BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,067,441	(A)_6→5	coding (138/174 nt)	b4516 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,067,436	0	A	.	98.8%	1086.8 / 5.2	244	coding (133/174 nt)	b4516	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (116/124); total (119/125)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA  >  NC_000913/1067344‑1067524

   gcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaa                                                                                       >  1:9757320/1‑93 (MQ=255)

   gcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaa                                                                                       <  2:6607041/93‑1 (MQ=255)

    cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaa                                                                                      <  2:2204994/93‑1 (MQ=255)

    cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaa                                                                                      <  2:3946201/93‑1 (MQ=255)

      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    >  1:9186932/1‑91 (MQ=255)

      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    <  1:2185149/93‑3 (MQ=255)

      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    >  2:6512756/1‑91 (MQ=255)

       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   >  1:8905001/1‑90 (MQ=255)

       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   <  1:9952714/93‑4 (MQ=255)

       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   <  1:4357930/93‑4 (MQ=255)

        agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggtg                                                                                  <  1:109306/93‑5 (MQ=255)

        agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggtg                                                                                  >  1:9433577/1‑89 (MQ=255)

           gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTa                                                                              <  2:7373684/93‑1 (MQ=255)

             aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAag                                                                            <  2:8362692/93‑1 (MQ=255)

              tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAaga                                                                           >  1:2874481/1‑93 (MQ=255)

              tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAaga                                                                           >  1:2321346/1‑93 (MQ=255)

                aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGc                                                                         >  1:5837224/1‑93 (MQ=255)

                 gAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCa                                                                        <  1:444133/93‑1 (MQ=255)

                  aaGCAGGTAAAAAAGGTGGACAGCACAGCGGGGTTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAg                                                                       <  2:5429439/93‑1 (MQ=255)

                     cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCa                                                                    <  2:8778695/93‑1 (MQ=255)

                       ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACg                                                                  <  2:4542128/93‑1 (MQ=255)

                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACgg                                                                 >  2:240345/1‑93 (MQ=255)

                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACgg                                                                 <  2:5516128/93‑1 (MQ=255)

                         tAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGc                                                                <  2:1382704/93‑1 (MQ=255)

                          aaaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCa                                                               <  2:9876951/93‑1 (MQ=255)

                           aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              <  1:9464903/93‑1 (MQ=255)

                           aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              >  1:1337610/1‑93 (MQ=255)

                           aaaaaGGTGGACAGAACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              >  1:6847181/1‑93 (MQ=255)

                             aaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaaaa                                                            <  1:7045926/93‑1 (MQ=255)

                             aaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaaaa                                                            <  1:7369277/93‑1 (MQ=255)

                              aaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAg                                                           >  2:47182/1‑93 (MQ=255)

                                 gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGa                                                        <  1:8409068/93‑1 (MQ=255)

                                   ggACAGCGGAGCGGGGGTAATTTCAAGAATGACCAGCAGCGCGCATCTGAAGCA‑GCAAAAAAGGTGGTAAGATCAGTCACGGCAAAAGCGACa                                                      <  1:5567983/93‑1 (MQ=255)

                                   ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACa                                                      >  2:8194605/1‑93 (MQ=255)

                                   ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACa                                                      >  2:5387756/1‑93 (MQ=255)

                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:1598100/93‑1 (MQ=255)

                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:9173013/93‑1 (MQ=255)

                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:7823681/93‑1 (MQ=255)

                                     acagcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAAc                                                    >  2:3011216/1‑93 (MQ=255)

                                      cagcacagcGGGGGTAATTACAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACt                                                   >  2:5753191/1‑93 (MQ=255)

                                       agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTa                                                  >  2:5262651/1‑93 (MQ=255)

                                       agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTa                                                  <  2:3218189/93‑1 (MQ=255)

                                        gcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAg                                                 <  2:1527310/93‑1 (MQ=255)

                                         cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGc                                                <  1:3191910/93‑1 (MQ=255)

                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAgtc                                               >  1:6953250/1‑91 (MQ=255)

