Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,897,198 (C)5→6 coding (1041/1203 nt) yieL ← putative xylanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,1931.C97.3% 1284.5 / 22.0 407coding (1046/1203 nt)yieLputative xylanase
Reads supporting (aligned to +/- strand):  ref base . (5/6);  new base C (197/199);  total (202/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑CCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTC  >  NC_000913/3897101‑3897285
                                                                                              |                                                                                             
gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAt                                                                                                >  2:9104201/1‑93 (MQ=255)
 aCCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑c                                                                                              <  1:686357/93‑1 (MQ=255)
  cccGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑cc                                                                                             >  1:7912430/1‑93 (MQ=255)
   ccGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc                                                                                            >  2:7226517/1‑93 (MQ=255)
   ccGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc                                                                                            >  2:3955460/1‑93 (MQ=255)
    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑c                                                                                          >  2:4634922/1‑93 (MQ=255)
    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑c                                                                                          <  1:9322255/93‑1 (MQ=255)
    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑c                                                                                          >  1:6578527/1‑93 (MQ=255)
     ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cc                                                                                         >  2:9795489/1‑93 (MQ=255)
     ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cc                                                                                         <  2:3186800/93‑1 (MQ=255)
     ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cc                                                                                         >  2:1833545/1‑93 (MQ=255)
       atatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑ccca                                                                                       >  2:1086974/1‑91 (MQ=255)
         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cccaca                                                                                     >  1:6079153/1‑89 (MQ=255)
         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cccaca                                                                                     >  1:8613877/1‑89 (MQ=255)
         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑ccc‑cccaca                                                                                     <  2:2991016/93‑5 (MQ=255)
           tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAAc                                                                                    >  1:5108510/1‑93 (MQ=255)
             cTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACca                                                                                  <  1:6681873/93‑1 (MQ=255)
              tGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACcac                                                                                 >  1:3338521/1‑93 (MQ=255)
                gTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGACATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAg                                                                               <  1:4495672/93‑1 (MQ=255)
                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGt                                                                              >  1:8859383/1‑93 (MQ=255)
                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGt                                                                              <  1:9978789/93‑1 (MQ=255)
                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTa                                                                             <  1:769613/93‑1 (MQ=255)
                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTa                                                                             >  1:9899789/1‑93 (MQ=255)
                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTa                                                                             >  2:6943605/1‑93 (MQ=255)
                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTa                                                                             >  1:1039825/1‑93 (MQ=255)
                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTa                                                                             >  1:10018276/1‑93 (MQ=255)
                   aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTaa                                                                            >  2:2649360/1‑93 (MQ=255)
                    tCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTaaa                                                                           >  1:7300561/1‑93 (MQ=255)
                    tCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTaaa                                                                           >  1:2300221/1‑93 (MQ=255)
                     cAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTaaaa                                                                          >  2:675962/1‑93 (MQ=255)
                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAAt                                                                         <  2:8269226/93‑1 (MQ=255)
                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAAtt                                                                        >  2:2523827/1‑93 (MQ=255)
                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAAtt                                                                        >  2:7500148/1‑93 (MQ=255)
                        aGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTa                                                                       >  2:9351404/1‑93 (MQ=255)
                         gTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTAc                                                                      <  1:9118765/93‑1 (MQ=255)
                          ttAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACg                                                                     <  2:947116/93‑1 (MQ=255)
                           tAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGt                                                                    >  2:2141963/1‑93 (MQ=255)
                            aaTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTa                                                                   <  2:3524618/93‑1 (MQ=255)
                             aTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAg                                                                  >  2:124044/1‑93 (MQ=255)
                               ttttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGtt                                                                <  1:937113/93‑1 (MQ=255)
                                 ttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGc                                                              <  2:1628572/93‑1 (MQ=255)
                                 ttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGc                                                              <  2:4956705/93‑1 (MQ=255)
                                 ttttCTGCGCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGc                                                              <  2:6423921/93‑1 (MQ=255)
                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTgg                                                           <  1:9828311/93‑1 (MQ=255)
                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTgg                                                           <  2:8046105/93‑1 (MQ=255)
                                     cTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGt                                                          <  2:6152921/93‑1 (MQ=255)
                                     cTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGt                                                          <  2:2866620/93‑1 (MQ=255)
                                      tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGtt                                                         >  2:6584759/1‑93 (MQ=255)
                                       gCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTg                                                        >  2:3428439/1‑93 (MQ=255)
                                        cTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTATGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGa                                                       <  2:3289749/93‑1 (MQ=255)
