tCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                                  <  2:6879093/93‑1 (MQ=255)

                                        tCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                                  >  2:2784858/1‑93 (MQ=255)

                                         ccTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACt                                                 >  1:475187/1‑93 (MQ=255)

                                         ccTGGCTTATTAACAGAAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACt                                                 <  1:8372651/93‑1 (MQ=255)

                                          cTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt                                                >  1:3291910/1‑93 (MQ=255)

                                            ggCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                              >  2:9099418/1‑93 (MQ=255)

                                            ggCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                              >  2:4777289/1‑93 (MQ=255)

                                            ggCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                              >  1:4929602/1‑93 (MQ=255)

                                               ttattaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtct                                           >  1:9199603/1‑93 (MQ=255)

                                                tattaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtctc                                          >  2:9610668/1‑93 (MQ=255)

                                                 attaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTcc                                         >  1:8796054/1‑93 (MQ=255)

                                                  ttaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                        >  2:5245894/1‑93 (MQ=255)

                                                  ttaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                        >  2:3980093/1‑93 (MQ=255)

                                                  ttaACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                        >  2:6319752/1‑93 (MQ=255)

                                                    aaCAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGa                                      >  1:9915344/1‑93 (MQ=255)

                                                    aaCAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGGGCCAGCACCATACCTGGCCGCTCCCCCCCTTATtctcttta                                      >  2:6092860/1‑89 (MQ=255)

                                                       aGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGc                                   >  1:2506038/1‑93 (MQ=255)

                                                         aaaaaTCAACCGCCCAAC‑GGGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                                 <  2:3080475/92‑1 (MQ=255)

                                                          aaaaTCACCCGCCCAAG‑GCGTTGGGCCGGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                                 <  1:5336383/93‑1 (MQ=255)

                                                          aaaaTCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCg                                <  1:5004741/93‑1 (MQ=255)

                                                            aaTCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGt                              >  2:8065891/1‑93 (MQ=255)

                                                             accAACCCCCCAGC‑G‑GTTGGGGCCGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTa                             <  1:2337087/91‑1 (MQ=255)

                                                             aTCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTa                             >  2:6474207/1‑93 (MQ=255)

                                                             aTCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTa                             >  2:6869085/1‑93 (MQ=255)

                                                             aTCAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTa                             >  2:8307329/1‑93 (MQ=255)

                                                             aTCAACCCCCCAACGGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGt                              <  2:4337854/93‑1 (MQ=255)

                                                              acAACCGCCCACC‑GCGTTGGGCCGGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTa                             <  1:1467968/92‑1 (MQ=255)

                                                               cAACCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACg                           >  1:8123036/1‑93 (MQ=255)

                                                                aaCCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                          >  1:6546445/1‑93 (MQ=255)

                                                                aaCCGCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGCACGa                          >  1:3340188/1‑93 (MQ=255)

                                                                 aCCGCCCACC‑GCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGAc                         <  2:1447215/93‑1 (MQ=255)

                                                                  ccccccACC‑GCGTTGGCCCGGGGGGG‑‑GCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                       <  2:2866999/90‑1 (MQ=255)

                                                                  ccGCCCACC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACa                        <  1:2658072/93‑1 (MQ=255)

                                                                   cGCCTAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                       <  1:1668510/93‑1 (MQ=255)

                                                                    gCCCAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAgg                      >  2:3579502/1‑93 (MQ=255)

                                                                     cccAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGc                     >  2:6101856/1‑93 (MQ=255)

                                                                     cacAAC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGATCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGc                     <  2:5277240/91‑1 (MQ=255)

                                                                      ccACC‑GGGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa                    <  2:8422991/93‑1 (MQ=255)

                                                                         aC‑GCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                 >  1:9004479/1‑93 (MQ=255)

                                                                            gcgTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTg               >  2:4598438/1‑93 (MQ=255)

                                                                            gcgTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTg               <  1:3588448/93‑1 (MQ=255)

                                                                              gTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg             >  2:1751079/1‑93 (MQ=255)

                                                                              gTTGGGCCCGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgc              <  2:2475726/93‑1 (MQ=255)

                                                                               ttGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcgc            <  2:10014209/93‑1 (MQ=255)

                                                                                tGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt           <  2:4988894/93‑1 (MQ=255)

                                                                                ggggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt           <  2:3021462/92‑1 (MQ=255)

                                                                                 gggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCtt          >  1:7023998/1‑93 (MQ=255)

                                                                                  ggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTg         <  1:4887026/93‑1 (MQ=255)

                                                                                   gCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGc        <  2:1329072/93‑1 (MQ=255)

                                                                                   gCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGc        >  2:3163394/1‑93 (MQ=255)

                                                                                     gcggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCt       <  1:2834143/91‑1 (MQ=255)

                                                                                       ggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTga     <  2:492155/93‑1 (MQ=255)

                                                                                       ggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTga     >  2:7280146/1‑93 (MQ=255)

                                                                                       ggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTga     <  2:2039221/93‑1 (MQ=255)

                                                                                       cggggggggCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTga     <  1:9710324/92‑1 (MQ=255)

                                                                                        ggggggAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTgag    >  2:710450/1‑93 (MQ=255)

                                                                                          ggggAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGaa  >  1:945328/1‑93 (MQ=255)

                                                                                          ggggAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGaa  <  2:9773666/93‑1 (MQ=255)

AGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAC‑GCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAA  >  NC_000913/1003292‑1003473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`