                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               <  2:369973/93‑1 (MQ=255)

                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               >  1:7345375/1‑93 (MQ=255)

                                           cagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               >  2:3602524/1‑93 (MQ=255)

                                              ggggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                           <  2:5065924/92‑1 (MQ=255)

                                              cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                           <  2:4450477/93‑1 (MQ=255)

                                                ggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                         <  2:787764/93‑1 (MQ=255)

                                                 gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTaa                                        <  1:8658014/93‑1 (MQ=255)

                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  2:8824828/93‑1 (MQ=255)

                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  2:768013/93‑1 (MQ=255)

                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  1:4009358/93‑1 (MQ=255)

                                                     aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCaa                                    <  1:8031204/93‑1 (MQ=255)

                                                     aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCaa                                    <  2:6779940/93‑1 (MQ=255)

                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   <  2:8546139/93‑1 (MQ=255)

                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   <  1:606473/93‑1 (MQ=255)

                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   >  1:3770171/1‑93 (MQ=255)

                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  2:2571292/1‑93 (MQ=255)

                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  1:3545984/1‑93 (MQ=255)

                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  2:5881251/1‑93 (MQ=255)

                                                         tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAc                                <  1:4085859/93‑1 (MQ=255)

                                                         tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAc                                <  1:188533/93‑1 (MQ=255)

                                                            aGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGaa                             <  1:5472760/93‑1 (MQ=255)

                                                             gAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAt                            <  1:7868332/93‑1 (MQ=255)

                                                                tGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCa                         >  1:8107298/1‑93 (MQ=255)

                                                                  aCCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtt                       >  2:3866089/1‑93 (MQ=255)

                                                                   cccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                      >  2:4129659/1‑93 (MQ=255)

                                                                   cccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                      <  1:1701998/93‑1 (MQ=255)

                                                                    ccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtttt                     >  2:6066682/1‑93 (MQ=255)

                                                                     cGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                    >  2:5700832/1‑93 (MQ=255)

                                                                      gCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTg                   <  2:7115128/93‑1 (MQ=255)

                                                                      gCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTg                   >  2:3452570/1‑93 (MQ=255)

                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  2:9358167/93‑1 (MQ=255)

                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 >  1:3052713/1‑93 (MQ=255)

                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  2:8607570/93‑1 (MQ=255)

                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  1:627319/93‑1 (MQ=255)

                                                                         gcgcgcATCTGAAGCAGGT‑AAAACGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                <  2:5604677/93‑1 (MQ=255)

                                                                         gcgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                <  2:2941009/93‑1 (MQ=255)

                                                                         gcgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                >  1:5351304/1‑93 (MQ=255)

                                                                          cgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTg               >  2:3074691/1‑93 (MQ=255)

                                                                           gcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGt              <  1:1021183/93‑1 (MQ=255)

                                                                            cgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTa             >  2:2803697/1‑93 (MQ=255)

                                                                              cATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGc           <  2:1168219/93‑1 (MQ=255)

                                                                              cATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGc           <  1:5128575/93‑1 (MQ=255)

                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          >  1:7741771/1‑93 (MQ=255)

                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          >  1:4678812/1‑93 (MQ=255)

                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          <  1:6860168/93‑1 (MQ=255)

                                                                                tCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTc         >  1:566997/1‑93 (MQ=255)

                                                                                 cTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCg        <  2:4635011/93‑1 (MQ=255)

                                                                                  tGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGt       >  1:9287155/1‑93 (MQ=255)

                                                                                  tGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGt       <  2:1876239/93‑1 (MQ=255)

                                                                                   gAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGta      >  1:2504870/1‑92 (MQ=255)

                                                                                    aaGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCa     >  2:1999317/1‑93 (MQ=255)

                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:6973009/1‑93 (MQ=255)

                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:4980076/1‑93 (MQ=255)

                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:4056446/1‑93 (MQ=255)

                                                                                      gCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaaa   >  2:1272503/1‑93 (MQ=255)

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA  >  NC_000913/1067344‑1067524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`