                                        cTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGa                                                       >  2:7631579/1‑93 (MQ=255)
                                        cTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGa                                                       <  2:5116149/93‑1 (MQ=255)
                                         tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAt                                                      <  2:5459552/93‑1 (MQ=255)
                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGGTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                     >  1:8515631/1‑93 (MQ=255)
                                           aaGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAg                                                    >  1:8795748/1‑93 (MQ=255)
                                           aaGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAg                                                    >  1:4149614/1‑93 (MQ=255)
                                            agttCAGTTGTCAGCCCGGCGATATCCTTGCCTGTTAAGACATCTTGAAC‑CCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGc                                                   <  1:4351471/90‑1 (MQ=255)
                                            aGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCCCAGTAAAATTACGTAGTTGCTGGTTGATAGc                                                   <  2:3608334/93‑1 (MQ=255)
                                            aGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGc                                                   <  1:5998814/93‑1 (MQ=255)
                                              cTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTg                                                 >  2:4359746/1‑93 (MQ=255)
                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                                               >  2:8172846/1‑93 (MQ=255)
                                                 aGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCg                                              <  2:4138869/93‑1 (MQ=255)
                                                  gTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCgg                                             >  2:4761397/1‑93 (MQ=255)
                                                    tttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGAt                                           >  1:5520062/1‑93 (MQ=255)
                                                    tttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGAt                                           <  1:6199073/93‑1 (MQ=255)
                                                       cAGCCCGGTGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGt                                        <  2:1536193/93‑1 (MQ=255)
                                                        aGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGtt                                       >  1:5847793/1‑93 (MQ=255)
                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAACTTACGTAGTTGCTGGTTGATCGCTGCCGGATCGTTc                                      >  1:3566012/1‑93 (MQ=255)
                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTc                                      <  1:6429487/93‑1 (MQ=255)
                                                          cccGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCa                                     <  1:4233221/93‑1 (MQ=255)
                                                           ccGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAg                                    <  1:755601/93‑1 (MQ=255)
                                                            cGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGt                                   <  1:1516656/93‑1 (MQ=255)
                                                            cGGCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGt                                   >  1:3814387/1‑93 (MQ=255)
                                                              gCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTg                                                        <  1:1805729/70‑1 (MQ=255)
                                                              gCGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCg                                 <  2:8916730/93‑1 (MQ=255)
                                                               cGATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCgg                                <  1:3072581/93‑1 (MQ=255)
                                                                gATATCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCggg                               >  1:4018294/1‑93 (MQ=255)
                                                                 atatCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                              >  2:2306805/1‑93 (MQ=255)
                                                                 atatCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                              <  2:8637899/93‑1 (MQ=255)
                                                                 atatCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                              <  2:601835/93‑1 (MQ=255)
                                                                  tatCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                             >  1:4929816/1‑93 (MQ=255)
                                                                  tatCCTTGCCGATTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                             <  2:8571655/93‑1 (MQ=255)
                                                                    tCCTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                           >  1:275781/1‑93 (MQ=255)
                                                                      cTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGa                         >  1:5331697/1‑93 (MQ=255)
                                                                      cTTGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGa                         <  1:8251800/93‑1 (MQ=255)
                                                                        tGCCGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGaca                       >  2:5614079/1‑93 (MQ=255)
                                                                          ccGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                     <  2:3706105/93‑1 (MQ=255)
                                                                          ccGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                     >  1:7203772/1‑93 (MQ=255)
                                                                          ccGGTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                     <  2:7862254/93‑1 (MQ=255)
                                                                            ggTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCtt                   >  1:2875533/1‑93 (MQ=255)
                                                                             gTTACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc                  <  1:3441705/93‑1 (MQ=255)
                                                                              ttACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCa                 <  2:875588/93‑1 (MQ=255)
                                                                               tACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCAt                >  2:8826802/1‑93 (MQ=255)
                                                                               tACGACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCAt                <  2:6554805/93‑1 (MQ=255)
                                                                                  gACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCg             >  1:122338/1‑93 (MQ=255)
                                                                                  gACATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCg             >  1:2501808/1‑93 (MQ=255)
                                                                                    cATCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGt           >  1:9495281/1‑93 (MQ=255)
                                                                                     aTCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCTGTa          >  2:7069114/1‑93 (MQ=255)
                                                                                     aTCTT‑TAC‑CCCACCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTa          <  1:8077052/93‑1 (MQ=255)
                                                                                      tCTT‑TATCCCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc         >  1:5715146/1‑93 (MQ=255)
                                                                                              |                                                                                             
GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTT‑TAT‑CCC‑CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTC  >  NC_000913/3897101‑3897285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